EMAGE mouse gene expression spatial database

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EMAGE:11626

Anapc10 anaphase promoting complex subunit 10 ( MGI:1916249)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006622. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006622_01	euxassay_006622_02	euxassay_006622_03	euxassay_006622_04

euxassay_006622_05	euxassay_006622_06	euxassay_006622_07	euxassay_006622_08	euxassay_006622_09

euxassay_006622_10	euxassay_006622_11	euxassay_006622_12	euxassay_006622_13	euxassay_006622_14

euxassay_006622_15	euxassay_006622_16	euxassay_006622_17	euxassay_006622_18	euxassay_006622_19

euxassay_006622_20	euxassay_006622_21	euxassay_006622_22	euxassay_006622_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11626_wholemount_strong.wlz

11626_wholemount_moderate.wlz

11626_wholemount_weak.wlz

11626_wholemount_possible.wlz

11626_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11626_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6803
Entity Detected:	Anapc10, anaphase promoting complex subunit 10 ( MGI:1916249)
Sequence:	sense strand is shown >T6803 GAGAACCGGAATTGTGGCGAATCAGAAGGATTGTCATGGCATGGTAGAATGTATATTGCTTGTCTCTCTA TAGAATAATTTAAAAATGACCACACCGAACAAGACACCTCCTGGTGCTGATCCTAAGCAGTTGGAGAGGA CAGCAACAGTCAGGGAAATTGGGTCTCAGGCTGTTTGGTCACTCTCTTCCTGCAAACCAGGGTTTGGAGT GGATCAATTACGGGATGACAATCTAGAAACTTACTGGCAATCTGATGGCTCTCAGCCTCACTTAGTGAAC ATCCAATTCAGAAGAAAAACAACAGTGAAGACATTATGTATTTATGCAGACTATAAGTCTGATGAAAGCT ACACTCCAAGCAAAATCTCAGTCAGAGTAGGAAATAATTTTCACAACCTTCAAGAAATCCGGCAACTTGA ATTGGTGGAACCAAGTGGCTGGATTCACGTTCCTTTAACTGACAATCATAAGAAGCCAACTCGCACATTC ATGATACAGATTGCTGTTCTAGCCAATCATCAGAATGGAAGAGATACCCACATGAGACAAATTAAGATAT ACACACCAGTGGAAGAGAGCTCCATTGGGAAATTCCCTAGATGCACAACCATTGATTTCATGATGTATCG TTCAATACGGTGACTTTAAAATGGACAGAAGTGAACAGACGTTTGTTTTATCCTATCATTGGATAGTATA TCAAGTATCTTTGGTGAAAAGTCATGTTACTTTGCAATTTTACATACATTTAAAATAATTTAGATAAATA ACTTTTAGGTCATGTCTGTTTTTTATATAATAAAGCTCATATTTTATGTTGCTAACATGAATTTGAAGTC AATCTTTAAATTTTTCTTTATATTAAAAGAAAACTAGTTAAACTGAAAATTTCAAGATATATATACTATG TGTTTACTTTATTTATGGTGGTTATTTCATATAATGGTTGTTGGATATTGGGAGCAAAATGGAATTTTCA ATAGCACTGAAGTGGAAGCTTAAAGGTACTTTGGAATATTAGTTGTGTTGGATGGTGTTTTCCTGGAGCA GACACAGGTGAAAGACCAAGGAACATTTGGCTGAAACAGACCCAGGTATGTTCTGCTAAAGCAAGCATGT GAGAGGGCTCTGAGTGAAGACATTCATGTATTGGTCCTCCTTACATTGCATAGTTGAGCTGCATTTGTTG GGACTCTTAACACCAAAACCTTCTGGTGGTGTGCTACTGCTTCTTGTTGCTTCTGCAGATTTGGGCTGAT TGGCAGAGACCTGTCACCTGAGACATACTTAATGTGCTGAAGCAAAGCATGTGCTGAGACAATAACATGT GGAGGACACTTGATGTCTGGAGAGATGATAAATAGGACTCACAATGACGGAGGCTGAACTAGTCTTGCTT ATAGAGCTGTCTGTGCAACGCTTGAGAGGCACAGCTGAGAGCTTTTCCTGGTGTTCCTGTTGGCCATGGT CCTTCCTGTTGACTAGTGCCAGAGCTGAGGCCTGGCTGTCTCTGCTAGGTAGTGCCATCGCTGCTGATTG GTGTTTGCTATCCTGACTTCTGTGAACTGAACTGCTGATTTCCTGACAATGCAGATGGGAGTTGCTCTAA AGAACCATATCTTAAACAAATCTACTTCCCCTGTGTTCTATCCTTCCACTACTTCTGGTGGATGGAAGGC TAAAAGGAAGGTTAAAGCATTTAAGAACCCTCATTTAAAAAAAGGCTTGAAAAAATTAAAGTTACACATT AATTTTTTTTTTAGAGGAAATTAAATGTTTGTGTCCCCCCTCCCCCGAAACAATGATAATTCAATCTCTG GGTGCAACAACTAGAATCCTAATCTTGTAAGCCATATTAAATCTAAATCCTCAGGTGGCAAATCCTGGCA GATTCGCCATTTAAGCTGAGGACACTAGTAGCTACATCTTGCCTTCTCGATAACCCTGTCCAAAAAGCCC CTAACTCTCCTGCTTCCTCTCTTCCTCTGTCCAACCTTGAAGTCTCACCTACTTGCCCAGTGATTGGTCC CTGTATTCAGACAGCCCCTCCTGGGAAAGCAGAATTAACATCGTAAGTGGAATTAATACAGGCATCACCA GGGCCATCCACAACACTTTCCTTTTTCTGTACTAATAAAAAACAAAACTTTTCTCTCAAATATAAATTGA GCACAACTACTACCATTTTGTAATTTATAAAGTACAAGATAGACCTAACACCCAGTCAGTCCATCATTTT TGTCAATTAAATAGAACACTGTCATATTTCCTAACTTAAAAGACGTATAATTCTACACCTGACTTATGTC CTGGCTTTAGATTGTATGCCATCTGAAAACCATCCTCAAATCTGTTTTATCAATGTTAAATAGCCTGGGT TGGCTTTGAGACTATAAGTAGTCTTTCAACTCCATCAGAAATCCGAGAATGACCAACATTATCTGAAGAT AAAGGAAGCCTAACACAGCTTCCAGACCTGAGACAAGTTGTAGAGACAGCTGCCTACCCATATAGCCCCA GTAAGTCAGGAAGTTGGAGCATCAATCTTCATCCTCTGGCCCAGAATCATCTGACAGATCTTTGAAAAAC AGGAATTGTTAAGGATTAGCCTATTGTCTCAGCAGAACTAAGCTGTCCTAACCTGAACATTGTGTCCTTA GATAGCCAGTTTCTGCTCAGTGGCCACCTGGCCACAGTGTATCACTTCACTTGGAGGTATAGATGCTCTG TTGCTTTGAATCCATGAATGAGAAGCTGTTAGGAGATTATCTCAGCAAGTGAATAAATAACATTAAATGT CAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3710036 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3710036 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11625 same embryo
	EurExpress:euxassay_006622 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source