EMAGE mouse gene expression spatial database

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EMAGE:11504

Sh2b3 SH2B adaptor protein 3 ( MGI:893598)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003586. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003586_01	euxassay_003586_02	euxassay_003586_03	euxassay_003586_04

euxassay_003586_05	euxassay_003586_06	euxassay_003586_07	euxassay_003586_08	euxassay_003586_09

euxassay_003586_10	euxassay_003586_11	euxassay_003586_12	euxassay_003586_13	euxassay_003586_14

euxassay_003586_15	euxassay_003586_16	euxassay_003586_17

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11504_wholemount_strong.wlz

11504_wholemount_moderate.wlz

11504_wholemount_weak.wlz

11504_wholemount_possible.wlz

11504_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11504_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw molar

moderate

regional

moderate expression: see section 07 08 16

upper jaw molar

moderate

regional

moderate expression: see section 07 08 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6804
Entity Detected:	Sh2b3, SH2B adaptor protein 3 ( MGI:893598)
Sequence:	sense strand is shown >T6804 GATGGGCTCGGGCCGTCGCCTCCGCCTGGCCGCTCTCCAGCCCAGACTGTCACGGGCCACACGGGGTCCA CATCGGCGGCGGCAGCAGCAGCAGCGTCCTGGGGACGGCCCGAGGCCGCGTCCCCCGGCTGCTGTCAGGA GCTGGCGGCGGAAATGAGGCGCTGGCCGTTTTCCGAGCCGGGGTTTCCTGCCTGAGCGCCGAGAGGCCGT GCGACGCCATGGGCCGGCCGGGCCCCGACCCGCAGCTTGCGCGCCCGGCCGCCCCGCCTCGCTCTCCACC ATGAACGAGCCCACCGTGCAGCCGTCCCGCACATCCTCCGCACCCGCCTCGCCGGCATCCCCACGCGGCT GGAGCGACTTCTGCGAGCAGCACGCAGCAGCGGCGGCCCGGGAGCTGGCCCGCCAGTACTGGTTGTTTGC GCGCGCGCACCCACAGCCGCCGCGCGCGGACCTGGTGTCGCTGCAGTTCGCGGAGCTCTTCCAGCGCCAC TTCTGCCGGGAGGTGCGCGAGAGCCTCGCAGGACCGCCGGGTCACGACTACCGCGCCACTGCTCCGCCCC GCCCCGCGCTGCCCAAGGCACGCAGCTCCGAGGACCTGGGCCCGCGGCCCGCCTGTGCCCTGCAGCACCT GCGCCGCGGCCTGCGCCAGCTCTTCCGCCGCCGCTCGGCAGGGGAGCTGCCCGGGGCTACCAGTGACACC AATGACATCGACACCACCGCAGCCAGCAGGCCGGGCCCGGCCCGCAAGTTGCTACCCTGGGGCCTGCGAG AGCCGCCCACTGAGGCGCTCAAGGAGGTCGTATTGCGCTATAGCCTGGCGGACGAGGCAGCAATGGACAG CGGCGCACGCTGGCAGCGGGGTCGCCTGGTGCTTCGGTCTCCAGGTCCGGGCCACAGCCACTTTCTGCAG CTCTTCGATCCGCCCAAGAGCTCAAAGCCCAAGCTCCAAGAGGCCTGTTCCAGCATCCGGGAGGTCCGAC CATGTACACGCCTGGAGATGCCTGACAACCTCTACACCTTTGTGTTGAAGGTGCAGGACCAGACAGACAT CATCTTTGAGGTGGGAGATGAACAGCAGCTGAACTCATGGCTGGCAGAGCTCAGGGCAAGCACAGGCCTT GGGCTGGAGCACCCGGACACCGAGTTACCTCTTTCCTTAGCGGCAGAGCCTGGCCCAGCTAGATCCCCAA GGGGAAGCACTGACTCCCTGGACCAAGGTGCTTCACCTGGGGTGTTGCTGGACCCAGCCTGCCAGAAAAC AGATCACTTCCTATCCTGCTACCCCTGGTTCCACGGCCCCATCTCCAGGGTGAGGGCTGCACAGCTGGTC CAGCTCCAGGGCCCTGATGCCCACGGCGTGTTCCTGGTGCGGCAGAGTGAGTCCCGGAGAGGAGAGTATG TACTCACATTCAACTTACAGGGCAGAGCCAAGCACTTACGCCTGGTGCTCACAGAGCGTGGACAGTGCCG GGTGCAACACCTGCACTTCCCCTCGGTGGTAGATATGCTCCGCCACTTCCAGCGTTCTCCTATCCCACTG GAATGTGGAGCAGCTTGTGACGTCCGACTCTCTGGCTATGTGGTAGTCCTCTCTCAGGCACCAGGTTCCT CCAACACCGTCCTCTTCCCTTTTTCCCTTCCTCACTGGGATTCGGAGCTGGGTCATCCCCACCTCAGCTC TGTTGGCTGTCCCCCCAGCCATGGTGCAGAGGCTCTCCCTGGCCAAGTGACACCACCTGAGCAGATCTTC CACCTGGTGCCTTCTCCTGAGGAACTGGCCAACAGTCTGCGGCAGCTGGAGCTCGAGTCTGTGAGCAGTG CCCGGGACTCGGACTATGACATGGACTCCTCTTCACGGGGCCACCTTCGGGCCATTGACAACCAGTACAC CCCTCTCTCACAGCTGTGCAGAGAGGCAGACGTGTGAATGGAACCATTTTCCTCCCCTCCAGAGAACTAT AGGCTGCTGTCAGCCTTCCCCAGCTCAGATGTGCCCCGCTTCCAACACACACAAGGCTGTCACAGGCTTC CTGTCAGCCAGAGCTAGCCCCCGGCTTTCTCCCACTGTTTACAGATGTAGTTCTTGTGCACAGGTGCCAC TAGCTGGTACCCTAGGCCTAGTCCCCAACCCAAAAGGAGGTGGGGCTACAGGCCCAGAGCTGGCAGTGGA AACTGACAGAGCTGATGACAGACTTCTTACCAGGCAGGGTCATCGGGAAGCACAAAACACTAACAGTCCC TGGATTCTCACATGGTGTCCTTCCCTGTAGCTTCAGATCTGTGGCAAGTCCTCATGGGTTCCATGCCACA GGCGATGGATGCTTCATGATCCTGCTCCTACTCTTAGCTTTACGACAGAAGCTCTGCCAGAGGGACAAGC TTAAAGATCAAAATTGATTTAAACATTTTACCTCAGAGTACTTTTTTTTGTTTTGTTTTAAAGGATTCAG GTTTCCATTTAATATTAAAGCTTATTCACTGCAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3709861 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3709861 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11502 same embryo
	EMAGE:11505 same embryo
	EMAGE:11503 same embryo
	EMAGE:11501 same embryo
	EurExpress:euxassay_003586 same experiment
	MGI:4828032 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source