EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11518

Lsm2 LSM2 homolog, U6 small nuclear RNA associated (S. cerevisiae) ( MGI:90676)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006961. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006961_01	euxassay_006961_02	euxassay_006961_03	euxassay_006961_04

euxassay_006961_05	euxassay_006961_06	euxassay_006961_07	euxassay_006961_08	euxassay_006961_09

euxassay_006961_10	euxassay_006961_11	euxassay_006961_12	euxassay_006961_13	euxassay_006961_14

euxassay_006961_15	euxassay_006961_16	euxassay_006961_17	euxassay_006961_18	euxassay_006961_19

euxassay_006961_20	euxassay_006961_21	euxassay_006961_22	euxassay_006961_23	euxassay_006961_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11518_wholemount_strong.wlz

11518_wholemount_moderate.wlz

11518_wholemount_weak.wlz

11518_wholemount_possible.wlz

11518_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11518_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9905
Entity Detected:	Lsm2, LSM2 homolog, U6 small nuclear RNA associated (S. cerevisiae) ( MGI:90676)
Sequence:	sense strand is shown >T9905 GAACCGTGCGGGCGTGGGTGCGGGCACTCGTGTCGGTCGCTTGGGGCGGCAGCCAGGCCCCAGGGTTTTC CGTACAGCCTTACTGCCCACACCCCAGTGTGAGGAGGCTGCTCGGAGCCGATGGTGTTTTGGGGGAAACC TTTAGCAAGTCCAAGAGAAAGCCCACGGGCGCCGGGGGAGAACGGGAAGCGAGCCTTGCACTTCTGTGCT GTTGACGAGGACTTTGTGAGGCTCTTCTACTCCTTTTTCAAGTCTCTTGTGGGCAAGGATGTAGTCGTGG AACTCAAGAATGACCTGAGCATCTGTGGAACCCTCCACTCTGTGGACCAGTACCTCAATATCAAACTAAC CGACATCAGTGTCACAGACCCTGAGAAATACCCTCACATGTTATCAGTCAAGAACTGCTTCATCAGGGGC TCAGTCGTCCGCTATGTGCAGCTGCCAGCAGATGAGGTGGACACACAACTGCTACAGGATGCAGCAAGGA AGGAAGCCCTTCAGCAGAAGCAGTGATGGCACCTCCCCCCTCCCCCTCACTTCCCCATTGGTGACCCAGA ACTTGACGTCCTACTGAGGACAAGAACACCCCTGCCCAATACTTAAGGTGTGTTTTGTTTTGTTAATGAT TTTTTTTTTTTTCTTTTTAACTGAATGGATGAGAGATAATTCCTCTGCTGAGAAGGGAGGGTAAGAGAGC CAGGAACTGCAGAATGGTCCTGGACTGGCTCCCAGTCTGTACCTTTTTCTCATCTTTCCTGGAGATGGGA GCAGCATCCTAAGCCCTTCCGTGGCGACATGTAATACATTTGAGGGATGGAAAGATCTGTCCCGCATCGG GAATAAAACTTATGATGCAAGCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6774564 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6774564 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11519 same embryo
	EurExpress:euxassay_006961 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source