EMAGE mouse gene expression spatial database

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EMAGE:11584

Adar adenosine deaminase, RNA-specific ( MGI:1889575)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006621. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006621_01	euxassay_006621_02	euxassay_006621_03	euxassay_006621_04

euxassay_006621_05	euxassay_006621_06	euxassay_006621_07	euxassay_006621_08	euxassay_006621_09

euxassay_006621_10	euxassay_006621_11	euxassay_006621_12	euxassay_006621_13	euxassay_006621_14

euxassay_006621_15	euxassay_006621_16	euxassay_006621_17	euxassay_006621_18	euxassay_006621_19

euxassay_006621_20	euxassay_006621_21	euxassay_006621_22	euxassay_006621_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11584_wholemount_strong.wlz

11584_wholemount_moderate.wlz

11584_wholemount_weak.wlz

11584_wholemount_possible.wlz

11584_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11584_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 weak expression: see section 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 weak expression: see section 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 09 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 19

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7073
Entity Detected:	Adar, adenosine deaminase, RNA-specific ( MGI:1889575)
Sequence:	sense strand is shown >T7073 GCCACAGGTGCGGGCCTTGCCGGCACTATGTCTCAAGGGTTCAGGGGACCCACAGGGGTGTTCCCTCACC AGACACAGTCGTACTCGGACCCTAGTCATGAGCATAGCAAGTGGAGATACCTGCAGCCACAGGGGCCGGA GTCTTACCCTAGGAGTTTCCAGCTTCAGCAGATAGAGTTTCTCAAAGGGCGGCTCCCAGAAGCTCCCTTG ATTGGAATACAAACCCAGTCACTGCCGCCATTCCTCCCAGGACACTGGCCAAGATTCCCAGGGCCACCTG CCCAAGACAGGCAACTGGAAATCTGGGAGTTCCCCAGGAGTGTGACTCTCAGAAATCAGGGGTTCCACAT AGGACCCCCACTTCCTCCCCCACACAGCAGGGGTCCACCATGGAGAGGTGCTGACGGGCTTTGCTCACAC TTCCGGGAGCTGAGCATCAGTCAGAGTCCGGAGCAGAAGGTTCTAAACCGCCTGGAAGAGCTTGGGGAGG GGAAGGCCACCACTGCCCATGTGCTAGCCAGAGAGCTCAGAATCCCCAAAAGGGACATCAATCGTATTTT GTACTCCCTGGAAAAGAAGGGAAAGCTGCACAGAGGAAGGGGGAAACCTCCTTTGTGGAGCCTTGTGCCC TTGAGTCAGGCTTGGACTCAGCCCCCTGGAGTTGTGAATCCAGATAGTTGTATCCAGGAATTCCCTAGAG GAGAGCCTGGTTTGGACAGTGAGGACGGAGACCCTGCCTCTGACTTAGAAGGACCTTCTGAGCCTCTTGA CATGGCTGAAATCAAGGAGAAGATCTGTGACTATCTGTTCAATGTGTCAAACTCCTCTGCCCTGAACCTG GCTAAGAACATTGGCCTCACCAAGGCCCGAGATGTGACCTCAGTGCTGATTGACTTGGAAAGGCAAGGCG ATGTCTACAGGCAAGGGGCAACTCCTCCCATCTGGTACTTGACGGACAAGAAGCGTGAGAGGCTGCAGAT GAAGAGAAGTACACACAGTGCTCCTGCCCCTACCCTGACAGCTGTCCCAGAGGCCACTAGAAGCCCCTCA TTCCCTGCCTGCCACCCGCCCCCAGCAGGTGCCTCAAGCAGTGTGGCAGCCTCCAAGAGAGTGGAGAATG GGCAGGAGCCTGCGATAAAGCATGAAAGTAGGCATGAGGCCAGACCAGGACCAATGAGACTGCGGCCTCA CGCTTATCACAATGGCCCCTCTAGAGCAGGGTATGTGGCCTCTGAAAATGGCCAGTGGGCCACAGATGAC ATCCCAGATAACTTGAATAGTATCCACACAGCACCAGGTGAGTTTCGAGCCATCATGGAGATGCCCTCCT TCTACAGCCCTACCTTGCCACGGTGTTCACCCTACAAGAAGCTAACTGAGTGCCAGCTGAAGAACCCTGT CAGCGGGTTGTTAGAGTATGCTCAGTTCACTAGTCAGACCTGTGATTTCAACCTGATAGAGCAGAGTGGA CCGTCCCATGAACCTCGATTTAAATTCCAGGTTGTCATCAATGGGCGGGAATTTCCCCCAGCTGAGGCTG GCAGCAAGAAAGTAGCCAAGCAGGACGCAGCAGTGAAAGCCATGGCGATTCTGCTTCGGGAAGCCAAAGC CAAAGACAGTGGTCAACCAGAAGACTTGTCCCACTGTCCCATGGAAGAAGACTCGGAGAAACCAGCAGAG GCTCAGGCCCCCAGCTCCTCAGCAACATCCTTGTTCTCTGGGAAGAGCCCAGTTACTACACTACTTGAGT GCATGCACAAACTAGGGAACTCCTGTGAATTCCGTCTCCTGTCCAAAGAAGGCCCTGCTCATGACCCCAA GTTCCAGTACTGTGTAGCAGTAGGAGCCCAGACCTTCCCCCCTGTGAGCGCCCCCAGCAAGAAGGTAGCA AAGCAGATGGCCGCAGAGGAAGCCATGAAGGCGCTGCAGGAGGAGGCAGCCAGTTCAGCGGATGACCAGT CTGGAGGTGCGAACACAGACTCACTTGATGAATCTATGGCTCCCAACAAGATCAGGAGGATTGGTGAGCT CGTCAGGTACCTGAACACCAACCCCGTAGGCGGCTTGTTGGAGTACGCCCGATCTCATGGCTTTGCTGCT GAGTTCAAGCTCATTGACCAGTCTGGACCTCCTCACGAACCCAAGTTTGTTTACCAAGCAAAAGTTGGGG GCCGCTGGTTTCCAGCCGTGTGTGCACACAGCAAGAAACAGGGCAAGCAGGATGCAGCGGATGCAGCCCT CCGGGTCTTGATCGGGGAGAGCGAGAAGGCAGAGCAGTTGGGTTTCGCAGAGCTTCCTCTCTCTGGCAGC ACCTTCCACGACCAGATAGCTATGCTGAGCCACAGGTGCTTCAATGCTCTGACCAACAGTTTCCAGCCCT CCCTGCTCGGCCGCAAGATCCTGGCTGCCATTATTATGAAAAGAGATCCAGAGGACATGGGTGTTGTCGT GAGTTTGGGGACAGGCAATCGCTGTGTGAAAGGGGACTCTCTCAGCCTGAAGGGAGAGACGGTCAATGAC TGCCATGCCGAAATCATCTCCCGGAGGGGCTTCATCAGGTTTCTCTACAGTGAACTGATGAAGTACAACC ACCACACTGCCAAGAACAGCATATTTGAGCTTGCCAGGGGAGGAGAGAAGCTGCAGATAAAGAAGACGGT TTCTTTTCATCTCTACATCAGCACGGCGCCATGTGGAGATGGAGCCCTCTTTGACAAATCCTGCAGTGAC CGTGCCGTGGAAAGCACAGAGTCCCGCCATTACCCTGTCTTTGAAAATCCCAAGCAAGGCAAGCTTCGCA CCAAGGTGGAGAATGGGGAAGGCACAATTCCTGTGGAGTCCAGTGATATTGTACCCACGTGGGATGGCAT CCGCCTTGGGGAAAGACTCCGTACCATGTCCTGTAGTGACAAAATCCTACGCTGGAATGTGCTGGGCCTG CAAGGGGCGTTGTTGACGCACTTCCTACAGCCTGTGTACCTGAAATCTGTAACCTTAGGTTACCTTTTCA GCCAAGGGCATCTGACCCGTGCTATTTGCTGCCGCGTGACCAGAGATGGGAAAGCATTTGAGGATGGACT ACGCTATCCCTTTATTGTCAACCACCCCAAGGTCGGCCGAGTCAGTGTTTATGATTCCAAAAGACAGTCC GGAAAGACCAAGGAAACAAGCGTCAACTGGTGCATGGCTGATGGCTATGACCTAGAGATCCTGGATGGCA CCAGAGGCACTGTGGATGGACCAGGGAAAGAGTTGTCTCGGGTGTCCAAGAAGAATATTTTCCTTCAGTT TAAGAAGCTCTGCTCCTTCCGAGCCCGCAGAGATTTACTGCAGCTCTCTTATGGTGAAGCCAAGAAAGCT GCCCGTGACTATGACTTAGCCAAGAACTACTTCAAGAAAAGCCTGCGAGACATGGGCTATGGGAATTGGA TCAGCAAACCCCAGGAGGAAAAGAACTTTTACCTCTGTCCAGTACCCAATGACTGATAGTGGGGCGCGTT TCTCCTGGGGTCAGAGGGCGGTCATGGCATTCCTCATCACACCGGGCCAGAGGATAGGAGCTTTTTTTAC CCACTCCCCCCTTTTTTAATGGTAGAACCATAATAGATGGTACCAAGAACTGCTTTCTTTGGGATTTCGA GGTGGGGTCCAGCCAAGTCCCCACCTCCCTTTTCTCAAGGGAAGAGGCCAAGATTAAAGGAAATGGAAAT GCTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4012994 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4012994 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11583 same embryo
	EMAGE:11585 same embryo
	EurExpress:euxassay_006621 same experiment
	MGI:4822994 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source