EMAGE mouse gene expression spatial database

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EMAGE:11501

Stc2 stanniocalcin 2 ( MGI:1316731)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003589. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003589_01	euxassay_003589_02	euxassay_003589_03	euxassay_003589_04

euxassay_003589_05	euxassay_003589_06	euxassay_003589_07	euxassay_003589_08	euxassay_003589_09

euxassay_003589_10	euxassay_003589_11	euxassay_003589_12	euxassay_003589_13	euxassay_003589_14

euxassay_003589_15	euxassay_003589_16	euxassay_003589_17	euxassay_003589_18	euxassay_003589_19

euxassay_003589_20	euxassay_003589_21	euxassay_003589_22	euxassay_003589_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11501_wholemount_strong.wlz

11501_wholemount_moderate.wlz

11501_wholemount_weak.wlz

11501_wholemount_possible.wlz

11501_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11501_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 06

head mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 07 21 22 23

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

thymus primordium

moderate

homogeneous

moderate expression: see section 13 15 16 weak expression: see section 12 14

pancreas

weak

regional

weak expression: see section 10 11 12 13 16 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 12 15 16 17 18 weak expression: see section 11

tongue

weak

regional

weak expression: see section 16

tongue muscle

weak

regional

weak expression: see section 11 12 13 14 15 16

testis

moderate

regional

moderate expression: see section 06 07 08 09 19 20 21 22

tail mesenchyme

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6723
Entity Detected:	Stc2, stanniocalcin 2 ( MGI:1316731)
Sequence:	sense strand is shown >T6723 GTCTCCAGGCGGGAGGGTGATACCCAGAACCATGTGTGCGGAGCGGCTGGGCCAGTTTGTGACCCTGGCT TTGGTGTTTGCCACCTTGGACCCGGCGCAGGGGACGGACTCCACGAACCCTCCGGAAGGTCCCCAAGACA GGAGCTCGCAGCAGAAAGGCCGTCTGTCCCTGCAGAACACAGCGGAGATCCAGCACTGTTTGGTCAATGC CGGGGACGTGGGCTGTGGTGTGTTTGAGTGTTTCGAGAACAACTCTTGTGAAATCCAGGGTTTACATGGG ATTTGCATGACGTTTCTGCACAACGCTGGAAAATTCGATGCCCAGGGAAAGTCATTCATCAAGGATGCCC TGAGGTGCAAGGCCCATGCCCTGCGGCATAAATTTGGCTGCATCAGCAGGAAGTGTCCAGCAATTAGGGA AATGGTTTTCCAGTTGCAGAGGGAATGCTATCTGAAGCATGACCTGTGCTCCGCAGCCCAGGAGAACGTC GGTGTGATTGTGGAGATGATTCATTTCAAGGATCTCCTGCTGCATGAGCCCTATGTGGACCTTGTGAACC TGCTGCTGACCTGCGGGGAAGATGTGAAGGAGGCAGTCACCCGCAGCGTCCAGGCTCAGTGTGAACAGAG CTGGGGAGGCCTCTGCTCCATCCTGAGTTTCTGCACCTCCAACATACAGAGACCTCCCACGGCAGCCCCA GAGCATCAGCCCCTGGCAGACAGGGCTCAGCTCTCCAGGCCTCACCACCGGGACACAGACCATCACCTAA CAGCCAACAGAGGTGCCAAGGGTGAGCGAGGGAGCAAAAGCCACCCGAATGCCCATGCTCGAGGCAGAAC CGGTGGCCAGAGCGCTCAGGGACCCTCTGGAAGCAGTGAGTGGGAAGATGAACAGTCTGAGTATTCCGAC ATCCGGAGGTGAAATGAAAACCGGGCCATGAAAGCTTTCCTCCAGGCTGTCCATTTTCTTATCTATGGAC ATTCCAAAACATTTACATTAAAGAGGGGGGATGTCACACGCTGGATGTGTGCAAATTGTGGACTTCGTGC AGTTGTGTGAACAGGTGAGATGAAGATTCCCGGGCAGCAAGGTCTCGAGTGCCTGGTGGGTTGGCACCTG TGTAAGCTTATTGGCGGGAGTGTTGTATTGAATCGGCCGTGTAACTGTCTCTTCGGTCTGTGGAGCTAGT TGGTGGCTTAAGTATGGTCTGTTGGGGGGAGGTCAGCCGGGGAGGGAAGGGGCCACAGGTGGGTGGTAAA CCCTCCCCCACAGTTCCCAGTACTGTCTGCACAGAGCAGGGGCTGGGTTCTGGGGTAGGGCTGAGTGGAG ATACAGCTGACAAGGCAGAGAGAGCAGAGAGGAGGAAGGGCTTAGCCCCACTCAGTCCCAAACCGGAAGT TTAGTGTCGTGCATGGGATCTAGTGTTTCAGAGCTGCTGGAGGGGACATGGATGGTACATTTTCTGTGTG TCATTTCTGAGCCATTGTTCTGTCTGGGGGACACTGAAGGAGTGGCCCTGTTTGTTTATTTTTGACCACT GTTTCAAGTCTCCATTTTACTTTTTTTTTTTTAAATCAACTGACATCTATGTGTCTGTCATCTAAATAAA TGGCTTTCAAACCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3665803 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3665803 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11502 same embryo
	EMAGE:11505 same embryo
	EMAGE:11503 same embryo
	EMAGE:11504 same embryo
	EurExpress:euxassay_003589 same experiment
	MGI:4828495 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source