EMAGE mouse gene expression spatial database

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EMAGE:11608

Gucy1b3 guanylate cyclase 1, soluble, beta 3 ( MGI:1860604)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006987. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006987_01	euxassay_006987_02	euxassay_006987_03	euxassay_006987_04

euxassay_006987_05	euxassay_006987_06	euxassay_006987_07	euxassay_006987_08	euxassay_006987_09

euxassay_006987_10	euxassay_006987_11	euxassay_006987_12	euxassay_006987_13	euxassay_006987_14

euxassay_006987_15	euxassay_006987_16	euxassay_006987_17	euxassay_006987_18	euxassay_006987_19

euxassay_006987_20	euxassay_006987_21	euxassay_006987_22	euxassay_006987_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11608_wholemount_strong.wlz

11608_wholemount_moderate.wlz

11608_wholemount_weak.wlz

11608_wholemount_possible.wlz

11608_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11608_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

weak

regional

weak expression: see section 09 10 11 19 20 21

thymus primordium

moderate

regional

moderate expression: see section 13 16 weak expression: see section 14 15 17

pancreas

weak

regional

weak expression: see section 10 11 12

pituitary gland

weak

regional

weak expression: see section 12 13 14 15 16

vibrissa

weak

regional

weak expression: see section 06 07 22 23

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 13 14 15 16 17

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 18 weak expression: see section 02 03 04 05 06 07 08 19 20 21 22

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 10 11 12 15 16 17 18

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 12 13 14 15 16 17 18

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 19 21 moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 20

pons mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 14 15 16 17 18 19 20

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

dorsal grey horn

moderate

regional

moderate expression: see section 13 14

ventral grey horn

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17

eye skeletal muscle

moderate

regional

moderate expression: see section 05 06 07 21 22

lens

strong

regional

strong expression: see section 01

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 17 18

aorta

moderate

regional

moderate expression: see section 13 14 15 16 17

heart ventricle

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

esophagus

strong

regional

strong expression: see section 14

tongue

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 11 12 16 17 18

stomach

strong

regional

strong expression: see section 09 10 moderate expression: see section 02 03 04 05 06 07 08 11 12

hindgut

strong

regional

strong expression: see section 13 14 15 16

rectum

strong

regional

strong expression: see section 15 16

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 08

lower lip

weak

regional

weak expression: see section 07 08 22

upper lip

weak

regional

weak expression: see section 05 06 07 08 22

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

bladder

weak

regional

weak expression: see section 14 15 16 17

metanephros

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20 21

testis

weak

regional

weak expression: see section 06 07 08 09 21 22

urethra of male

moderate

regional

moderate expression: see section 15 16 17

lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9112
Entity Detected:	Gucy1b3, guanylate cyclase 1, soluble, beta 3 ( MGI:1860604)
Sequence:	sense strand is shown >T9112 CTAGCTTCAGCTCTGTGCTGGTCTGGGAGCACTGTCGCCGCTGTAGTCGCTGTCTGGGTCCCTTCCGCGG TACTCTTGCCTGGAAGCTGCTTCCTAGGCTCCGGGCGCGGACACCATGTACGGTTTCGTGAACCATGCCC TGGAGCTGCTGGTGATCCGCAATTATGGTCCCGAGGTGTGGGAAGACATCAAAAAAGAGGCACAGCTGGA TGAAGAAGGCCAGTTTCTTGTCAGAATAATATACGATGATTCCAAAACATATGACCTGGTGGCTGCTGCA AGCAAAGTCCTCAACCTCAATGCTGGCGAAATCCTGCAGATGTTTGGGAAGATGTTTTTCGTCTTCTGCC AGGAGTCTGGCTATGATACCATCTTGCGTGTCCTGGGATCTAATGTCAGAGAGTTTTTGCAGAACCTCGA TGCCCTGCATGACCACCTCGCCACCATTTACCCAGGGATGCGCGCGCCTTCCTTCAGGTGCACCGATGCG GAGAAAGGCAAAGGGCTCATCCTGCACTACTACTCGGAAAGAGAGGGGCTTCAGGACATCGTGATCGGGA TTATCAAGACTGTTGCTCAACAGATACACGGCACTGAGATAGACATGAAGGTGATTCAGCAAAGAAATGA AGAATGTGATCACACCCAGTTTTTAATTGAAGAAAAGGAATCAAAAGAAGAGGATTTTTATGAAGATCTG GATAGGTTTGAAGAGAATGGTACCCAGGAATCACGTATCAGCCCTTACACCTTCTGCAAAGCATTTCCTT TTCACATCATATTTGACCGGAACCTAGTGGTCACTCAGTGTGGCAATGCCATCTACAGAGTGCTCCCCCA GCTCCAGCCTGGGAACTGCAGCCTTCTGTCTGTCTTCTCTCTGGTCCGCCCTCACATCGACATCAGTTTC CATGGGATTCTTTCACACATCAATACAGTCTTTGTACTGAGAAGCAAGGAAGGGTTGCTGGATGTGGAGA AACTTGAGTGTGAGGATGAACTGACTGGAGCAGAGATTAGCTGCTTACGTCTCAAAGGCCAAATGATCTA CTTACCAGAAGCAGATAGCATCCTCTTCCTCTGTTCACCAAGTGTGATGAACCTGGACGACCTAACAAGA AGAGGCCTGTATCTGAGTGACATCCCTCTCCACGATGCTACCCGAGACCTGGTTCTTTTGGGAGAACAGT TCCGGGAGGAGTACAAACTGACACAAGAGCTGGAAATCCTCACCGACAGGCTGCAGCTCACACTGAGAGC CTTGGAGGATGAGAAGAAAAAGACAGACACATTGCTGTATTCTGTCCTTCCTCCATCTGTTGCCAATGAG CTGAGACACAAGCGCCCAGTGCCTGCCAAAAGATACGACAATGTGACCATCCTCTTCAGCGGCATTGTGG GCTTCAATGCTTTCTGTAGCAAGCATGCATCTGGAGAAGGGGCCATGAAGATTGTCAATCTCCTCAACGA TCTCTACACCCGATTTGACACACTGACTGATTCACGAAAAAACCCATTTGTTTACAAGGTGGAAACAGTT GGTGACAAGTATATGACAGTGAGTGGCTTGCCAGAACCTTGTATCCACCATGCACGGTCCATTTGCCACC TGGCTTTAGACATGATGGAAATTGCTGGTCAAGTTCAAGTAGATGGTGAATCTGTTCAGATAACAATCGG GATCCATACCGGGGAGGTGGTGACAGGTGTGATTGGACAGCGGATGCCTCGGTATTGTCTCTTTGGGAAT ACCGTCAACCTCACAAGCAGGACAGAAACCACAGGAGAAAAGGGAAAGATAAATGTTTCCGAATATACAT ACAGAAAACTCGGATCCACTGTTCCATTTGGAGCACAGAGGCCCAGTGTCTATGAAGGGCAAGAAGGAAC CAATGCAAGTCTGGTTCCTATCCAGGAAAAATACAGGCACGGAGGAAACAAATGAGGAGGATGAAAACTG AGTGTCAGGTTGTGGAACAAAGAGAACGTCGGTTGGGTTCGGGTGACAGTCTAATGTGTGTCAAGCAAGG AGCACTTCTTCCCTGTGGATAGCAATTTCTACTTCGTGTTTTAGTGGCCCAAGGCTTTCTCCTAGTTACA CAGATCTCACACTATGGTTTATTTGATTTTAGCTCTGCTTTCGATTACTTTTAAGGTCTCAGTATATTTT CCAAAGTTTGGGTTTTTGATGTGGATACTTGAGCTGTTTCTTAAATTCTGCTACAAGCATTGCCTAGACG GACGTTCTAAAAGTGATAAGCACCCACTGTGTTAAGTTTGTTAAATCTGATAGAACGAGACTTAATAGTA TCTGGCCATGCGTGTATATATCATGGCTCAGTAGATTTGTTTTATGCTCCATGTATATGTGTGTGTATAT GTATATTTTAATGACTATACCATAAAACAAAGTTTATATCATGTTGGTGCATGGCATTCTAGAAACCATT TTGTACACGAGTGAATCTAAGTTTTAGGGAAAAAAGGCAATTTATTTGTAGACTTCTGAAGTAAGAATTA GTATGCTATATTAGGAAAAGGAGTGACTATTTTGAAGTATGTCAATTCCCTTCTGGGACTCTATTATTGC AAAAATTGGTTGCTCATTCAAATTTTATGCCAATTACATTTTATCTAACATCTACATTGCCCTAATTTGT ACTGAAGTCCTTGTATATTGTGTTTGGTTGACTACTGGTAGCTGTGATGGGGGCTGCATTGTTTCACACT GAAGGGTTACATTTGCTTTAGCAAGTGTTTGGGGTCAAACTATGTCCAGGAACAAGGCTGGGAATACATA GCTAGGACACTGCTGTTGGAGGCCCCACCCCAGCCCCCGAACCTGCCCCTGGCCTCGCTCCAGGCTTGAG CTTCTTTATTAGCTTAGAAGGATGTCAACTTATCCAGGATATCATATTCAGAATATTCATCAAAATCATT TTAATTCTAGCATAGAATGGACTGAATTCCTGTTGGTATATTTGGCCTATCATCCTTTAAATGTCTCTGA TAATTTATTGATATCTATCTTTATAAAATAGAAAAAAAGTACTTTTGTGTAAAGATATTTGTCTTTAAAT TTAGTATTTCATATCAGCACATCAATGTATGTATAAATGTTACATGTTAATTGTGTAAAAGATTCTACAA TAAATTATTTTTACCACTTGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6822142 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6822142 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11610 same embryo
	EMAGE:11609 same embryo
	EurExpress:euxassay_006987 same experiment
	MGI:4825285 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source