Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11557

Dcpp1 demilune cell and parotid protein 1 ( MGI:105949)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11557 EMAGE:11557 EMAGE:11557 EMAGE:11557 EMAGE:11557
"Pseudo-wholemount" of euxassay_006745. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006745_01 euxassay_006745_02 euxassay_006745_03 euxassay_006745_04
EMAGE:11557 EMAGE:11557 EMAGE:11557 EMAGE:11557 EMAGE:11557
euxassay_006745_05 euxassay_006745_06 euxassay_006745_07 euxassay_006745_08 euxassay_006745_09
EMAGE:11557 EMAGE:11557 EMAGE:11557 EMAGE:11557 EMAGE:11557
euxassay_006745_10 euxassay_006745_11 euxassay_006745_12 euxassay_006745_13 euxassay_006745_14
EMAGE:11557 EMAGE:11557 EMAGE:11557 EMAGE:11557 EMAGE:11557
euxassay_006745_15 euxassay_006745_16 euxassay_006745_17 euxassay_006745_18 euxassay_006745_19
EMAGE:11557 EMAGE:11557
euxassay_006745_20 euxassay_006745_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11557Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11557_wholemount_strong.wlz
11557_wholemount_moderate.wlz
11557_wholemount_weak.wlz
11557_wholemount_possible.wlz
11557_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11557_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
strong strong
regionalstrong expression: see section 05 06 07 08 09 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7340
Entity Detected:Dcpp1, demilune cell and parotid protein 1 ( MGI:105949)
Sequence:sense strand is shown

>T7340
AAGGCGAATCCTCTCAGCCAGACTTTGAGATGTTCCAGCTGGAGGCCATGCTGCCCTTACTGATTCTTGC
CTTCTTGGGGACCCCCACGGTTTTGACACAAGATTATCATGGTCCAGAAGTTGGAAAACATTCCTGCACT
AGTGCACCTGAAGGAAAAAATATAACAAGTATCCGGGTATTTCTTCAGGGTAGATCGATTGTTGGAATTC
AGTTTAACTACAACAATGAGGACGGTCAAGTGTATGGCTCTACTGCTGGGAAGGTGATGGTAGCAAGACT
GAATAACGAAGAGTCCATCATTGCGGCCGAGGGCACATACTCTCCTTCGGCTTTAACTCAGATAATCTTT
ACGACCAACCAGCCTCGCCAGCTAATGGTGGGGTATTATGTGGGCAGTTCTGAGTACTCTAGCTTCCCTG
ACGATCCCAGCCATGTGCTCAAGGGAGCTTGTGTCTCTTGGAGGGCAGGTGGCATAAAGAGTATCTTGTT
TTTGTGGGGAACTGAAAACAGTTCATGTGTGAAATATGGCCACTCAGGCTGAAGTGGTCTGTGTCAGGTA
CTTCCAAACTGAACCCATGAATAAATACAACATCTGCAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4190591 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4190591 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11559 same embryo
 EMAGE:11558 same embryo
 EurExpress:euxassay_006745 same experiment
 MGI:4824252 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS