EMAGE mouse gene expression spatial database

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EMAGE:11527

Rbm12b RNA binding motif protein 12B ( MGI:1919647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006812. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006812_01	euxassay_006812_02	euxassay_006812_03	euxassay_006812_04

euxassay_006812_05	euxassay_006812_06	euxassay_006812_07	euxassay_006812_08	euxassay_006812_09

euxassay_006812_10	euxassay_006812_11	euxassay_006812_12	euxassay_006812_13	euxassay_006812_14

euxassay_006812_15	euxassay_006812_16	euxassay_006812_17	euxassay_006812_18	euxassay_006812_19

euxassay_006812_20	euxassay_006812_21	euxassay_006812_22	euxassay_006812_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11527_wholemount_strong.wlz

11527_wholemount_moderate.wlz

11527_wholemount_weak.wlz

11527_wholemount_possible.wlz

11527_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11527_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 14 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7916
Entity Detected:	Rbm12b, RNA binding motif protein 12B ( MGI:1919647)
Sequence:	sense strand is shown >T7916 CCGTGTTGGGCCTGGTAGGCCGTGCGGAGACTGCGGCGTCGAGGAGCGTCCCTTCTCCGTTTGTGTGGTG TGTGGTCACTGCCCGGTGCGAAGCACTTCTCAGGATGCCGGGCATGGGGCAAGGGAGGGGGTGAGAGCCG GAAGCACGGGCGTGCTGGGCAGGGGGCGAGCGCGGCTGGTGCGGTTAGGGCGGCGCTCGCTGACCCCCCG ACTTCCTCCTGTCAGCCTCCGTGTGGCGGCGAGTGAGCTTCCTGGGACCTTGACAGCGAAAATCTCGGAG CAGCGCCTAGTTAAGTTGCTTTTACAGAATAAACAGGTCTCCAAGAAATAAAAGGCAAAGGTCATTATTG CTGTGGCTTGAGCTCAGCATGGCTGTAGTCATCCGTTTACTGGGGCTTCCTTTTATTGCGGGTCCTGTGG ATATCCGTCACTTCTTCAAGGGATTGACTATTCCTGATGGAGGAGTGCATATAATTGGAGGGAAAGTTGG GGAGGCTTTTATAATTTTTGCAACAGATGAAGATGCAAGACGTGCTATAAGTCGCTCAGGAGGGTTTATC AAGGATTCATCTGTAGAGCTTTTTCTTAGTAGCAAGGTAGAAATGCAGAAGACCATAGAAATGAAAAGAA CTGCTCGAGTAGGAAGAGGGCGACCAGGATCCGGGGCATCAGGGGTTGGCAACGTGTATCATTTTAGTGA TGCTCTGAAGGAAGAAGAAAGTTATTCTGGATATGGCTCTTCGGTTAATCGAGATGCTGGGTTTCATACA AATGGTACAGGACTTGACTTAAGGCCAAGAAAGACCAGGCCATTGAAGGCTGAGAATCCTTACTTATTTC TACGAGGTTTGCCTTACTTAGTAAATGAAGATGATGTCCGTGTCTTTTTCTCTGGTTTGTGTGTAGATGG AGTAATTCTCTTAAAACATCATGATGGCCGAAATAATGGTGATGCTATAGTAAAATTTGCTTCATGTGTT GATGCTTCAGGAGGTCTTAAATGCCATAGAAGTTTCATGGGTTCAAGATTTATAGAAGTCATGCAGGGTT CAGAACAACAGTGGATTGAATTTGGTGGTACTGCAACTGAGGGCGGTGACACTCCTCGTATGAGATCTGA AGAGCATTCTCCTTCAAGAAGGATTAATGGTAGACATTTTCGGAAACGGTCTCATTCAAAATCTCCTAGA GCACGTTCTCGCTCTCCTCTTGGATTTTATGTTCACTTAAAAAATCTGTCCTTAAATACTAACAAAAGGG ATTTAAGGAATCTCTTTAGAGATACTGACCTGACTAATGACCAGATTAAGTTTGTGTATAAAGATGAAAG AAGAACACGCTATGCCTTTGTGATGTTCAAGAATCAAAAAGACTATAATACTGCCCTGGGTTTGCATAAG ACTGTTTTACAATATCGTCCAGTCCTTATTGATCCAGTGTCTAGGAAGGAAATGGTGAGAATCATTGAAT GCTATGAGAAGAAGAGACCAGAGTCTTTAGAGAAGGAAAGGCCAGGACGTGTTTCACAAAAGTACTCTCA AGAAGGCTTCTCTGGCTCTGGTCAGAAGCTGTGCATATATATAAGAAATTTACCATTTGATGTTACAAAG GGTGAAGTGCAGAAGTTCTTTGCAGACTTTTCTCTTGTTGAGGATGACATTTATCTGCTTTGTGATGACA AGGGAGTTGGTTTGGGAGAAGCACTGGTGAGATTTAAATCAGAAGAACAAGCCATGAAAGCTGAACGTTT GAACCGACAAAGATTCTTGGGGATAGAGGTGTTGTTAAGACTTATATCTGAGGAACAAATGCAGGAGTTT GGTGTAAAGTCTTCCTGGTTGTCTAATGAGAGGACACAAGCGTGTTCCAGGTCACACGATGGAGATGACT GTTCCTGTTTGTTTGACTTAAAAGATCCGTCATCCTGTTCATTTGGCCAGTCTGAAAGCCTTCGTTACCA TCCAAAAGACTTAAGGAAAATGGGTCATTTCAAGCATCCCCAAGGGTATTTCCGGCAGTCTGACAGGCGC TCTCCTGAGGACTTCAGGCACTCCCCAGAAGACTATAGGCACCCCTGGGAGGAGCATACCAGCCACTCTA GGGAGGAAGACTGGAGACTGCCTCTGGAGGATTGGAAATGGCCACAGGAAGATGATTTTAGGCAGTGTCA TGAGAAAGATCATAGGCAGCTAAGAAGTCCTTGGGAAGAAGACTTCAGACGGCCATCTCAGGAGCACTTC AGGAGATCCTATCAGGAGCACATTAGACAGCCACCCCAAGAACATTTCAGGCGTTCCCGAGAGGAAGATT TCAGGCATGTGGCTGATGAAGACTTTAGGCAAGCTTCTGATGAGGACTTCCGGACCTCCCAAGAAGATTT GAGATATCCCACTGATGAGGACTTTAGGAGGGTGTCTGTGGAAGACCTCAGGGAAGTTCCAGAGAAGGAC CTCCGACTTCCTAAAAATTTTAGATCTCCTGGAGAGGAGTTTTGGACCCCACCTGATTTCAGAGGTCAAC ATCCCTTTGGGAACTTTGATCATCTACAAGGTGGCAAATTTGATTTTGAAAAGTATAAATTTGGAAGTTT TCCTGATGCAAAAGTTACCTCTGATCTAAATTTAAATTGTGTTTCAGATAAAATAATTCCTGTTAAGATA TCCAATCTTCCATTCAAAGCTAATGCTAGTGAAATTCTAGACTTTTTTCATGGTTATAAAGTCATACCTG ATTCCGTTTCATTACAGTATAATGAGCAAGGATTACCTATAGGAGAAGCCATTGTTGCTATGACAAATTA TAATGAAGCTCTAGCTGCAGTTAAAGATCTAAGTGGTAGACCAGTTGGTCCCCGCAAAGTTAAGCTTAGT TTGCTTTAGAGGGCCTTTCTAAGTAAACAGTTTTCTTCCACAGTGTTCATGCAGTGAAAGTAAAGTTTTT TATAGAGATGAAAAATTTTATTTTTAACTTGTGAATAGAAAAAATGTGTTATGTAAATCTCGTTTCTTTT TGCTTATAAATGGACATTCTTTTACCCCCTAATTAAAAATAAAAAATATTCTGTTCTTGAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6513134 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6513134 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11528 same embryo
	EurExpress:euxassay_006812 same experiment
	MGI:4827648 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source