EMAGE mouse gene expression spatial database

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EMAGE:11659

Gnpat glyceronephosphate O-acyltransferase ( MGI:1343460)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008409. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008409_01	euxassay_008409_02	euxassay_008409_03	euxassay_008409_04

euxassay_008409_05	euxassay_008409_06	euxassay_008409_07	euxassay_008409_08	euxassay_008409_09

euxassay_008409_10	euxassay_008409_11	euxassay_008409_12	euxassay_008409_13	euxassay_008409_14

euxassay_008409_15	euxassay_008409_16	euxassay_008409_17	euxassay_008409_18	euxassay_008409_19

euxassay_008409_20	euxassay_008409_21	euxassay_008409_22	euxassay_008409_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11659_wholemount_strong.wlz

11659_wholemount_moderate.wlz

11659_wholemount_weak.wlz

11659_wholemount_possible.wlz

11659_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11659_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7138
Entity Detected:	Gnpat, glyceronephosphate O-acyltransferase ( MGI:1343460)
Sequence:	sense strand is shown >T7138 CCACGCGTCCGGGCGGTGCCCGGGGTCCCGTGCAGTGGCCGTACGGAGTGCCACTGTCCTGGGCAGACGG GAGCCGCCCCGTGCGAAAGAACCGCCCCCAGCGGCTGCACTACAGCCGTGTAGCAGACCAGCCCAGGCCC CGCCCGGGACGGCAGCCGCGCCATGGACGTTCCTAGCTCCTCCAGCTCCCGATTCTCGGTCGGCTCGGCC AGCCCTAGCAGTGTGCTCCTCTATGCGAAAGACCTCAAAAAGTGGGATGAGTTTGAAGATCTGTTAGAGG AGAGGAGACACATCAGTGACTTCAAATTTGCAATGAAGTGCTACACCCCGCCCTTGTATAGGGGGATCAC TCCCTGCAAGCCAGGGGACATCAAGTCCATTGTCCTCAGTTCTGAAGAGATCAACTATGTCATTAAGCAG CTTTCTAGGGAGTCGCTGACAGGTGTGGATGTCCTCCGAGAGGAAGCCAGTGAGATCCTCGAGGAGATGA GCCACAAACTGCGCATAGGAGCCATTCGGTTCTTTGCCTTTGTTCTGAGCAAAATCTTTAAACAGATCTT CTCAAAGGTGTGTGTGAATGAAGAAGGCATTCAAAAGCTACAACGCGCTGTCCAGGAGCACCCTGTGGTC CTGCTGCCCAGTCACCGCAGCTACATTGACTTCCTCATGCTGTCCTTCATTCTCTACAGCTATGACCTGC CTGTGCCAGTGATCGCAGCAGGAATGGACTTCCTGGGAATGAGAGTGGTCAGTGAGCTGCTCAGGATGTC TGGAGCCTTCTTCATGCGACGCACTTTTGGAGGAAATAAACTCTACTGGGCCGTATTCTCTGAATATGTG AAGACCATGCTGCGGTGTGGTTATGCTCCTGTTGAATTTTTCCTCGAAGGGACGAGGAGCCGAGCTGCCA AGACCCTGACTCCTAAGTTTGGTCTCCTGAATATTGTGATGGAACCATTTTTTAAAAGAGAAGTCTTCGA TACCTACTTTGTCCCAATTAGCATCAGTTATGATAAAATATTGGAAGAGAGTCTTTATGCATATGAGATC CTCGGAGTTCCTAAGCCGAAGGAGTCCACCACTGGATTGTTGAAAGCTAGACGGATTCTCTCTGAGAACT TTGGAAGCATCCATGTGTACTTCGGAGACCCTGTATCACTGCGTTCTCTAGCTGCAGGGCGGCTCAATCG GAACACGTATAACTTGGTGCCAAGATGCATCCCTCAGAAGCAGCCTGAGGACGTGCAAGCCTTTGTCACT GAGGTCGCCTATAAGATGCAGCTTCTGCAGATTGAAAACCTGGCTCTGAGCCCATGGCTCCTGGTGGTCA CCATTCTACTTCAAAACCAGCTATCCATGGACTTTGACGCCCTGGTGGAGAAGACTCTGTGGCTGAAGGG CGTAACCCAGGTGTTCGGCGGGTTCCTGCTTTGGCCTGATAACAAACTTCCTGAGGAAGTGGTCCAGTCC AGCATCCTCCTGCACTCTAACCTGGCCAGCCTTGTCAAAGACCAGGTGGTTCTGAAAATGAACTCTGGAA GCTCACAAGTGGTCAACGGGCTTGTGCCCGAACATATCGCCCTCCTCATGTGCTCTGCCTACAGGAACCA GCTGCTCAACATTTTTGCCCGTCCTTCCTTAGTCGCCCTGGCTTTGCACATGACACCTGGGCTCAGGAAA GAGGATGTCTTCAGTTGCTTTTCCTTCCTCCGCAATGTGTTTTCAGATGAGTTCATCTTCCTTCCAGGAA ACACACTCAGGGACTTTGAGGAAGGCTGTTACTTGCTGTGTAAAGCTGAGGCTATGCAGATGGCTGGGAA GGACATCATCCTCACAGACAAAGGGACTGCTGTGTTACAGTTCCTCACAAGCCTCTTTAAGCCTTTTGTG GAATCATATCAGTTACTTTGCAGATACCTCTTGCATGAAGAGGACTACTTTGGTGAGAAGGAATACTTGG TTGCAGCCAGGAAGTTCACCCGTCAGCTTCTGGATCAAGGCTCCTCTCAGTGTTACGATGCGCTGTCTTC TGAACTGCAGAAAAATGCCTTAGCAGCTTTCGTGAGACTGGGGGTGGTGGAAAAGAAGAAGATAGATAGT AAGTATGTGTATTATGTGAACGGGCCTGCCACAAGCAAGTTAGAAGAAATGCTCGGATGTAAGAAGCCAA TAGGAAAACCAGCCACTGCAAAACTATAACAATAATGGAGCAGTTGTAGAAAACACAGTTCCATAGTGAC AGTCACAAAGGACTTACTCTGCCACAAATGGGCACTGAGAGGAAGATGTGACGTCCGTCTCCTTGAGACC TCTGAGCCATGCTTCTGCCAGGAGGAGGAGAGCATGGGAGTGTCGGTGTGACCCTCCCACCGTGATGGCT GCTGGCCGGCATTCGTGGCACCGTGTTGTCAAATAAAGCAGTCTTGAAAACATTTCTTGGGCAGCAGAGC TCAGGCCCCTCCCCGTTTACTGCCACTCAGATTCCCACACCTACCCCACTCTCAGGCTGACCTCCGAGGT TTAGTACAGCAGCAGCATTGACCACTTATCTGTGCACTGAAGAAGGGACACTGGTGAGTGTTCCCACCCA CAGCTCCATGAGGGTCCCAATCAAACCACAGGGCACTAAGAACACTGTGTGTTGGGTGTGTATAAAACCA AGGACGTTCATGTCTAGATTAGCTCAAGTCTCCAGGACATTTTGTTCTGCAAAAGTACACCTTCCGAACC TGAAAAGCAGTCCAGCTCACTGTTCTCTGTCAGTGTTTTCAATAAACTGTTCTTACTAAACAAACAAAAG CTTTAAATCTGACACACTTCCATCATAACTATTTTGTATAAGGGACTCAACCTATATCTTTTTACTTCAA TTACGTTGTCTCAACCAAGAGCAAATTTGACAGAAGTGTGTAATAAATTATTTAGTTTACTTTAAACTTT AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4020505 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4020505 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11660 same embryo
	EMAGE:11657 same embryo
	EMAGE:11658 same embryo
	EMAGE:11656 same embryo
	EMAGE:11655 same embryo
	EurExpress:euxassay_008409 same experiment
	MGI:4825145 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source