EMAGE mouse gene expression spatial database

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EMAGE:11566

Mst1 macrophage stimulating 1 (hepatocyte growth factor-like) ( MGI:96080)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006752. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006752_01	euxassay_006752_02	euxassay_006752_03	euxassay_006752_04

euxassay_006752_05	euxassay_006752_06	euxassay_006752_07	euxassay_006752_08	euxassay_006752_09

euxassay_006752_10	euxassay_006752_11	euxassay_006752_12	euxassay_006752_13	euxassay_006752_14

euxassay_006752_15	euxassay_006752_16	euxassay_006752_17	euxassay_006752_18	euxassay_006752_19

euxassay_006752_20	euxassay_006752_21	euxassay_006752_22	euxassay_006752_23	euxassay_006752_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11566_wholemount_strong.wlz

11566_wholemount_moderate.wlz

11566_wholemount_weak.wlz

11566_wholemount_possible.wlz

11566_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11566_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

esophagus epithelium

weak

regional

weak expression: see section 14 15

pharynx epithelium

weak

regional

weak expression: see section 13 14 15 16

stomach proventricular region epithelium

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7421
Entity Detected:	Mst1, macrophage stimulating 1 (hepatocyte growth factor-like) ( MGI:96080)
Sequence:	sense strand is shown >T7421 GTCACCGTGCAGCCTCCAGCTTAGGAGAATGGGGTGGCTCCCACTTCTGCTGCTTCTGGTACAGTGTTCA AGGGCTCTTGGGCAGCGCTCACCACTGAATGACTTCCAGCTGTTCCGGGGCACAGAGTTAAGGAACCTGT TACACACAGCGGTGCCGGGGCCATGGCAGGAGGATGTGGCAGATGCTGAGGAGTGTGCTAGGCGCTGTGG GCCCCTTCTGGACTGTCGGGCCTTCCACTACAACATGAGCAGCCATGGTTGCCAGCTGCTGCCGTGGACC CAGCACTCGCTGCACACACAGCTATACCACTCGAGTCTGTGCCATCTCTTCCAGAAGAAAGATTATGTGC GGACCTGCATTATGGACAATGGGGTCAGCTACCGGGGCACTGTGGCCAGGACAGCTGGTGGCCTGCCCTG CCAAGCCTGGAGTCGCAGGTTCCCCAATGACCACAAGTATACGCCCACGCCAAAGAATGGCCTGGAAGAG AACTTCTGTAGGAACCCTGATGGGGATCCCAGAGGTCCCTGGTGCTACACAACAAACCGCAGTGTGCGTT TCCAGAGCTGTGGCATCAAAACCTGCAGGGAGGCTGTTTGTGTTCTGTGCAACGGTGAGGATTACCGTGG CGAGGTAGACGTTACAGAGTCAGGGCGGGAGTGTCAACGCTGGGACCTGCAGCACCCCCACTCGCACCCT TTCCAGCCTGAAAAGTTCCTAGACAAAGATCTGAAAGACAACTATTGTCGTAATCCGGACGGATCTGAGC GGCCCTGGTGCTACACCACAGACCCGAATGTTGAGCGAGAATTCTGCGACCTGCCCAGTTGCGGGCCTAA CCTGCCTCCGACCGTCAAAGGATCCAAGTCACAGCGGCGCAACAAGGGCAAGGCTCTTAACTGCTTCCGC GGAAAAGGTGAAGACTATCGAGGCACAACCAATACCACCTCTGCGGGCGTGCCCTGCCAGCGGTGGGATG CGCAGAGTCCACACCAGCACCGCTTTGTGCCAGAGAAATATGCTTGCAAGGACCTTCGTGAGAATTTCTG CCGGAATCCTGATGGCTCCGAGGCGCCTTGGTGCTTCACATCTCGACCTGGTTTGCGCATGGCCTTCTGC CACCAGATCCCACGCTGCACTGAAGAACTGGTGCCAGAGGGATGCTACCACGGCTCAGGTGAACAGTATC GTGGCTCAGTCAGCAAGACGCGCAAGGGCGTTCAGTGCCAGCACTGGTCCTCTGAGACACCGCACAAGCC ACAATTTACACCCACCTCGGCACCGCAGGCGGGACTGGAGGCCAACTTCTGCAGGAATCCTGATGGGGAT AGCCATGGGCCCTGGTGCTATACCTTGGACCCGGATATCCTGTTTGACTACTGTGCCCTACAGCGCTGTG ATGATGACCAGCCACCATCCATTCTGGACCCCCCAGACCAGGTGGTGTTTGAAAAGTGTGGCAAGAGAGT TGACAAGAGTAATAAACTTCGTGTGGTGGGAGGCCATCCTGGGAACTCCCCATGGACGGTCAGCTTGCGG AATCGACAGGGCCAGCATTTCTGTGGGGGCTCCCTAGTGAAGGAGCAGTGGGTACTGACTGCCCGGCAAT GCATCTGGTCATGCCACGAACCTCTCACAGGATACGAGGTATGGTTGGGTACAATTAACCAGAACCCACA GCCTGGAGAGGCAAACCTGCAGAGGGTCCCAGTGGCCAAGGCAGTGTGCGGCCCTGCAGGCTCCCAGCTT GTTCTGCTCAAGCTGGAGAGACCTGTGATCCTGAACCATCACGTGGCCCTGATTTGCCTGCCTCCTGAAC AGTATGTGGTACCTCCAGGGACCAAGTGTGAGATCGCAGGCTGGGGTGAATCCATCGGTACAAGCAATAA CACAGTCCTTCATGTGGCCTCGATGAATGTCATCTCCAACCAGGAATGTAACACGAAGTACCGAGGACAC ATACAAGAGAGTGAGATATGCCCCCAGGGAATGGTGGTCCCTGTGGGGGCTTGTGAGGGTGACTACGGGG GCCCACTTGCCTGCTATACCCATGACTGCTGGGTCCTACAGGGACTTATCATCCCGAACAGAGTGTGTGC ACGGCCCCGCTGGCCAGCTATTTTCACACGGGTGTTTGTGTTTGTGGACTGGATTAACAAGGTCATGCAG CTGGAGTAGGCCTGCTTTTGAGCCCTTAGAGATGTCAAGACTTTTCAAACATAAAGCGGCCTTTTTTTTT TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4195560 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4195560 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11570 same embryo
	EMAGE:11568 same embryo
	EMAGE:11569 same embryo
	EMAGE:11567 same embryo
	EMAGE:11565 same embryo
	EurExpress:euxassay_006752 same experiment
	MGI:4826472 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source