EMAGE mouse gene expression spatial database

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EMAGE:11561

Sez6l2 seizure related 6 homolog like 2 ( MGI:2385295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006780. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006780_01	euxassay_006780_02	euxassay_006780_03	euxassay_006780_04

euxassay_006780_05	euxassay_006780_06	euxassay_006780_07	euxassay_006780_08	euxassay_006780_09

euxassay_006780_10	euxassay_006780_11	euxassay_006780_12	euxassay_006780_13	euxassay_006780_14

euxassay_006780_15	euxassay_006780_16	euxassay_006780_17	euxassay_006780_18	euxassay_006780_19

euxassay_006780_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11561_wholemount_strong.wlz

11561_wholemount_moderate.wlz

11561_wholemount_weak.wlz

11561_wholemount_possible.wlz

11561_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11561_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

moderate

spotted

moderate expression: see section 08 09 15 16

adenohypophysis

strong

regional

strong expression: see section 10 11 12 13 14

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

pons mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 06 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 07 08 16 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 16 17

trigeminal v nerve

strong

regional

strong expression: see section 08 09 15 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 13 14 15

cervical ganglion

strong

regional

strong expression: see section 08 09 15 16

thoracic ganglion

strong

regional

strong expression: see section 10 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 15 16 17

neural retina

strong

regional

strong expression: see section 01 02 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 13 14 15 16 17

tongue

strong

spotted

strong expression: see section 12

tongue muscle

moderate

regional

moderate expression: see section 11 12

stomach

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07 08

midgut

moderate

spotted

moderate expression: see section 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7353
Entity Detected:	Sez6l2, seizure related 6 homolog like 2 ( MGI:2385295)
Sequence:	sense strand is shown >T7353 CGATGGGCCCAAGCTCCCGAACAAGGGGATAACCTTCGGCCCTCTCGGCCCCACCCCCCGCAGCGGGAGC CGCGCCCCCCTTTGCCATAGATCGCAGAGCAAAGGATAAAGATTGAGCAAAAGGGGGCGGACTCCAAGCA ACCTCTAGGCCAATTCTGCTTCTCCATCCCAAAGGAAGAAAATTTGCCTTCCTTTGCCGACCCTTAGCAG GAAAGCAAAATTAAAGGAAAAGATAAAAGGACGCAGAGGGAAAGTGACAGCGAGATTATTCAGAAGAGCC ACAGAGGAGAGAAATTAGTGTGAGTCGCCATGGGGACTCCCAAGGCCCAGCACCCGCCGCCTTCCCAGCT GCTGCTCCTAATTCTGCTGAGCTGTGCCTGGATTGAGGGTCTGCCCCTGAAGGAGGATGAGATGATGCCA GAGCCTGGAAGTGAGACTCCCACAGTGGCCTCTGAGGACCTGGCTGAGCTGCTCCATGGGGCTTTGCTGC GGAAGGGCCCAGAGATCGGCTTCTTGCCGGGATCTGACCCAGACCCCACACTAGCCACCCCTCCAGCCGG CCAGACTCTTGCAGCACCTTCCCTGCCACGTGCCACTGAGCCAGGGACAGGGCCTCTGACGACAGCTGTC ACCCCCAAGGGAGTCAGGGGGGCAGGCCCCACCGCGCCAGAGCTGCTGACCCCACCCCCTGGAACTACAG CCCCGCCCCCGCCTGGCCCCGCCTCCCCAGTCCCACCCCTCAGGCCTGAGGGAGGAGAGGAGGAAACCAC CACCACCATCATCACCACAACAACTGTCACCACCACTGTCACCAGCCCAGTTCTGTGCAATAACAACATC TCGGAGGGCGAAGGGTTTGTGGAGTCTCCGGACTTGGGGAGCACTGCCAGCCGCTCCGTGGAGCTCCTAG ATTGCACCTACAGCATCCATGTCTACCCTGGTTATGGCATTGAGATTCAGGTGCAGACACTCAACCTGTC TCAGGAGGAAGAACTTCTGGTGTTGGCCGGTGGGGGTTCTCCAGGCCTGGCCCCCAGACTCCTGGCCAAT TCCTCCATGCTGGGAGAAGGACAAGTGCTGCGGAGCCCAACAAACCGGCTGCTCCTGCATTTCCAGAGTC CTCGAGTCCCAAGGGGCAATGGCTTCAGGATTCACTATCAGGCATACCTTCTGAGCTGCGGCTTCCCACC CCGGCCAGCCCACGGGGATGTGAGCGTGACAGACTTGCATCCTGGGGGCACTGCCACCTTCCACTGTGAC TCAGGATACCAGCTACAGGGTGAGGAGACCCTCATTTGCCTCAATGGTACCCGGCCAGCCTGGACTGGTG AACCCCCTAGCTGTACAGCATCTTGTGGTGGCACCATCCACAATGCCACACTGGGTCGCATTGTATCCCC TGAGCCTGGGGGAGCTGCAGGGCCCAACCTCACCTGCCGCTGGGTCATTGAAGCGGCCGAGGGACGCCGG CTTCACCTACACTTTGAGAGAGTCTCACTGGATGAGGACAATGACCGGCTGATGGTGCGCTCAGGGGGAA GCCCCCTGTCCCCTGTGATTTACGACTCTGACATGGATGATGTCCCTGAACGCGGTCTCATCAGTGATGC CCAGTCCCTCTACGTAGAACTGCTTTCAGAGACACCAGCCAACCCCCTGCTGCTCAGCCTCCGATTTGAA GCCTTTGAGGAGGACCGCTGCTTCCCCCCGTTCCTGGCTCACGGCAATGTGACCACTACAGATCCTGAGT TCCACCCAGGAGCACTGGCCACCTTCTCATGCCTCCCAGGATATGCTCTGGAGCCACCAGGACCCCCCAA TGCTATCGAATGTGTGGATCCCACCGAACCCCACTGGAACGACACAGAGCCAGCCTGCAAGGCCATGTGT GGAGGAGAGCTGTCTGAGCCAGCTGGTGTGGTACTCTCTCCCGACTGGCCCCAGAGTTACAGCCCTGGCC AAGACTGCGTGTGGGGCCTGCACGTCCAGGAAGAGAAGCGCATCTTGCTCCAAGTTGAAATCTTGAATGT TCGCGAAGGGGATATGCTAACACTGTTCGACGGGGACGGTCCGAGCGCTCGAGTCCTGGCCCAGCTGCGA GGACCTCAGCCGCGCCGCCGCCTCCTCTCCTCTGGACCTGATCTCACTCTGCAGTTTCAGGCACCCCCTG GTCCCCCAAATCCGGGCCTAGGCCAGGGTTTCGTGTTACATTTCAAAGAGGTCCCGAGAAACGATACGTG CCCGGAGCTACCACCACCTGAGTGGGGCTGGAGGACAGCATCCCACGGGGACCTTATCCGGGGTACAGTG CTCACCTATCAATGCGAGCCTGGCTATGAACTACTGGGATCAGACATCCTCACTTGCCAATGGGACCTGT CCTGGAGCGCGGCGCCACCTGCCTGCCAAAAGATCATGACTTGTGCTGACCCTGGCGAGATCACCAATGG CCATCGGACTGCCTCAGATGCTGGTTTCCCTGTGGGCTCCCACGTCCAGTATCGCTGTCTGCCAGGGTAC AGCCTGGAAGGAGCAGCTGTGCTCACCTGCTACAGCCGGGACACAGGCACACCCAAGTGGAGTGATCGGG TCCCCAAATGCGCCTTAAAGTACGAGCCGTGCCTGAACCCCGGTGTTCCTGAGAATGGTTACCAAACTCT GTACAAGCATCACTACCAAGCGGGCGAGTCGCTACGCTTCTTCTGCTACGAGGGCTTTGAGCTCATCGGC GAGGTCACCATCACATGTGTACCCGGTCACCCCTCCCAGTGGACCAGCCAGCCCCCACTCTGCAAAGTGG CCTATGAGGAGCTCCTGGACAACCGAAAACTGGAAGTGACCCAGACTACAGACCCATCACGGCAGCTGGA GGGTGGTAATCTCGCTTTGGCCATCCTGTTGCCCCTGGGCTTGGTCATTGTCCTTGGCATTGGCGTTTAC ATATACTACACTAAGCTACAAGGAAAATCCCTCTTTGGCTTCTCGGGTTCTCACTCTTACAGTCCCATTA CGGTGGAGTCAGACTTCAGCAACCCACTGTATGAAGCTGGGGATACGAGGGAGTATGAAGTTTCCATCTG AGCCTCAAGTCTACAAGTCTGCAGGACCCAGGACCTCTGGTTCTCTCCTTGTTCTGGGCAGTGAGGAGCA TAGGACCTGGTCTCTGGCTCTCCTCCCCCCTGCTGTGTAAATAGTCTCCCCATCCCATGAGGGGGCTTTG ATGGCCCTGGAGACCCTACAGTAAATAAACCAGCATCGTGCCGCCCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191879 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191879 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11564 same embryo
	EMAGE:11563 same embryo
	EMAGE:11562 same embryo
	EMAGE:11560 same embryo
	EurExpress:euxassay_006780 same experiment
	MGI:4828008 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source