EMAGE mouse gene expression spatial database

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EMAGE:11701

Mcm7 minichromosome maintenance deficient 7 (S. cerevisiae) ( MGI:1298398)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006902. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006902_01	euxassay_006902_02	euxassay_006902_03	euxassay_006902_04

euxassay_006902_05	euxassay_006902_06	euxassay_006902_07	euxassay_006902_08	euxassay_006902_09

euxassay_006902_10	euxassay_006902_11	euxassay_006902_12	euxassay_006902_13	euxassay_006902_14

euxassay_006902_15	euxassay_006902_16	euxassay_006902_17	euxassay_006902_18	euxassay_006902_19

euxassay_006902_20	euxassay_006902_21	euxassay_006902_22	euxassay_006902_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11701_wholemount_strong.wlz

11701_wholemount_moderate.wlz

11701_wholemount_weak.wlz

11701_wholemount_possible.wlz

11701_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11701_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9192
Entity Detected:	Mcm7, minichromosome maintenance deficient 7 (S. cerevisiae) ( MGI:1298398)
Sequence:	sense strand is shown >T9192 CCGGCTACGTCCCGCCGCGCGTTCGTTTTCTGCTTCCCCAGAGAGAGATTTTTGAGCCCTTCAAGTCCTG CCACACCGTCCCCGGCAGCGATGGCGCTTAAGGACTACGCGATCGAAAAAGAAAAAGTTAAGAAGTTCCT CCAAGAGTTTTATTACGAGAATGAGCTTGGAAAGAAGCAGTTCAAGTATGGGACCCAGTTGGTTCATCTG GCTCATCGGGAACAAGTGGCACTGTATGTGGACCTGGATGACATAGCTGAAGATGACCCTGAGTTGGTCG ACTCAATTTGCGAGAATGCCAAGCGCTACTCAAGACTGTTTGGTGATGTTGTGCAAGAGCTTTTGCCTGA GTACAAGGAGAAGGAGGTGGTGAATAAGGATGTCTTAGATGTTTACATTGAACACCGGCTGATGATGGAG CAGCGAAGCAGAGACCCTGGGGCAGTCCGGAACCCCCAAAACCAGTATCCTTCTGAACTCATGCGAAGAT TTGAGTTGTATTTCCGAGGCCCAAGTAGCAGCAAGCCTCGAGTGATCCGGGAAGTACGAGCTGACTCTGT GGGGAAATTGTTAACTGTGCGTGGCATTGTCACTCGTGTGTCTGAAGTCAAGCCCAGGATGGTGGTGGCC ACATACACTTGTGATCAGTGTGGGGCAGAGACCTACCAGCCAATCCAGTCTCCCACTTTCATGCCTCTGA TCATGTGCCCCAGCCAGGAGTGCCAGACCAATCGCTCAGGGGGGCGGCTGTACCTACAGACTCGTGGCTC CAAATTTGTTAAATTCCAGGAAATGAAGATTCAAGAGCATAGTGACCAAGTTCCTGTGGGAAACATCCCT CGTAGCATCACAGTGGTTCTAGAAGGTGAGAACACAAGAATTGCCCAGCCTGGTGACCATGTCAGCGTCA CTGGCATCTTCTTGCCAGTCCTGCGCACAGGGTTCCAACAGATGGCACAGGGCTTACTCTCAGAAACTTA CCTGGAAGCCCACTGGATTGTGAAGATGACAAAGAGTGACGATGATGTCTCTGGGGCCGGAGAGCTGAGC TCAGAGGAGCTGAAGCAGATTGCAGAGGAGGATTTCTATGAGAAGCTGGCCGCTTCTATTGCTCCGGAGA TCTATGGACATGAAGATGTGAAGAAGGCGCTGCTGCTGCTTCTGGTGGGTGGTGTGGACCAGTCTCCTCA AGGCATGAAGATCAGAGGAAACATCCACATCTGCCTTATGGGAGACCCTGGTGTGGCCAAATCTCAGCTC CTATCTTACATCGACCGTCTGGCACCGCGAAGTCAGTACACAACAGGCCGTGGCTCCTCTGGTGTAGGGC TCACAGCAGCTGTGTTGAGAGACTCTGTGAGTGGAGAACTGACTTTAGAGGGCGGTGCCCTGGTGCTGGC TGACCAGGGCGTGTGCTGCATTGATGAGTTTGACAAGATGGCCGAGGCTGACCGCACAGCCATCCATGAG GTTATGGAGCAGCAGACCATCTCCATCGCCAAGGCGGGGATTCTCACCACGCTCAATGCCCGATGCTCCA TCCTGGCTGCGGCCAATCCTGCCTACGGCCGATATAATCCCCGCCGCAGTCTGGAGCAGAACGTCCAGCT TCCGGCTGCTCTGCTTTCTCGATTCGACCTCCTCTGGCTGATTCAAGACCGGCCCGACAGAGACAATGAC CTCCGGTTGGCCCAGCACATCACCTATGTCCACCAGCACAGCCGGCAGCCCCCTGCCCAATTTGAACCTT TGGACATGAAACTTATGAGACGGTACATAGCCATGTGCCACGAGAGACAGCCCACAGTGCCTGAATCTCT GGCTGACTACATCACCGCAGCATATGTGGAGATGAGGCGAGAGGCCCGGGCCAGTAAGGATGCCACCTAT ACTTCTGCCCGGACCCTGCTGGCCATTCTCCGACTTTCCACTGCTCTGGCTCGACTTCGGATGGTAGACA TTGTGGAGAAGGAAGATGTAAATGAAGCCATAAGGTTGATGGAGATGTCGAAGGACTCCCTTCTAGGTGA AAAGGGACAGACAGCTAGGACCCAGAGGCCAGCAGATGTGATATTTGCCACCATCCGTGAATTGGTGTCA CGGGGGCGGAGTGTCCACTTTTCTGAGGCTGAACAGCGCTGCATATCTCGTGGCTTCACACCAGCCCAGT TCCAGGCAGCTCTGGATGAATATGAGGAGCTAAATGTCTGGCAGGTTAATACTTCTCGGACACGGATCAC CTTTGTCTGATAGCCAGTTTTTACACCCTCCTGCCTACACCAGAGGGCATGCTGAATGCCCCCTCAAGAA AAAATCTCATTCTCCCATGCTGCACTTTTTTATTCTGCCAATAAAGTGTTTTGATTGCCAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6829737 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6829737 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11700 same embryo
	EMAGE:11699 same embryo
	EMAGE:11702 same embryo
	EMAGE:11698 same embryo
	EMAGE:11697 same embryo
	EurExpress:euxassay_006902 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source