EMAGE mouse gene expression spatial database

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EMAGE:11630

Tmod4 tropomodulin 4 ( MGI:1355285)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006523. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006523_01	euxassay_006523_02	euxassay_006523_03	euxassay_006523_04

euxassay_006523_05	euxassay_006523_06	euxassay_006523_07	euxassay_006523_08	euxassay_006523_09

euxassay_006523_10	euxassay_006523_11	euxassay_006523_12	euxassay_006523_13	euxassay_006523_14

euxassay_006523_15	euxassay_006523_16

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11630_wholemount_strong.wlz

11630_wholemount_moderate.wlz

11630_wholemount_weak.wlz

11630_wholemount_possible.wlz

11630_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11630_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 11 12 13 14 15 16 weak expression: see section 05 06

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 11 12 13 14 15 16 weak expression: see section 05 06 07 08 09 10

lung

strong

regional

strong expression: see section 13 moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7964
Entity Detected:	Tmod4, tropomodulin 4 ( MGI:1355285)
Sequence:	sense strand is shown >T7964 GGCTGTGAGTGTCACACCGTGGGGAAGGGGTAGGGGCCACCATGTCATCCTATCAGAAGGAACTGGAGAA ATACAGAGACATCGACGAAGATGAGATCCTAAGGACCCTGAGCCCTGAAGAGCTAGAACAGCTGGACTGT GAGCTACAAGAAATGGATCCTGAGAACATGCTCTTGCCAGCTGGCCTGAGACAACGTGACCAGACAAAGA AGAGTCCAACAGGGCCACTGGACAGAGATGCCCTATTGCAGTACCTGGAGCAACAGGCGCTAGAAGTCAA AGAGCGTGATGACTTGGTGCCCTACACTGGCGAGAAGAAGGGAAAACCCTTTATTCAGCCTAAGAGAGAA ATCCCAGCACAGGAACAGATTACGCTGGAGCCTGAGCTGGAGGAAGCGCTGTCCCATGCTACAGATGCTG AAATGTGTGACATTGCAGCCATCCTGGGCATGTATACACTGATGAGCAACAAGCAATATTATGATGCCAT CTGCAGTGGAGAGATCTGCAACACAGAAGGCATTAGCAGTGTGGTACAGCCTGACAAGTATAAGCCAGTA CCAGATGAACCTCCAAATCCCACAAACATTGAGGAGATGCTAAAGAGGGTACGGAGTAATGACAAAGAGC TGGAGGAGGTGAACCTCAATAATATCCAGGACATCCCAATACCTGTGCTAAGTGACTTGTGTGAAGCAAT GAAAACCAATACCTATGTCCGGAGCTTCAGTCTGGTGGCCACAAAGAGTGGTGACCCTATCGCCAATGCG GTGGCTGACATGTTGCGTGAGAACCGCAGTCTCCAGAGCCTGAACATTGAGTCTAACTTCATCAGCAGCA CAGGGCTCATGGCTGTGCTAAAGGCAGTTCGGGAAAATGCTACTCTCACTGAGCTCCGTGTAGATAACCA GCGCCAGTGGCCTGGTGATGCGGTAGAGATGGAGATGGCCACTGTGCTAGAGCAGTGTCCGTCTATTGTC CGCTTCGGGTACCACTTTACACAACAAGGACCACGAGCTCGGGCAGCCCACGCCATGACCCGGAACAATG AGCTGCGTCGTCAGCAAAAGAAGAGATAACATCACCCTTCCTTTTACCAACAAGAGATGAGAGCCTGGGA CGCACAGATCCTCATCTCTCTCTCCTTTCCTGTAAATAAAATCCCTTTTGTTCACGCAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30291625 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30291625 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11628 same embryo
	EMAGE:11627 same embryo
	EMAGE:11629 same embryo
	EMAGE:11631 same embryo
	EMAGE:11632 same embryo
	EurExpress:euxassay_006523 same experiment
	MGI:4828826 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source