EMAGE mouse gene expression spatial database

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EMAGE:11702

Adcyap1 adenylate cyclase activating polypeptide 1 ( MGI:105094)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006913. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006913_01	euxassay_006913_02	euxassay_006913_03	euxassay_006913_04

euxassay_006913_05	euxassay_006913_06	euxassay_006913_07	euxassay_006913_08	euxassay_006913_09

euxassay_006913_10	euxassay_006913_11	euxassay_006913_12	euxassay_006913_13	euxassay_006913_14

euxassay_006913_15	euxassay_006913_16	euxassay_006913_17	euxassay_006913_18	euxassay_006913_19

euxassay_006913_20	euxassay_006913_21	euxassay_006913_22	euxassay_006913_23	euxassay_006913_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11702_wholemount_strong.wlz

11702_wholemount_moderate.wlz

11702_wholemount_weak.wlz

11702_wholemount_possible.wlz

11702_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11702_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

medulla oblongata basal plate marginal layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

pons mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

strong

homogeneous

strong expression: see section 04 05 18 19 20 21

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 05 06 17 18

trigeminal v ganglion

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

strong

homogeneous

strong expression: see section 07 16 17

trigeminal v nerve

moderate

regional

moderate expression: see section 09 17

spinal cord mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 15 16 17

neural retina

strong

regional

strong expression: see section 01 02 03 23 24

tongue

moderate

spotted

moderate expression: see section 14 15 weak expression: see section 12

midgut

moderate

spotted

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

mandible

strong

regional

strong expression: see section 13 14

palatal shelf

strong

regional

strong expression: see section 10 14

tail mesenchyme

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9193
Entity Detected:	Adcyap1, adenylate cyclase activating polypeptide 1 ( MGI:105094)
Sequence:	sense strand is shown >T9193 GTTTTGTGCGTCTACAAAGTTTTGAGCGGAATTTTAGCCTCGGCAAACAAGTCTCCCCAGCTCCTTCAGC TAATTCCCGCGGCTTCTCTCCAGACACCAGCTCCAGACAGTGACTGATGCTTCTCGGGTTGTGATTCCAG CGCAGAAACTCGAAGGAGCCTTTGCCCGCTGTCCTACTTAGTCAACCCTTTCCTAGACACCAATGACCAT GTGTAGCGGAGCAAGGCTGGCCCTGCTGGTGTATGGGATAATAATGCATAGCAGTGTCTCCTGTTCACCT GCCGCCGGACTCAGCTTCCCTGGGATCAGACCAGAAGACGAGGCTTACGACCAGGACGGAAACCCGCTGC AAGACTTCTATGACTGGGACCCTCCCGGCGTGGGGAGCCCCGCCTCCGCGCTGCGTGACGCTTACGCCCT TTACTATCCAGCGGACAGGAGAGATGTCGCCCACGAAATCCTTAACGAAGCCTATCGAAAAGTCTTGGAC CAGCTGTCCGCCAGGAAGTACCTGCAGTCGGTCGTGGCCAGGGGCGCGGGGGAGAACCTAGGCGGCAGCG CGGTGGACGACCCGGCGCCCCTTACCAAACGCCACTCGGACGGCATCTTCACAGATAGCTACAGCCGCTA CCGAAAACAAATGGCTGTCAAGAAATACTTGGCGGCCGTGCTAGGGAAAAGGTATAAACAGAGGGTTAAA AACAAAGGACGCCGAATAGCATACTTGTAGCAGATCAGCTGCCGGCTACCTTGTGTATAAAATGAAAAGT CGTTTTCCAAATTGACTGACCAGTCATCACTCGTGTTCTTTCCAAACATGTATTTATGTATCAAGTAAAG CCATTAAATGACTATTTTGATAATAATATTGTTTTTCTTTTTATGAAGCACTAGAGAATGCACAGATATA CTTTGTGGACCAATTATTGATATATATTATAAGTATATATATTAAGAATATATATAAGTATAACAGAGAG CAATTAAGATGGGTGCACAAGGATTGAAAATTCGCCTGAGCTGTTTATGTTTTTATATAAAGTAAATAGA GAAAATAGACAACCATTGTTTTGAATATTACTCCTATTTTTGTAAACTGGAATTAAAGGATAGTATTTTT ATCCACAACCGTCTTGAAGATACCAATAATGGCCATTTGTACAAAAAACAATGATGCCCTGCTCCAGGGG AATTCTGAGGTAATGACTTGGGGAATTGCTGAAGGGCTTTCTTTCCCTCTGAGTCTGGGGCAGGCTGCTT GAACCCCAGCCTAACTAACTCAAGTGGGCATTGTCCCACTGGTTGCAGGGGCAATTCCAACAATTTCAGT TTCTTTGATTATGTGTATTTGTCTCTCCTCAGACTCTCAGCCCAGAAGGAAATTCTAATTAAACAACAGC TCTATCCAAATTGTGCTTCTCCCCAGAACTCATGTCATTCCCTGATAGAAGAGTTGAGGAACTGTACAGA AGAGACAGGCTTGGAGAGAGAGCTCTCTTTTCTGTACTTCCTGATTCTCCAGGGAACAGACTATTCTAAG GCTAGGGCAATTGGAACAAAGTGAAAGATATATAAGGGATTGGTAAAGGCAGAACATGGGGATTTGAGAT TTGAGAGTTGCCTCAGGTCTGAGAATCTGGGGGCAAGTCTAGCTCCTCTGTAGGTTCCACTGCCTGACAG ATCAGGTGCTGGTGTTGGAATGAATGCAAAGTACAATGTGTTTTTCTCCAGTGCTGTTCATGCTTTTCAT GTTGTGAAATGGCCAGGATCCTCCCCTTTGAACACTGTTCTGCAGAAGCCAGCTCTGTTCTTTGTGGATT TTCTGGAGACCCTCCTTCCTACCCTTGCCCTCATGCATTGTTTTAGAGTCATTTGCCATTTTCCACTCAC TTATCTTAAATTTGTGAATGCTAGTTATTTTTTGTTGTTGTTTGATGCAAGCAGTTACTGTGAAGTTTAA GAACCCCTGTGTAGCTTCCACAGAGAAATTATGCACTAAATATGAACCTTTTGTTTCTTGTTTATTGAGT TTGTAGGTAAAAATGTATTTTTCTATATTATGGCTTATTGCTTAGTAAAATTTATTTCATAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6829765 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6829765 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11700 same embryo
	EMAGE:11701 same embryo
	EMAGE:11699 same embryo
	EMAGE:11698 same embryo
	EMAGE:11697 same embryo
	EurExpress:euxassay_006913 same experiment
	MGI:4823000 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source