EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11498

Pcyt2 phosphate cytidylyltransferase 2, ethanolamine ( MGI:1915921)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006514. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006514_01	euxassay_006514_02	euxassay_006514_03	euxassay_006514_04

euxassay_006514_05	euxassay_006514_06	euxassay_006514_07	euxassay_006514_08	euxassay_006514_09

euxassay_006514_10	euxassay_006514_11	euxassay_006514_12	euxassay_006514_13	euxassay_006514_14

euxassay_006514_15	euxassay_006514_16	euxassay_006514_17	euxassay_006514_18	euxassay_006514_19

euxassay_006514_20	euxassay_006514_21	euxassay_006514_22	euxassay_006514_23	euxassay_006514_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11498_wholemount_strong.wlz

11498_wholemount_moderate.wlz

11498_wholemount_weak.wlz

11498_wholemount_possible.wlz

11498_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11498_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6779
Entity Detected:	Pcyt2, phosphate cytidylyltransferase 2, ethanolamine ( MGI:1915921)
Sequence:	sense strand is shown >T6779 GCGGGAGCCGCCAGGATTTGCGGGGGGCCTCCGGTGCCATGATCCGGAACGGGCACGGGGCTGCCAGCGC TGCTGGGCTGAAGGGTCCGGGAGATCAGCGCATCGTGCGGGTGTGGTGCGATGGCTGCTATGACATGGTG CATTATGGCCACTCCAACCAGCTACGGCAGGCACGGGCCATGGGGGACTACCTCATCGTGGGTGTGCATA CGGATGAGGAGATTGCCAAGCATAAGGGGCCCCCGGTGTTTACCCAGGAGGAGAGGTACAAGATGGTACA GGCCATCAAGTGGGTGGATGAGGTGGTGCCCGCTGCTCCCTACGTCACCACCCTGGAGACACTGGACAAG CACAACTGTGACTTCTGTGTTCACGGCAATGACATCACGCTGACAGTAGACGGCCGAGACACCTATGAGG AAGTGAAGCAGGCTGGGAGGTACAGAGAGTGCAAACGCACCCAGGGTGTGTCCACCACAGACCTCGTGGG TCGCATGCTGCTGGTGACCAAGGCCCATCACAGCAGCCAGGAGATGTCCTCTGAGTACCGGGAATATGCT GACAGTTTTGGAAAGCCCCCTCACCCGACACCTGCCGGGGACACACTTTCCTCAGAAGTCTCCTCCCAGT GCCCTGGGGGGCAGAGCCCCTGGACAGGGGTGTCCCAGTTTCTACAGACATCCCAGAAGATCATCCAGTT TGCTTCTGGGAAGGAGCCCCAGCCCGGGGAGACGGTCATCTATGTGGCTGGTGCCTTTGACCTGTTCCAC ATCGGGCACGTGGACTTCCTACAGGAGGTGCACAAGCTAGCCAAGAGGCCCTACGTCATCGCCGGCCTAC ACTTTGACCAGGAAGTAAACCGGTACAAGGGCAAGAACTACCCCATCATGAACCTGCACGAGCGGACTCT CAGTGTGCTGGCCTGCCGGTATGTTTCAGAAGTGGTGATTGGGGCACCATACTCCGTGACAGCGGAGCTC CTGAATCACTTCAAGGTGGACCTGGTGTGTCACGGGAAGACAGAGATTGTACCCGACAGGGATGGGTCTG ACCCCTACCAGGAGCCCAAGAGAAGAGGCATCTTCTATCAGATTGACAGTGGCAGTGACCTCACTACAGA CCTGATTGTGCAGAGGATCATCAAGAACAGGCTGGAGTATGAAGCACGGAATCAGAAGAAAGAAGCCAAG GAATTAGCCTTTCTGGAGGCCACGAAGCAGCAGGAGGCGCCGCCTGGAGGGGAGATTGACTAGCTTCAGA CCCGGAGATGTTGTTCACGCCCTTGCCCTGTGCCCACCTCTTCTCTTCCTGCCCGGCTCTGCTTCTGTGT CTTGATGTCAGCTCACACAATTCCAAAGGAAACTGGCCTTGCTGAGGGTGCTGCCTGCCTGCGAGGCACC TGCCTTGCAGCAGGCTCTCAGCCCTTCCCTCTGAGCTGCTCAGAGAGGGTAGCTAGCCCAATGATGTGGC CCGTGGACAGGATAAGCAGATGCACCTGTGACTGACTGGAGGCGTGTTGCTGTGTATCCCGGGCATCCAT GGGTTCCAACTGCCACTGCCCCAGTCTTGGCCAGAGATACCCCTCCTGCCTGACTGGAAGCTGCACATCT GCCCACTGAGCTTTGGTGAAAGGTCCAGAGGCTTTGGGGACCTCTGTTCCTGGGCCACCCTGCCCGTGGG CACCCTCTACCTTGGGGCACGTTCTAGCACCCCATTCCTGACTCCTGGAAGATGCACTTGCCCCGACAGC TGGGCAGCACGGCTGTCCTCTGCAGAGACTGCCTGGTCCTCATTGTACTTTGGTGGCTCAACTGAATAAA GCCTTGTGGGAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3707732 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3707732 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11500 same embryo
	EMAGE:11499 same embryo
	EMAGE:11496 same embryo
	EMAGE:11497 same embryo
	EurExpress:euxassay_006514 same experiment
	MGI:4827109 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source