EMAGE mouse gene expression spatial database

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EMAGE:11609

Rev1 REV1 homolog (S. cerevisiae) ( MGI:1929074)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006966. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006966_01	euxassay_006966_02	euxassay_006966_03	euxassay_006966_04

euxassay_006966_05	euxassay_006966_06	euxassay_006966_07	euxassay_006966_08	euxassay_006966_09

euxassay_006966_10	euxassay_006966_11	euxassay_006966_12	euxassay_006966_13	euxassay_006966_14

euxassay_006966_15	euxassay_006966_16	euxassay_006966_17	euxassay_006966_18	euxassay_006966_19

euxassay_006966_20	euxassay_006966_21	euxassay_006966_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11609_wholemount_strong.wlz

11609_wholemount_moderate.wlz

11609_wholemount_weak.wlz

11609_wholemount_possible.wlz

11609_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11609_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9154
Entity Detected:	Rev1, REV1 homolog (S. cerevisiae) ( MGI:1929074)
Sequence:	sense strand is shown >T9154 CTGCCGCGGAGGCGCGAGGCGGGCGCAGCGACTGAGGGAGCGTGGGCCGCGGTGCGGTGCGGTGCGGCTG GGCGGCCGCCTGACCGGTTGCCTTCCCCGGCGTTCCCCTCAGCCGCGGGGAGGACACTGAACGACGGCGG CGCCCGCGAGGCCCGAGGAAGCGGCTGGGCCCGGGCATGGTGGCCTGGGGCGGCGCGGAAGAAGCTCCAC CATGAGGCGAGGTGGATGGCGGAAGAGAACGGAGAATGATGGCTGGGAAAAATGGGGCGGGTACATGGCT GCCAAGGTCCAGAAGCTGGAGGAGCAGTTCCGCACAGATGCTGCTAACCAGAAGGATGGGACGGCGTCTG CAATATTTAGTGGAGTTGCCATCTATGTTAATGGATATACAGATCCTTCTGCGGAGGAATTGAGAAATCT AATGATGTTGCATGGAGGTCAATACCATGTGTATTATTCTAGATCCAAAACAACACACATTATTGCTACA AATCTTCCTAATGCCAAAATTAAAGAATTAAAGGGGGAAAAAGTAATACGACCAGAATGGATTGTGGAAA GCATCAAAGCTGGGAGACTCTTGTCCTCTGCTCCCTATCAGCTCTACACCAAGCCGTCGGCTGCACAGAA GAGTCTCAACTTCAATCCTGTGTGCAAACCTGAGGATCCTGGGCCAGGTCCAAGCAATAGAGCCAAACAG CTCAACAACAGGGTAAATCACATAATTAAGAAGATTGAGACAGAAAGTGAAGTGAAAGCAAATGGACTGA GCAGCTGGAATGAAGACGGTGTAAATGATGATTTTAGTTTTGAGGATCTGGAACATACATTTCCAGGAAG GAAACAGAATGGAGTAATGCATCCCAGAGATACCGCTGTCATTTTTAATGGACACACCCATAGTTCAAAT GGTGCCTTAAAGACACAGGATTGCTTGGTGCCTGTGGGCAACAGTGTTGCTAGCAGGCTATCTTTAGACT CCACCCAGGAGGAGAAGAGGGCAGAGAAGAGCAACGCGGACTTCAGAGACTGCACAGTGCAGCACTTGCA ACACAGCACCAGAAGTGCAGATGCTTTGCGGAGTCCACACAGAACTAATTCTCTCTCACCATCTTTGCAC AGTAACACTAAAATCAATGGTGCTCACCACTCCACTGTTCAGGGGCCTTCAAGCACAAAAAGCACTTCTG TTCTGACTCTTAGTAAAGTAGCGCCTTCAGTGCCATCCAAACCCTCGGACTGCAACTTCATTTCAGATTT CTATTCTCGTTCAAGACTGCATCACATCTCAACATGGAAGTGTGAATTGACTGAGTTTGTCAATACCCTA CAGAGACAAAGTAGTGGTATCTTTCCGGGAAGGGAAAAGTTAAAAAAAGTGAAAACAGGCAGGTCTTCTC TTGTTGTAACTGACACAGGCACTATGTCAGTGTTGAGCTCACCCAGACATCAGAGCTGTGTGATGCACGT TGATATGGACTGCTTCTTTGTATCAGTGGGTATACGAAATAGACCAGATCTCAAAGGAAAACCAGTGGCT GTTACAAGTAACAGAGGCACGGGAACGGCGCCTTTGCGTCCTGGTGCTAACCCGCAGCTGGAATGGCAGT ATTACCAGAATAGAGCCCTGAGAGGAAAAGCAGCAGATATACCAGATTCATCAGTATGGGAGAATCAAGA TTCTACACAAACAAATGGAATTGATTCTGTTCTGTCAAAAGCTGAGATTGCTTCTTGTAGTTATGAAGCC AGACAAGTTGGCATTAAGAACGGAATGTTTTTTGGATATGCTAAACAGCTGTGTCCTAATCTGCAAGCTG TTCCGTATGATTTTCATGCATGCAGGGAAGTTGCCCAGGCAATGTATGAAACACTGGCCAGCTACACACA CAGCATCGAGGCTGTCAGCTGCGATGAAGCACTGATTGACGTCACGGACATCCTTGCAGAGACGAAACTT TCTCCTGAGGAGTTTGCAGCTGCCCTCCGTATTGAAATCAAAGACAAGACCAAATGTGCTGCCTCTGTTG GAATTGGTTCTAATATTCTTCTTGCTAGAATGGCAACTAAGAAAGCAAAGCCAGATGGTCAGTACCACCT GCAGCCCGACGAGGTAGACGATTTCATCCGAGGGCAGCTCGTCACTAACCTGCCGGGAGTTGGACGTTCA ATGGAATCTAAGCTTGCATCTTTGGGGATTAAGACTTGTGGAGATTTGCAGTGTTTGACCATGGCAAAAC TCCAGAAAGAATTTGGCCCCAAGACAGGGCAGATGCTGTATAGGTTCTGCCGAGGTTTGGATGATAGACC TGTTAGAACAGAGAAGGAACGAAAATCTGTTTCAGCCGAGATCAACTATGGAATAAGGTTCACCCAGCCC AAAGAAGCAGAAGCTTTCCTTCTGAGTCTTTCAGAAGAAATTCAAAGACGACTTGAAGCTGCTGGCATGA AGGGCAAACGCCTGACTCTGAAAATCATGGTGCGGAAGCCAGGGGCCCCTATAGAAACTGCAAAATTTGG AGGCCATGGAATTTGTGATAACATCGCCAGGACTGTAACTCTGGACCAGGCAACAGATAGTGCAAAAATA ATTGGGAAAGCCACTCTAAACATGTTTCATACAATGAAACTAAATATATCGGATATGAGAGGGGTTGGTA TTCAAGTGAATCAGTTGGTTCCTGCTAATTCAAATCTTTCCACATGTTCAAGTCGTCCATCAGCTCAGTC AAGCCTCTTCTCTGGTCGGCCACATTCTGTCCGTGATCTCTTCCAACTTCAGAAAGCCAAGAAACCCACA GAAGAGGAACACAAGGAAGTATTTCTGGCTGCTGTGGATCTGGAAGTGTCCTCCACCTCTAGAGCTTGTG GTTTATTGTCTCCGCTCTCTGCACATCTGGCAGCTAGTGTTAGTCCTGACACTAACAGTGGAGAGTGTTC CAGAAAATGGAATGGCCTGCACTCTCCTGTCAGTGGACAGTCACGGCTGAACCTCAGTATAGAGGTCCCA TCACCATCCCAGATTGACCAGTCTGTTTTAGAAGCGCTCCCACTTGATCTCCGGGAACAAATAGAACAAG TTTGTGCCGCTCAGCAGGGAGAGCCACGTGGAAAGAAGAAAGAACCAGTAAATGGCTGCAGTTCAGGGGT GTTGCCACATCCTGTTGGAACAGTTTTATTACAGATCCCAGAGCCTCAAGAACCTTGTAATAGTGACAGC AAGATTAGTGTAATTGCCCTTCCAGCATTCTCACAAGTGGACCCTGATGTCTTTGCTGCCCTTCCTGCTG AGCTTCAAAAGGAGCTGAAAGCAGCATATGATCAAAGACAAAGGCAGGGAGAGGACACTACTCATCAGCA GCCAACCAGTACATCTGTGCCAAAGAATCCTTTACTTCAGCTAAAGCCACCAGCAATGAAAGACAAGAGG AACAAGAGGAAGAACCTCATTGGTTCTCCCAGAAAGAGTCCTTTGAAAAATAAGCTGCTTAGCAGTCCTG CAAAAACTCTGCCAGGGGCATATGGAAGCCCCCAGAAGTTAATGGATGGTTTTCTACAACATGAAGGAAT GGCTTCAGAGAGACCCCTGGAAGAAGTCTCGGCTTCTACCCCTGGTGCACAGGACCTATCTAGTTTACTG CCAGGCCAGAGCAGCTGTTTTAGACCAGCAGCACCCAACCTAGCTGGAGCTGTGGAATTCAGTGATGTGA AGACCTTGCTCAAAGAGTGGATCACTACTATCTCAGATCCGATGGAAGAAGACATCCTGCAGGTTGTGAG ATACTGCACTGACCTCATAGAGGAGAAAGACTTGGAAAAATTGGATCTGGTTATAAAATACATGAAAAGG TTGATGCAGCAGTCGGTGGAGTCTGTATGGAACATGGCGTTTGACTTTATTCTTGACAATGTTCAGGTGG TTTTACAGCAGACTTATGGAAGCACACTGAAAGTGACCTGACTGCTGTGCAGAGGGCCTGGGGCTCTCTG CGCTGTGCCAGCAGTGCTTGTGAGGTATTTGCAAAGTGCATGATAGTGATGCTCTGAGTTTTCATAATTC TACATTTCTTTTTAAGCAAGTGTTTTGTACATTTCTTTTCAAAAAAGTGCCAAATTTGTCAGTATTGCAT GTAAATAATTGTGTTAATTCTTTTACTTGTAGCATAGATTCTATTTACAAAACGTTTGTTTATAAAGTTT TATGGATTTTTACAGTGAAGTGTTTACAGTTGTTTAATAAAGAACTGTATGTATATTTTGTAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827108 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827108 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11610 same embryo
	EMAGE:11608 same embryo
	EurExpress:euxassay_006966 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source