EMAGE mouse gene expression spatial database

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EMAGE:11700

Rpn2 ribophorin II ( MGI:98085)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006884_01	euxassay_006884_02	euxassay_006884_03	euxassay_006884_04	euxassay_006884_05

euxassay_006884_06	euxassay_006884_07	euxassay_006884_08	euxassay_006884_09	euxassay_006884_10

euxassay_006884_11	euxassay_006884_12	euxassay_006884_13	euxassay_006884_14	euxassay_006884_15

euxassay_006884_16	euxassay_006884_17	euxassay_006884_18	euxassay_006884_19	euxassay_006884_20

euxassay_006884_21	euxassay_006884_22	euxassay_006884_23	euxassay_006884_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11700_wholemount_strong.wlz

11700_wholemount_moderate.wlz

11700_wholemount_weak.wlz

11700_wholemount_possible.wlz

11700_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11700_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9189
Entity Detected:	Rpn2, ribophorin II ( MGI:98085)
Sequence:	sense strand is shown >T9189 GCGCGGGGGAGAGGACGGCTTGGCAAGCAGGACCCGTGAGTGAGGTTCGCACGCCGCGACTCAACTGCCC CACACTGCGGCTGCCGGGCACGGCCCTGCCAAGCGGGACCGCCGCGACTGACCTTTGCCGGGAGCCCATT CTGGCAAGTGGCGTGTGCCGCGGGTCCCTGGCGTCCCCTCCGGCGCGCTCTTGGAGCCACTTCCCGGAGC GGAAGTCTGCCTGCTCGGGCTCCGGTGGGACCTGCTCGGAGGAATGGCGCCGCCGGGTTCAAGCGCTGTC TTCCTGTTGGCCCTGACAATCACTGCCAGCGTCCAGGCTCTGACCCCCACCCACTACCTCACCAAGCAGG ATGTGGAAAGGCTGAAGGCCTCACTGGACCGCCCCTTCACAGATTTGGAGTCTGCCTTCTACTCCATCGT GGGACTCAGCAGCCTTGGGGTACAGGTGCCAGATGTCAAGAAAGCGTGTACCTTCATCAAGTCCAACCTT GATCCCAGCAACGTGGATTCTCTCTTCTATGCAGCCCAGTCCAGCCAGGTCCTCTCAGGCTGTGAGATAT CTGTTTCAAATGAGACCAAAGAGCTCCTGCTGGCAGCAGTGAGTGAAGACTCCCCCATTGCCCAGATCTA CCATGCAGTTGCAGCACTCAGCGGGTTTGGGCTTCCCTTGGCATCCAATGAAGCCCTCGGTGCCCTTACC GCTCGCCTCGGCAAGGAAGAGACTGTGCTAGCAACGGTCCAGGCTCTGCAGACAGCGTCCCACCTCTCCC AGCAGGCTGACCTGAGGAACATTGTAGAGGAGATTGAGGACCTTGTTGCTCGGCTGGATGAACTAGGGGG TGTGTATCTGCAGTTTGAGGAAGGACTGGAGCTCACGGCGCTGTTTGTTGCTGCCACCTACAAGCTCATG GACCACGTGGGGACCGAACCGTCCATGAAGGAGGATCAGGTTATCCAGCTCATGAACACAATCTTCAGCA AGAAGAACTTTGAGTCCCTCTCAGAAGCCTTCAGTGTGGCCTCTGCTGCTGCTGCATTGTCCCAGAATCG CTACCACGTACCAGTGGTGGTTGTGCCCGAGGGCTCTACTTCTGACACTCAAGAACAGGCTATCCTGAGG TTGCAGGTCAGCAATGTTTTGTCTCAGCCTCTGGCTCAAGCTGCAGTGAAGCTGGAGCATGCTAAGTCGG CGGCTACCAGGGCTACCGTCCTGCAGAAGACGCCCTTTTCGCTTGTGGGGAATGTTTTTGAGCTAAACTT CAAGAATGTTAAACTTTCCAGTGGCTACTATGACTTCTCTGTCCGAGTTGAAGGTGACAGCCGTTACATT GCAAACACTGTAGAGCTTCGAGTCAAGATCTCCACTGAAGTTGGCATCACAAATGTTGATCTTTCCACTG TGGATAAGGATCAGAGCATTGCACCCAAAACTACCCGGGTGACCTACCCAGCCAAAGCCAAGGGTACATT CATCGCAGACAGCCATCAGAACTTTGCCCTGTTTTTCCAGCTGGTAGATGTGAACACCGGTGCGGAGCTC ACCCCTCACCAGACGTTTGTTCGACTTCATAACCAGAAGACTGGCCAGGAAGTGGTGTTTGTTGCTGAGC CAGATAACAAGAATGTATATAAGTTTGAACTGGACACCTCTGAAAGGAAGATTGAATTTGACTCTGCCTC TGGCACCTACACACTCTACTTAATCATCGGGGATGCCACATTGAAGAACCCAATCCTCTGGAACGTGGCT GACGTGGTTATCAAGTTCCCTGAGGAGGAAGCCCCCTCGACTGTGCTGTCCCAGAGCCTGTTTACCCCAA AACAGGAAATTCAGCACCTGTTCCGAGAGCCTGAGAAGAGGCCCCCCACAGTGGTGTCCAATACATTCAC GGCCCTCATCCTCTCGCCCTTGCTCCTGCTCTTTGCACTGTGGATCCGGATTGGAGCCAATGTCTCCAAC TTCACATTTGCTCCTAGCACAGTTATCTTCCACCTGGGACATGCTGCGATGCTGGGCCTCATGTATATCT ACTGGACTCAGCTCAACATGTTCCAGACCCTCAAGTACCTGGCTGTCCTGGGCACTGTGACGTTTCTGGC TGGCAACCGAATGCTGGCCCAGCATGCAGTTAAGAGAACAGCACATTAGTTCCAGAAGAAGTCTGAAGAC CCTGAAGGCTGAAATGAACATTTAACAAGGAGTGGGGACAGTTCATGGTGTGAAAGGAAGAACTGGGGAC ACAACAGGAGGGAGGAGATGCCTGTTCGTTAAAAAGAGAAAAGCTGAGCTGTGCTTACATGTTTACTTGT TTCTCACTTTTTGCTTCACAGAGCAGACATGTTTGGGCCCAGATTGTCTGTCCCTTTGTCTTGATGCCTG GCAAGATGCTGTGAATATCCCAGGCTACCCAGATGTTGTATTTGAAAAGTTGAAATCTGTAATTCATCAT CAGCTTTGGAATAAAGAGAATGGTGGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6829209 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6829209 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11701 same embryo
	EMAGE:11699 same embryo
	EMAGE:11702 same embryo
	EMAGE:11698 same embryo
	EMAGE:11697 same embryo
	EurExpress:euxassay_006884 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source