EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11550

Oxnad1 oxidoreductase NAD-binding domain containing 1 ( MGI:1916953)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006867. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006867_01	euxassay_006867_02	euxassay_006867_03	euxassay_006867_04

euxassay_006867_05	euxassay_006867_06	euxassay_006867_07	euxassay_006867_08	euxassay_006867_09

euxassay_006867_10	euxassay_006867_11	euxassay_006867_12	euxassay_006867_13	euxassay_006867_14

euxassay_006867_15	euxassay_006867_16	euxassay_006867_17	euxassay_006867_18	euxassay_006867_19

euxassay_006867_20	euxassay_006867_21	euxassay_006867_22	euxassay_006867_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11550_wholemount_strong.wlz

11550_wholemount_moderate.wlz

11550_wholemount_weak.wlz

11550_wholemount_possible.wlz

11550_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11550_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7119
Entity Detected:	Oxnad1, oxidoreductase NAD-binding domain containing 1 ( MGI:1916953)
Sequence:	sense strand is shown >T7119 ACAGCGTCCTCTCCAGTTCTCGACCCACAGCGCTCCTGCAATTGCGTTTCAGGCGACCTTGAACTCTGAG TGACTCTTGGCCTGTTATGAGTTCTTGCCTGCAGGAAGCACCATGGCCCACGTTGCCTTGATTCCTGGCT TGTCTCGAGGATCTGTGGGAGCCGTCTGCACTCAGGCTGCTTCGTGGGGACTGAAGGCCAGCACTTTGCG TCACCTTACCCTAGCCAGCATAATTAAATCCAGAAGGAAAACGGACCACCTAGAGAGAACCGCCAGTGTC CTCCGCCGGGAGGTCATGGCTGCAGCTAAGGTGTGTGAAATCACTCACGAGTCTCCATCAGTGAAGAGCC TCCGTTTGCTTGTTGCTGATAAAGACTTTTCCTTTAAGGCTGGCCAGTGGGTTGATTTCTTCATCCCAGG AGTCTCAGTGGTTGGTGGGTTTTCAATTTGCTCCAGTCCCCAACGGCTAGAACGAGACAGAATAATAGAA CTGGCGGTGAAATATGCAGACCACCCTCCTGCTGTCTGGGTTCACAATAAGTGCACACTTGACTCTGAAG TGGCTCTCAGAGTAGGTGGAGAGTTCTTCTTTGACCCTCAGCCCACAGATGCCCCTCGAAACCTCATCTT GATTGCTGGAGGGGTCGGGATTAACCCCCTGCTTTCTATCCTGCGCCACTCGGCAGATCTCCACCGGGAC CACGCAGACAAAGGAAGTGGCTATGAGATAGGGACAGTAAAACTGTTCTACAGTGCAAAGAACACCAGCG AGCTCCTGTTTAAGAAAGATATCCTCGACTTAGTACATGAATTCCCTGAGAAGATTTCTTGCAGTTTTCA TGTTACAAAACAGACAGCACAAATCAGTGTGGAACTGAAGCCTTATGTCACAGACGGAAGAATAACGGAG AAGGAGATAAGAGATCACATCTCAGCAGAGACTCTGTTCTATGTCTGTGGACCACCTCCAATGACAGACT TTTTCTCTAAGCACCTGGAAAGCTGCCACGTTCCCAAGGAACACATCTGCTTTGAGAAGTGGTGGTAGGG AACGGGCACAGAAGACCTGAGAGCTGCTTGGGACAGTGAGGCTCTCAAGTGTCCAGCCCGGCTACGCGAG TCACACGAGAAACTTCCGGCAGACTTCATTGGTCACACTCTTTGACTATATTGATTTTTCTTTCGTTAAT AGTAATTCCATTATTACAGCATTTCTGACCCCTGGGAAAAGACAATTCAGCTTTACTTAAAAGATGTATT GTTGAGCTGCTGGCATGGCAGGCTGACAGTCTGAGGGCCACATTTCAGATTGCCATAAAGGAGGGGGTGG GGAGAGGGTTCCTAAGGGAAGAACCACAGGAAGGCAGGCCCACAGGATCTACGCAAGCAAGCAGACACTG GCCTGGTCTGCCAAGTTTAAGTGGTTAAAGCAACTGAAAACAACCTGGGTATCCACATAAGCAAGTTACA GAAGTGAAGACGCAGTCTCCATACCAAGGAGCCACTCACAGCTGGACATTTCTGGGAGTTCCCTAGTCAG GGTTGTTTTCTGGGACGCCATCTTTCTGGAATTTTGAGTTTGTGGCAAGCTGTCATTAGTACTAACATGG AGGGGACCTAGAAAACCATGGGGTTTCTCTGGTTGGCCTCTACACCATGAACTGATTAATACAGAGTTTT CTTTCGCCTTTAGGGGACAAATTGGTCTACTTACATTAATTAAAGAAAATGTGGGTTTTGTGTGAACTGA ATACTTCTTTAAATTATTGAACATAAATTCATAAAATGTGTGTCTAGGGAATTTATAATTTTAAATACAG TCTTTATTTTGAAGGGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4018387 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4018387 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11547 same embryo
	EMAGE:11549 same embryo
	EMAGE:11546 same embryo
	EMAGE:11548 same embryo
	EurExpress:euxassay_006867 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source