EMAGE mouse gene expression spatial database

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EMAGE:11590

Isy1 ISY1 splicing factor homolog (S. cerevisiae) ( MGI:1923310)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006618. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006618_01	euxassay_006618_02	euxassay_006618_03	euxassay_006618_04

euxassay_006618_05	euxassay_006618_06	euxassay_006618_07	euxassay_006618_08	euxassay_006618_09

euxassay_006618_10	euxassay_006618_11	euxassay_006618_12	euxassay_006618_13	euxassay_006618_14

euxassay_006618_15	euxassay_006618_16	euxassay_006618_17	euxassay_006618_18	euxassay_006618_19

euxassay_006618_20	euxassay_006618_21	euxassay_006618_22	euxassay_006618_23	euxassay_006618_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11590_wholemount_strong.wlz

11590_wholemount_moderate.wlz

11590_wholemount_weak.wlz

11590_wholemount_possible.wlz

11590_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11590_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7053
Entity Detected:	Isy1, ISY1 splicing factor homolog (S. cerevisiae) ( MGI:1923310)
Sequence:	sense strand is shown >T7053 CCACGCGTCCGAAAGACCAGACTGTCCGCTGTTTTCCGTTCCCGCGTCTGCCTGCCTTGTTCCGGGCGCC TGAGCACGTTGCGTTGCAGAGCGGCGCCAGGGCGCCCTCGCCGGCACCATGGCCCGAAATGCAGAAAAAG CCATGACTGCCCTGGCGAGGTTTCGTCAAGCTCAGCTGGAAGAGGGAAAAGTCAAGGAACGGAGACCTTT CCTTGCTTCCGAATGTACTGAGTTGCCGAAAGCGGAGAAGTGGAGACGGCAGATCATTGGCGAGATCTCC AAAAAGGTTGCTCAGATTCAGAATGCTGGCTTAGGGGAATTCCGAATTCGGGACCTGAATGACGAAATTA ACAAGCTGCTGAGGGAGAAAGGACACTGGGAGGTCCGCATCAAGGAGCTGGGAGGTCCTGATTATGGAAA AGTTGGCCCCAAGATGCTGGATCATGAAGGTAAAGAAGTCCCAGGAAATCGAGGTTACAAGTACTTTGGA GCAGCAAAAGATTTGCCTGGTGTCAGAGAGCTATTTGAGAAAGAACCCCTTCCTCCTCCCAGAAAGACGC GTGCTGAGCTCATGAAGGCCATCGATTTTGAATACTATGGTTACCTGGATGAAGATGATGGAGTTATTGT GCCCTTGGAACAAGAGTATGAGAAGAAACTCAGAGCTGAGCTGGTGGAAAAATGGAAGGCAGAGAGAGAA GCTCGGCTGGCAAGGGGAGAAAAGGAAGAGGAGGAGGAGGAGGAAGAGGAGATCAACATCTATGCTGTCA CGGAGGAGGAGTCTGATGAGGAAGGCAACCAGGAGAAGGCTGGGGAAGATGGTCAGCAGAAGTTCATTGC TCATGTGCCGGTGCCATCTCAGCAGGAGATTGAGGAGGCGCTTGTACGGAGGAAGAAAATGGAACTGCTC CAGAAATATGCAAGTGAGACCCTGCAGGCACAGAGTGAAGAAGCCAAGCGACTCCTGGGGTACTAGGCTG AGCCAGAGCCCTTCTTGAACTCTGGAAGACCTGGTGGGTGTGCTTGGTACGCCATGGCACGCCATCCTCA TAGCTGCCCTGGGCTGTGGCTAGGCTGCCTGGCGCTGGAAGGCCTGTGCCTTTCTCACCCTTCAGCTTAC CTCCATTTCTGGAATGTGAGCTGGCCTGATAGTAGGGCCCTGTCCCTTCCCAGGAAGCTGCCAGGTACTG AATGCCTTAGTAGCCATCGCCTTTGTACAGATGCCTTTGCTACCGTGTGCATCCATTCATTTTTGTCTTG GAATGTGTAAAATCTGTTCTCTGAAAATTTACTTTGCCCTATACTCTGCTTTTGGGAATCAAGAAGGATC AAACTAGTAGCCAATTTTCAAAAATACATAATTTTTATTCAAATCACATCTAAATTTAGAGACACATCTT CTCTGGGAGACTGAGATGGTGTATCGACCCTGAGATGGAATTCCGTGGTCAGTGCTGGAGCCATCCCTGC GTCTGGGCACTGCTTACCTTGCCATGATCCCCCTCTGGGAGAGCCATTTCACCTTCTCCTGTCCTGTGGG CAGGAGTTTTGAGTTAGCGACTTTATTTTTATAACTTGTTTCAGAGAAAGGTACTTTTATTAAAGGTTTT TAATAAGAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4009615 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4009615 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11587 same embryo
	EMAGE:11589 same embryo
	EMAGE:11588 same embryo
	EMAGE:11586 same embryo
	EurExpress:euxassay_006618 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source