EMAGE mouse gene expression spatial database

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EMAGE:11698

Atad3a ATPase family, AAA domain containing 3A ( MGI:1919214)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006914. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006914_01	euxassay_006914_02	euxassay_006914_03	euxassay_006914_04

euxassay_006914_05	euxassay_006914_06	euxassay_006914_07	euxassay_006914_08	euxassay_006914_09

euxassay_006914_10	euxassay_006914_11	euxassay_006914_12	euxassay_006914_13	euxassay_006914_14

euxassay_006914_15	euxassay_006914_16	euxassay_006914_17	euxassay_006914_18	euxassay_006914_19

euxassay_006914_20	euxassay_006914_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11698_wholemount_strong.wlz

11698_wholemount_moderate.wlz

11698_wholemount_weak.wlz

11698_wholemount_possible.wlz

11698_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11698_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9182
Entity Detected:	Atad3a, ATPase family, AAA domain containing 3A ( MGI:1919214)
Sequence:	sense strand is shown >T9182 GCGGCGCGTGGTGACTGCGAGGAGCCTCGCGAGTCACGCTCCGGTACGGTCCGCCGAGGGCGCGCGGAGC TTGCAGACCATGTCGTGGCTCTTCGGCATCAAGGGCCCCAAGGGCGAAGGCACAGGGCCTCCGCTGCCCT TGCCGCCCGCTCAACCCGGGGCGGAGGGCGGCGGTGACCGCGGGGCGGGAGACCGGCCATCGCCCAAGGA CAAATGGAGCAACTTCGACCCGACGGGCCTGGAACGTGCGGCCAAAGCGGCTCGCGAGTTGGAGCACTCG CGCCATGCCAAGGAGGCACTGAGTCTCGCACAGATGCAGGAGCAGACGCTGCAGCTGGAACAGCAGTCCA AGCTCAAGGAGTACGAAGCTGCCGTAGAGCAGCTGAAGAGCGAACAGATCCGTGTGCAAGCCGAGGAAAG AAGGAAAACCCTGACTGAAGAGACACGACAGCACCAGGCTAGGGCCCAGTACCAGGATAAGCTCGCTCGA CAGCGCTATGAGGACCAGCTGAAACAACAGCAACTTCTGAATGAAGAGAATTTAAGGAAACAAGAGGAGT CTGTGCAGAAGCAGGAGGCCATACGGCGAGCCACTGTGGAGCGCGAGATGGAGCTGAGGCATAAAAACGA GATGTTGCGGGTGGAAGCTGAAGCCCGAGCACGGGCCAAGGCTGATCGAGAGAATGCAGATATCATCCGG GAACAGATTCGACTCAAGGCTGCTGAGCACCGCCAGACCATCTTGGAGTCTATCAGGACAGCTGGCACCT TGCTTGGTGAAGGATTCCGTGCATTTGTGACAGACTGGGACAAAGTGACAGCTACGGTGGCTGGGTTGAC ACTATTAGCTGTTGGAGTCTATTCTGCAAAGAATGCTACTTCTGTTGCTGGTCGGTATATTGAGGCCCGA TTGGGAAAGCCGTCCTTGGTGAGAGAGACCTCCCGAATCTCAGTGCTAGAGGCATTGAGGCATCCCATCC AGGTCAGCAGGCGACTGGTCAGCAGACCCCAGGATGCATTGGAGGGCGTCATCCTCAGTCCTAGCCTGGA GGCACGGGTCCGAGATATTGCCATCGCAACAAGAAATACCAAGAAGAACAAAAGCCTGTATAGGAACGTT CTGATGTATGGGCCCCCGGGGACTGGCAAGACACTATTTGCCAAGAAACTTGCACTGCATTCAGGCATGG ACTACGCCATCATGACAGGCGGGGACGTGGCCCCAATGGGGCGGGAGGGTGTGACTGCCATGCACAAGGT CTTCGACTGGGCAAGCACCAGCCGACGAGGCCTCCTGCTCTTTGTGGATGAAGCAGACGCCTTCCTCAGG AAACGAGCGACTGAAAAGATAAGTGAAGACCTCAGGGCTACTCTGAATGCCTTCCTACACAGGACAGGAC AGCACAGTAGTAAGTTCATGCTGGTCCTGGCCAGTAACCAGCCTGAGCAGTTTGATTGGGCTATCAATGA CCGCATTGACGAGATGGTCTGCTTTGCCCTGCCACAGCGGGAGGAGCGAGAGCGCCTGGTGAGAATGTAT TTTGACAAGTATGTCCTTAAGCCGGCCACAGAAGGAAAGCAGCGCTTGAAGGTGGCCCAGTTTGACTATG GAAAGAAATGCTCAGAGGTTGCCCAGCTAACGGAGGGGATGTCAGGCCGGGAGATTGCTCAGCTTGCTGT GGCGTGGCAGGCCATGGCATATTCATCTGAGGATGGAGTCCTCACGGAAGCTATGATGGATGCCCGTGTG CAGGATGCTGTGCAGCAGCACCAGCAGAAGATGCAGTGGCTTAAAGTAGAGAGACCCGATTCCCAGACCA ACAAGCCGCCACATCCTTCACTCCTCAGCTGCTGAGCTGGACCTGGACACCTTGTACACCTGACTGGGGC AGCCAGTCTCTATGACCACACTGCTCCCTCCTTTCCTGTCTTCTTTGACCTCTGTAAGAGGTCAACATTG CTCCAAATGCCTCTGCGGAGTTGTGGGATACTGGAGGTGTCAGCTTCTCTGCCTCCCACACCTCCTCGTC TCTGACTCTGAGTGCTGGCAGAGGGAGGCATACTGCCTTCCTTTACCTCAGTACACTGGTGAGACATGTC TACAAGCCCAGAGCAGAGCAGAGAGTGTGCAGAGTGCCGTACCACAAGCCAGTGGCTATGCCAGCGGGAC TGGGGCCTACAGGGACCAGGCTAAAGGCACAGGGGGCCCTCAGCTTCCTGGACATTACTCCTACCATATA CACATCTGTGCCAAGAATTGGCCCAGTCTTTACTCGGTAGAGTGGATCTGGTGCTAGACAGCGCTTGGCC CGTGGCATCCAAGCGTCAGAACTACCGCCTTCCCTCTACTGAGTCCGGGCCTTTTAAATCCAGAATCGAA TAAAACTGTGACTGATAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6828037 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6828037 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11700 same embryo
	EMAGE:11701 same embryo
	EMAGE:11699 same embryo
	EMAGE:11702 same embryo
	EMAGE:11697 same embryo
	EurExpress:euxassay_006914 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source