Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11654

Oprd1 opioid receptor, delta 1 ( MGI:97438)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11654 EMAGE:11654 EMAGE:11654 EMAGE:11654 EMAGE:11654
"Pseudo-wholemount" of euxassay_008431. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008431_01 euxassay_008431_02 euxassay_008431_03 euxassay_008431_04
EMAGE:11654 EMAGE:11654 EMAGE:11654 EMAGE:11654 EMAGE:11654
euxassay_008431_05 euxassay_008431_06 euxassay_008431_07 euxassay_008431_08 euxassay_008431_09
EMAGE:11654 EMAGE:11654 EMAGE:11654 EMAGE:11654 EMAGE:11654
euxassay_008431_10 euxassay_008431_11 euxassay_008431_12 euxassay_008431_13 euxassay_008431_14
EMAGE:11654 EMAGE:11654 EMAGE:11654 EMAGE:11654 EMAGE:11654
euxassay_008431_15 euxassay_008431_16 euxassay_008431_17 euxassay_008431_18 euxassay_008431_19
EMAGE:11654 EMAGE:11654 EMAGE:11654
euxassay_008431_20 euxassay_008431_21 euxassay_008431_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11654Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11654_wholemount_strong.wlz
11654_wholemount_moderate.wlz
11654_wholemount_weak.wlz
11654_wholemount_possible.wlz
11654_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11654_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35274
Entity Detected:Oprd1, opioid receptor, delta 1 ( MGI:97438)
Sequence:sense strand is shown

>T35274
CAGAGGACATAGGTTCGATTCCCAGCACCCACATAGTGGCTCACAGCCATCTGTAACCCCAGTCGCAGTC
AATCTAATGCTTTCCAACAACTGTGGGCACCAGACATGAACATGGTACACATACATACATATATACATAC
ATACAGGTGAAACACTCAGACACATAAAATAAATCTTCTTTTAAAAGTGGTGGCACATGCCTTTAATCCC
AGCACCTGGGAGGCAGAGGCCAGCCTGGTCTACATAGTGAGTTCCAGGATGCTCAGGGCTACATGGTGAG
ACCCTGTCTCAATAAAAGTGTGTGTTGTAGGGAGAGTAAATCGTGAGGCAGGGGGCTGAGGCCTCTGCTT
ACAGGTTAAATCCTGAAGCCTGAACATGGATGCTTTTGGGGTTCCTGGGGTACTAGTGTATGTGGCTCAG
TACCAGAGCACCGAGACCCTTTGTTTGAGCCCCCCCGTATGTGGTAACATGAATAAGGATTCTCACCAAA
TACTTGGACCCTTGATAAGAACCCGGAAGAAGACTCTTGCCCCTTCTCCCAGCGTCTGGCTGTCCATCAG
ATGCCTGGTGTCCATGACTCCCAGGGACTGGAACTGCCCCAGTCGGACTCTGCTTTGAAGGGATTGAGGG
AAGAAGGGCTTTTATAGCAGTGATGTCGGTGTGGCCCTTTGCTCTCTCCCATTTCTCCTGAGTCCCTCAT
TTGCTCTCGCTGCAGAGGGTGGCCCAGAAACTGATCCAGGCTCTCCTGGATCTCAGCTCCCCTGGATGTC
ATTGTTTGCCCTGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71192. Forward Primer - name:071192_F_cDNA_Oprd1, sequence:CAGAGGACATAGGTTCGATTCC; Reverse Primer - name:071192_N_SP6_cDNA_Oprd1, sequence:GACAGGGCAAACAATGACATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11653 same embryo
 EurExpress:euxassay_008431 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS