EMAGE mouse gene expression spatial database

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EMAGE:11602

Ube2d3 ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) ( MGI:1913355)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006830. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006830_01	euxassay_006830_02	euxassay_006830_03	euxassay_006830_04

euxassay_006830_05	euxassay_006830_06	euxassay_006830_07	euxassay_006830_08	euxassay_006830_09

euxassay_006830_10	euxassay_006830_11	euxassay_006830_12	euxassay_006830_13	euxassay_006830_14

euxassay_006830_15	euxassay_006830_16	euxassay_006830_17	euxassay_006830_18	euxassay_006830_19

euxassay_006830_20	euxassay_006830_21	euxassay_006830_22	euxassay_006830_23	euxassay_006830_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11602_wholemount_strong.wlz

11602_wholemount_moderate.wlz

11602_wholemount_weak.wlz

11602_wholemount_possible.wlz

11602_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11602_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8197
Entity Detected:	Ube2d3, ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) ( MGI:1913355)
Sequence:	sense strand is shown >T8197 GGCACGAGGGCGGACATCTTGTGGCTGTGCCGTGCGCGTGAGCCCCGTAGGGCCGGGGAGGCACCAGCTG CCGCGCGGGGAGGAGGCCGAGGCCGCAGCTCGAGGGAGGCCCCGGCCCTCTGCGCCTGTGTCTGGCAGAG CCGGTGTGAGAAGAAGAGACAACCCTTCCCCGCCGCCGGGATAATCAAGAGTTTTGGCCGGACCTTTGAG TACACCTCGGGATAGTGAGGAGCCCGGCGAAAAGCACAGACTATGGCGCTGAAACGGATTAATAAGGAAC TTAGTGATTTGGCCCGTGACCCTCCAGCACAATGTTCTGCAGGTCCAGTTGGAGATGACATGTTTCATTG GCAAGCCACAATTATGGGACCTAATGACAGCCCATATCAAGGTGGTGTATTCTTTTTGACAATTCATTTT CCTACAGACTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACA GTAATGGCAGCATTTGTCTTGATATTCTAAGATCACAGTGGTCTCCTGCTTTAACTATTTCTAAAGTTCT TTTATCCATTTGTTCACTGCTATGTGATCCAAACCCAGACGACCCCCTAGTGCCAGAGATTGCACGGATC TATAAAACAGACAGAGATAAGTACAACAGAATATCTCGGGAATGGACTCAGAAGTATGCCATGTGATGCT ACCTTACAGTCAGAATAACCTGCATTATAGCTGGAATAAACTTTAAATTACTGTTCCTTTTTTGATTTTC TTATCCGGCTGCTCCCCTATCAGACCTCATCTTTCTTAATTTTATTTTTTGTTTACCTCCCTCCATTCAT TCACATGCTCATCTGAGAAGACTTAAGTTCTTCCAGCTTTGGACAATAACTGCTTTTAGAAACTGTAAAG TAGTTACACGAGAACAGTTGCCCAAGATTCAGAAATTTTTTTAAAAACTGGAGCATGTGTATTATATGGC CAATGTCTTCACTCTAACTTGGTTATGAGACTAAACCAATCCTCACTGCTCTAACATGCTGAAGAAGCCA TCTGAGGGGGAGGGAGATGGATGCTCAGCTGTCACATCAAAGGAAGCAGCATTATTCTAGCAGCATCCGT TCTTGTTTAAACCTTCCACTGTTAGAGGTTTGAGGTTACATGATATACTTTATGCTCATAACTGATGTGG CTGGAGAATTGGTATTGAATTATAGCATCAGCAGAACAGAAAATGTGATGTATTTTATGCATGTCAATAA AGGAATGACCTGTTCTTGTTCTACAGAGAATGGAAATTGGAAGTCAAACACCCTTTGTATTCCAAAATAG GGTCTCAAAACATTTTGTAATTTTCATTTAAATTGTTAGGAGGCTTGGAGCTATTAGTTAATCTATCTTC CAATACTGTTTAATATAGCACTGAATAAATGATGCGAGTTGTCAATGGATGAGTGATCAACTAATAGCTC TCCTAGTAATTGATTTATTTTTCTTCAATAAAGTTGCATAAACCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:963757 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:963757 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11605 same embryo
	EMAGE:11607 same embryo
	EMAGE:11603 same embryo
	EMAGE:11604 same embryo
	EMAGE:11606 same embryo
	EurExpress:euxassay_006830 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source