EMAGE mouse gene expression spatial database

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EMAGE:11564

Klhdc8a kelch domain containing 8A ( MGI:2442630)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006733. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006733_01	euxassay_006733_02	euxassay_006733_03	euxassay_006733_04

euxassay_006733_05	euxassay_006733_06	euxassay_006733_07	euxassay_006733_08	euxassay_006733_09

euxassay_006733_10	euxassay_006733_11	euxassay_006733_12	euxassay_006733_13	euxassay_006733_14

euxassay_006733_15	euxassay_006733_16	euxassay_006733_17	euxassay_006733_18	euxassay_006733_19

euxassay_006733_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11564_wholemount_strong.wlz

11564_wholemount_moderate.wlz

11564_wholemount_weak.wlz

11564_wholemount_possible.wlz

11564_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11564_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal cortex

strong

regional

strong expression: see section 06 07 08 12 13 14

hypothalamus mantle layer

strong

regional

strong expression: see section 08 09 10 11 moderate expression: see section 06 07 12 13 14

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 11 12 13 14

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 11 12 13 14 15 16 17 18

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 12 13 moderate expression: see section 05 07 08 09 10 11 14 15

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 11 12 13 14 15 16 17

pons mantle layer

strong

regional

strong expression: see section 06 07 08 09 12 moderate expression: see section 05 10 11 13 14 15

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 12 13 14 15 weak expression: see section 05 08

tegmentum

strong

regional

strong expression: see section 09 10 11 moderate expression: see section 07 08

facial vii ganglion

weak

regional

weak expression: see section 04 05 16

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 14 15

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 14 15 16 17 18

vagus x ganglion

moderate

regional

moderate expression: see section 06 13 14 15

trigeminal v nerve

weak

regional

weak expression: see section 06

spinal cord mantle layer

moderate

regional

moderate expression: see section 08 09 10 11

neural retina

moderate

regional

moderate expression: see section 01 02 03 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7347
Entity Detected:	Klhdc8a, kelch domain containing 8A ( MGI:2442630)
Sequence:	sense strand is shown >T7347 GGTTCTTTCCGCGGAGCCAGGCGTGGACGTCGCTGCCCCGCTGACACCCAAGGGACCCGGAGAAGCCAGC GGGCGTCGGCAGGGAGCCCGTGCAGCGCGAGATGGAGGCTGAGAGAGGGGACGCGCCGACCGGCGCCGTG TAGCCCGCGGGCCCCGGGGCTGGACCTTGCCCGGACGCTGTGGGGGCGCTCGAGCAGGGCCCTCCCCCGG GCTGCCATGGAAGTGCCCAATGTCAAGGACTTTCAGTGGAAGCGCCTTGCTCCACTGCCTAGCCGCCGGG TCTATTGCTCACTGCTGGAGACTGGGGGGCAGGTCTATGCCATCGGGGGATGTGATGACAATGGCGTCCC TATGGACTGCTTCGAGGTCTACTCCCCCGAGGCCGACCAGTGGACCTCCCTGCCCTCCCTGCCCACAGCC CGGGCCGGGGTAGCGATCACTGCCCTAGGAAAGCGGATTATGGTGATCGGGGGTGTGGGCACCAATCAAC TGCCTGTGAAGGTCGTGGAAATGTACAACATCGATGAGGGCAAGTGGAAGAAGAGAAGCGTCTTGCGCGA GGCTGCCATGGGCATTTCTGTCACAGCCAAAGATTATCGAGTGTATGCCGCAGGAGGGATGGGCCTGGAC CTACGTCCACACAACTACTTGCAACACTATGACATGCTGAAGGACATGTGGGTGTCACTAGCTCCCATGC CCACCCCAAGATACGCTGCCACCTCCTTCCTCCGGGGCTCCAAGATCTATGTGCTGGGGGGACGACAATC TAAGTATGCAGTCAATGCCTTCGAGGTCTTTGATATAGAGTCTCGGTCCTGGACCAAGTTCCCCAACATT CCCTGTAAACGGGCTTTCTCCAGCTTTGTGACCCTGGACAACCACTTATACAGCCTAGGAGGCCTGCGCC AGGGCCGTCTGTACCGGCAGCCCAAGTTCCTCCGGACAATGGATGTGTTCGACATGGAACAGGGGGGATG GCTGAAGATGGAACGCTCATTCTTCCTCAAGAAACGCCGGGCAGACTTTGTGGCTGGTGGTCTGAGTGGA AGGGTCATAGTGGCTGGGGGCCTTGGAAACCAGCCCACTGTCCTGGAGACAGCAGAAGCATTCCACCCTG AGAAGAACAAGTGGGAGGCACTCCCCCCCATGCCCACGCCCCGCTGTGCCTGCTCCAGCATTGTCTTCAA GAACTGCCTCCTGGCTGTGGGGGGTGTCAGCCAGGGACTGAGCGATGCGGTAGAGGCACTGTTTGTCTCT GACTCCTAGCTGGCCGGAGCAGCACCTTTGCCTGGTCAGCATCACTCCAATCCTGACATGAGGAATGGTC TCATCAAAGCAGTTTGGACTACTTCCTCAGGCTCCTACTCCTGTGAAGAGACAGAGAGGTCAAGCCCTGG GGCTCTCAGTGACCTCTCTCTAACCCTGCACTCTCAGAAAGCAGAGCTGACTCCGCCAGTCAGTCAGTGC TGGGCTGTGGACTGGGGAGAAGCTTCACTCAGTGCTCAGACACAGCAGGGCAAAGTTCCCTCCTCTCGTC TACCTCGTGAGGTGGAGGGCATTGCCACCCAGAGGGAGATCCTTGTTGTCCTTCCCCATCTCTGAGGTAG TTTGAGTGTCGTGAGAGATAAACTAATGAAGAACAGGGCACCCCCAATCCCTTGTTTCTCTTTGGTTGAC ATCCATTCCCCATATAGAAGTCAACTGTAGACCTGCCCCAGATCTCACCTACCTATCAGGAATTGCACTT GAAGCTAGGGCAGTTGTGTAGGAAGGAAGCTGGGGGCTCACACACCAGGCCAACAGCTTTGGCAGAAGAG ACTCTTCCTCTCAGGTCCCAGTTGGGCATGTGATGATGGAGTGTCAATATCTTCTGTATTCTGCCTTCTG CCACCTTCTCCTGACCTGAGGCCTGGCCACTCTGTGTCCCAACAGCTCTCAGAGTGTTTCTGCGTGAAAC TGTCTCATTTACATACACTGTGGCTTCCAAATCCACAAAGGGTGGATTAATTGCACTCCGGTTAATAACA GCAGCACAACAATTGGCATCTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191503 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191503 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11563 same embryo
	EMAGE:11562 same embryo
	EMAGE:11560 same embryo
	EMAGE:11561 same embryo
	EurExpress:euxassay_006733 same experiment
	MGI:4825791 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source