EMAGE mouse gene expression spatial database

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EMAGE:11546

Gpd1 glycerol-3-phosphate dehydrogenase 1 (soluble) ( MGI:95679)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006861. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006861_01	euxassay_006861_02	euxassay_006861_03	euxassay_006861_04

euxassay_006861_05	euxassay_006861_06	euxassay_006861_07	euxassay_006861_08	euxassay_006861_09

euxassay_006861_10	euxassay_006861_11	euxassay_006861_12	euxassay_006861_13	euxassay_006861_14

euxassay_006861_15	euxassay_006861_16	euxassay_006861_17	euxassay_006861_18	euxassay_006861_19

euxassay_006861_20	euxassay_006861_21	euxassay_006861_22	euxassay_006861_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11546_wholemount_strong.wlz

11546_wholemount_moderate.wlz

11546_wholemount_weak.wlz

11546_wholemount_possible.wlz

11546_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11546_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 04 05 06 07 08 20 22

thyroid gland

moderate

regional

moderate expression: see section 10 11 15 16

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 10 11 13 14

pons mantle layer

strong

single cell

strong expression: see section 11 moderate expression: see section 13 not examined expression: see section 16

pons ventricular layer

strong

regional

strong expression: see section 09 10 14 15 16

midbrain mantle layer

strong

regional

strong expression: see section 11 14 moderate expression: see section 13 weak expression: see section 08 not examined expression: see section 16

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 14 15 16

spinal cord ventricular layer

strong

single cell

strong expression: see section 11 12 13 14 15 16

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22 23

kidney calyx

strong

regional

strong expression: see section 09 moderate expression: see section 10 21 weak expression: see section 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7401
Entity Detected:	Gpd1, glycerol-3-phosphate dehydrogenase 1 (soluble) ( MGI:95679)
Sequence:	sense strand is shown >T7401 GCAGCAAGCAGCACCATGGCTGGCAAGAAAGTCTGCATTGTCGGCTCTGGCAACTGGGGCTCAGCCATTG CCAAGATCGTGGGTAGCAATGCAGGCCGGCTGGCACACTTTGACCCACGGGTGACCATGTGGGTGTTTGA GGAAGACATCGGGGGCAGAAAGCTAACTGAGATCATCAACACTCAGCACGAGAATGTTAAATACCTGCCA GGGCACAAGCTGCCCCCCAATGTGGTGGCCATCCCAGACGTGGTCCAGGCTGCAACAGGCGCTGACATCC TGGTTTTTGTGGTACCCCATCAGTTCATTGGCAAGATCTGTGACCAGCTCAAGGGCCACTTGAAGGCCAA TACTATTGGCATATCTCTTATTAAGGGGGTAGACGAGGGCCCCAACGGGCTGAAGCTCATTTCTGAAGTG ATTGGGGAGCGCCTTGGCATTCCCATGAGCGTGCTGATGGGGGCCAACATTGCCAGCGAGGTGGCTGAGG AGAAGTTCTGTGAGACGACCATCGGCTGCAAGGACCCGGCCCAGGGACAGCTCCTGAAGGACCTGATGCA GACACCCAACTTTCGCATCACTGTGGTACAAGAGGTGGACACAGTGGAGATCTGTGGGGCCTTGAAGAAT ATAGTGGCCGTTGGGGCTGGCTTCTGTGATGGGCTTGGCTTCGGTGACAACACCAAGGCGGCGGTGATCC GGTTGGGGCTCATGGAGATGATCGCCTTCGCCAAGCTCTTCTGCAGTGGCACTGTGTCCTCGGCCACCTT CCTGGAGAGCTGCGGGGTCGCAGACCTCATCACGACCTGCTATGGGGGGCGGAACCGCAAGGTGGCAGAG GCCTTTGCTCGAACTGGAAAGTCCATTGAGCAGCTGGAGAAGGAGATGCTAAATGGGCAGAAGCTACAGG GGCCCCAGACAGCCCGGGAGCTGCACAGCATTCTCCAACACAAGGGCCTTGTGGACAAGTTCCCCTTGTT CACTGCGGTGTACAAAGTGTGCTATGAGGGCCAGCCAGTGGGCGAATTCATCCGCTGTCTGCAGAACCAC CCGGAACACATGTGAATGGGGCCAGGGCCCAGGACAGGCAGCCCCTTTGCCCCAACGGAGACAAGCGGAA GGAAGCACGTGACACCCGTCATCAGGACTGTCTCCAGGACTCCCCATCTAGCAGAGTCCTCTCTGAAGGA CCCTGAGGACAGGAGGCTGCGGGGCTCAGCTACACACCTAGAGATGCTCGCCACAGAATCCACACTTCCT TGCTGTCCTCTGGGAAGTGTAGAACTAAGCCCCAGTGGTGCCTGCTCCAGGGGTGGGGTTGGGGGAAGGG AAGGCGCCAAGTCAAGGGCTGCCGGTTGCTGCCTCACACACACTGGGAACCGGTCCCAAGTCCCATTAAG TGACTGAAGAAGCACTTCAGCCACAGGAAAGATGGGGCAAAGGCTGCCAAGGGAAGGGTCTGGATGCCTG AGCTGACACAGACCCCAGAGACCCTTTGGCCGACCTCTGCCAGGCCCCACACAACATCAACGATCTCACT GTTTGCTAACAAAATACAAAGCTCTCAAACAACCCCACTCTAACTTCTCCTAGCAACACTTGCTTCCTCA GAAGCCTCTACTTCCCCCTCCCCCAGGCTACTCTGGCACAAGAGTTGCTCTCCACAGGCATCTCTGGCCC TGGGGCGCTCCATTCTTCCCAGAGACTTCCTGGTTCCTGGAGCTTTGTCAGGGCCTCCCCACCCTGTGTG ACCTTTCCCAGCTTTCCCTTGCCTGCACCCAGCTGCCAGCACCCTCTTTGGGGAGCAGGAATCTAATCTG CTGACAGAGCTACACTCTTTTCACAGCAGGCTCAGATGGCTCAGGCTCCCCTGACCTTCCTATTTGTCCA CCTCCCCAGTCAGGAGCTCCGGCCCCGGCCAGCCCCTCCTACTGCTGACCTTTCTTCTCAGGGTCCTCCC CACAAGGCCGCTTCTCTCTGCAGTCACCACCAACTTCACGTCTAACAGAATGTAGGATTTATAGTTTATC GTCCTCAGGGCTGTCAGCCCATATTCTTGACTCTGCCCTTTCCTTTTGCCCAAGTCGTTCTCACCCAGCC TGGAAGGCCACCCGGCATCTGTAGGCTTAGAACTAAGGAGCAGCTCAAAGGTTCAGTAGGTTTCCACAAG GGTTGGTGGGTCCTAGTCCAAGGCCAGCTGTCCTCGGAACAGAACAAGGTCCTCGCGAGATCGGACCCAC ACACCTCAGGAGCTGGGGAACTCGAGGGAAGGACAATTGAAAACTGCCTGCCCTGGGGTGAGGGAGATCA GAGGGGGATGGGAGACTGCTTTGCACTGGCCCTGTTCCAAAGTTGGGTGGCAGCAAGAGAATGTCACAGA TCAGCAGCCTGGAGCGCTAGCTATAAATAGCTGGAGGTTTCTCTCTCCACCAGGGGTCTCCATTTCCTGT GTGGCAGCAACTGGGACACCGTGGCTCTGGCCAGCTTGATATTAAACTAACACATTAAGGTGCCAGGTCC CTCTCTGCTCCTAGCCTTTAAGAATTCCGTCTTAGTGAAGGTCAGAGCTGTCGGGTAAGGGGAAGAGGGG GGGCCCTGTCATCGATCCCGACTGGGCTGGAACCCTCCCACGCATGACTCAATTCAGAGCTGTTCCCCGG GAGACTGGGGCGGGATGCAGACAGATTCCAACACCTTAACCTATGTTTGCTCAGTCAGTCAACTGTGAAT CTGAGGTCTTCTGTCAGGCCACTTGTCTACCCAATAAAGTGTGGTTTTTCCAGAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4194849 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4194849 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11547 same embryo
	EMAGE:11549 same embryo
	EMAGE:11548 same embryo
	EMAGE:11550 same embryo
	EurExpress:euxassay_006861 same experiment
	MGI:4825159 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source