EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11672

Cacnb2 calcium channel, voltage-dependent, beta 2 subunit ( MGI:894644)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008283. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008283_01	euxassay_008283_02	euxassay_008283_03	euxassay_008283_04

euxassay_008283_05	euxassay_008283_06	euxassay_008283_07	euxassay_008283_08	euxassay_008283_09

euxassay_008283_10	euxassay_008283_11	euxassay_008283_12	euxassay_008283_13	euxassay_008283_14

euxassay_008283_15	euxassay_008283_16	euxassay_008283_17	euxassay_008283_18	euxassay_008283_19

euxassay_008283_20	euxassay_008283_21	euxassay_008283_22	euxassay_008283_23	euxassay_008283_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11672_wholemount_strong.wlz

11672_wholemount_moderate.wlz

11672_wholemount_weak.wlz

11672_wholemount_possible.wlz

11672_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11672_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epithalamus

moderate

regional

moderate expression: see section 11 15

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 13 14 17 18 19 weak expression: see section 12 20

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 11

pons ventricular layer

moderate

regional

moderate expression: see section 11

midbrain ventricular layer

moderate

regional

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35117
Entity Detected:	Cacnb2, calcium channel, voltage-dependent, beta 2 subunit ( MGI:894644)
Sequence:	sense strand is shown >T35117 ATAACCACAGAGAGGAGACCCACAGCAGCAATGGCCACAGACACAGGGAGTCTCGCCACCGCTCTAGGGA CATGGGTCGAGACCAGGACCACAATGAGTGCATCAAACAACGAAGCCGGCATAAATCTAAGGATCGCTAC TGTGACAAGGAGGGGGAAGTAATATCCAAGAGAAGGAATGAGGCTGGCGAGTGGAACAGGGATGTATACA TCCGCCAATGACCGTGCGTGTCCCTACCCCCAAGTCTTTGTGTATCATCCTTAAGCAAAATCTTTGGGGG TTACATTGCAACCATATGTGATTTGTCTTGTCTATTTTGTATTGCTGTTGCTCCAATAGCAATTGCATGC AATCAAGTATTACTATACATTCTTTTGTAAGTACCACAGAAATTGGCCTAGTACAGCTGCAGACTATATC CTGAACAATTCAAAAGCTGTTTGGGTAGCTGCCATCTCAACAGAGTGGCTCCAGAAACGTAGTTACTATA TCCAGTGAGCCAGATGCAGCGCAGATAACAAGTGGGATTTCTCCGTATTTTCAATACCCAGATACCTGAC CTGTCATTCTTTCATGGACCACTGTTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91076. Forward Primer - name:091076_F_cDNA_Cacnb2, sequence:ATAACCACAGAGAGGAGACCCA; Reverse Primer - name:091076_N_SP6_cDNA_Cacnb2, sequence:GAAACAGTGGTCCATGAAAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11671 same embryo
	EMAGE:11674 same embryo
	EMAGE:11673 same embryo
	EurExpress:euxassay_008283 same experiment
	MGI:4823582 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source