EMAGE mouse gene expression spatial database

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EMAGE:11496

Pck2 phosphoenolpyruvate carboxykinase 2 (mitochondrial) ( MGI:1860456)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006513. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006513_01	euxassay_006513_02	euxassay_006513_03	euxassay_006513_04

euxassay_006513_05	euxassay_006513_06	euxassay_006513_07	euxassay_006513_08	euxassay_006513_09

euxassay_006513_10	euxassay_006513_11	euxassay_006513_12	euxassay_006513_13	euxassay_006513_14

euxassay_006513_15	euxassay_006513_16	euxassay_006513_17	euxassay_006513_18	euxassay_006513_19

euxassay_006513_20	euxassay_006513_21	euxassay_006513_22	euxassay_006513_23	euxassay_006513_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11496_wholemount_strong.wlz

11496_wholemount_moderate.wlz

11496_wholemount_weak.wlz

11496_wholemount_possible.wlz

11496_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11496_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6605
Entity Detected:	Pck2, phosphoenolpyruvate carboxykinase 2 (mitochondrial) ( MGI:1860456)
Sequence:	sense strand is shown >T6605 TATGCTCCTTCCTTCCCCGCCTTCCTTGTCTCCCCGACTGCGCTCGTTCCTGTGGCCATCCCGCAGCGCC AGTCCAGGTGCCATGGCTGCTATGTACCTCCCCGGCCTGCGGCTTAGCCGGCACGGGCTAAGGCCCTGGT GCTGGTCACCGTGCCGTAGCATCCAAACCCTGCGTGTGCTCAGTGGAGATATGAGTCAGCTGCCGGCTGG AGTTCGAGACTTTGTGGCGCGCAGTGCGCATCTGTGCCAACCAGAGGGCATCCACATCTGTGATGGGACC GAGGCTGAGAACACTGCCATACTGGCCCTGCTGGAAGAACAGGGTCTTATCCGCAAACTCCCCAAGTATA AGAACTGCTGGCTGGCCCGCACAGACCCCAAGGATGTGGCACGGGTAGAAAGCAAGACGGTGATTGTAAC TCCTTCGCAGCGGGACACAGTGCCTCTCCTGGCTGGTGGGGCCAGGGGGCAGCTGGGCAACTGGATGTCC CCAGATGAGTTCCAGAGAGCTGTGGACGAGAGATTCCCAGGATGCATGCAGGGCCGCATTATGTATGTGC TTCCATTCAGCATGGGTCCCGTGGGCTCCCCACTCTCCCGCATTGGAGTGCAGCTCACTGACTCGGCTTA CGTGGTGGCCAGTATGCGTATTATGACCCGCTTGGGGACACCTGTACTTCAGGCCCTGGGAGATGGTGAC TTCATCAAGTGTCTGCATTCAGTGGGCCAGCCCCTGACTGGACATGGGGATCCTGTGGGCCAGTGGCCGT GCAATCCAGAAAAAACCCTGATTGGCCACGTGCCAGACCAGCGGGAGATCGTCTCCTTCGGCAGCGGCTA TGGTGGTAACTCCTTGCTGGGCAAGAAGTGCTTTGCCCTGCGCATCGCCTCTCGCCTGGCCAGGGATGAG GGCTGGCTGGCAGAGCACATGCTGATTTTGGGCATCACCAACCCTGCAGGGAAAAAGCGCTACGTGGCAG CTGCTTTCCCCAGTGCCTGTGGCAAGACCAATCTGGCCATGATGAGACCTGCATTGCCGGGCTGGAAAGT GGAGTGTGTGGGGGATGACATCGCCTGGATGAGGTTTGACAGTGAAGGTCAACTCCGGGCCATCAACCCT GAGAATGGCTTCTTTGGGGTGGCCCCTGGTACCTCTGCTGCCACCAATCCCAATGCCATGGCCACAATTC AGAGTAATACTCTCTTCACCAACGTGGCTGAGACCAGTGATGGCGGTGTGTACTGGGAAGGCATTGACCA GCCTCTTCCGCCTGGTGTCACCATAACCTCGTGGCTGGGAAAGCCCTGGAAACCTGGTGACAAGGAACCC TGTGCACATCCAAACTCGCGCTTTTGTGTCCCAGCTCGCCAGTGCCCCATCATGGACCCAGCCTGGGAAG CACCAGAAGGTGTCCCTATTGATGCCATCATCTTTGGAGGCCGCCGACCCAAAGGGGTACCACTGGTGTA CGAGGCCTTCAACTGGCGTCATGGGGTGTTTGTAGGTAGCGCCATGCGCTCTGAGTCCACTGCCGCTGCG GAGCACAAAGGAAAGACCATTATGCATGATCCCTTTGCCATGCGGCCTTTTTTTGGCTATAACTTTGGAC GCTACCTGGAACACTGGTTGAGCATGGAGGGACAAAAAGGTGCCCGGCTGCCTCGTATCTTCCATGTCAA TTGGTTCCGGAGAGATGAAGCAGGCTGCTTCCTGTGGCCAGGCTTTGGAGAGAATGCTCGTGTGCTAGAC TGGATCTGCCGAAGATTAGAAGGAGAAGACAGTGCCCAAGAGACTCCCATTGGGCTAGTACCAAAGGAAG GAGCCCTGGACCTCAGTGGCCTCAGCGCAGTGGACACCAGTCAGCTGTTCTCCATCCCCAAGGACTTCTG GGAGCAGGAGGTTCGTGATATTCGGGGCTACCTGACAGAGCAAGTCAACCAGGACCTGCCCAAGGAGGTG TTGGCTGAGCTCGAGGCTCTGGAAGGACGTGTACAAAAAATGTGACCTGAGGCTCTAGGCTAGCAAGAGC ACAGCTCCCCCATTTGGGTAGAGAACCCCCAGGCTCACAGAAAACATGAACAATCTGACATTAAAATGTG TGAGTGTTGGAAGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3596227 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3596227 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11500 same embryo
	EMAGE:11499 same embryo
	EMAGE:11498 same embryo
	EMAGE:11497 same embryo
	EurExpress:euxassay_006513 same experiment
	MGI:4827094 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source