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EMAGE:11709

Immt inner membrane protein, mitochondrial ( MGI:1923864)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11709 EMAGE:11709 EMAGE:11709 EMAGE:11709 EMAGE:11709
"Pseudo-wholemount" of euxassay_010967. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010967_01 euxassay_010967_02 euxassay_010967_03 euxassay_010967_04
EMAGE:11709 EMAGE:11709 EMAGE:11709 EMAGE:11709 EMAGE:11709
euxassay_010967_05 euxassay_010967_06 euxassay_010967_07 euxassay_010967_08 euxassay_010967_09
EMAGE:11709 EMAGE:11709 EMAGE:11709 EMAGE:11709 EMAGE:11709
euxassay_010967_10 euxassay_010967_11 euxassay_010967_12 euxassay_010967_13 euxassay_010967_14
EMAGE:11709 EMAGE:11709 EMAGE:11709 EMAGE:11709 EMAGE:11709
euxassay_010967_15 euxassay_010967_16 euxassay_010967_17 euxassay_010967_18 euxassay_010967_19
EMAGE:11709 EMAGE:11709
euxassay_010967_20 euxassay_010967_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11709Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11709_wholemount_strong.wlz
11709_wholemount_moderate.wlz
11709_wholemount_weak.wlz
11709_wholemount_possible.wlz
11709_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11709_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
axial musculature
strong strong
regionalstrong expression: see section 04 05 06 17 18 19 20 21
adrenal gland
strong strong
regionalstrong expression: see section 08 09 10 15 16 17
thymus primordium
strong strong
regionalstrong expression: see section 12 13 14 moderate expression: see section 11
submandibular gland primordium
strong strong
regionalstrong expression: see section 06 07 08 09 10 15 16 17 18
pancreas
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14
thyroid gland
strong strong
regionalstrong expression: see section 12 13 14 moderate expression: see section 11
vibrissa
strong strong
regionalstrong expression: see section 07 08 09 20 21
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 08 09 15
facial vii ganglion
strong strong
regionalstrong expression: see section 05 06 18 19 20
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 06 07 16 17
trigeminal v ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 08 16 17 18 19 20 21
vagus x ganglion
strong strong
regionalstrong expression: see section 07 08 16
dorsal root ganglion
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 15 16
stomach
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09
midgut
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18
liver
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
metanephros
strong strong
regionalstrong expression: see section 07 08 09 10 15 16 17 18 19
seminiferous cord
strong strong
regionalstrong expression: see section 06 07 18 19 20
left lung
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11
right lung
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36511
Entity Detected:Immt, inner membrane protein, mitochondrial ( MGI:1923864)
Sequence:sense strand is shown

>T36511
AGAATTAGAGTTTCGTCGTCGCAGTCAAGAACAAATGGAAAGCTTTACTCTGGACATAAATACTGCCTAT
GCCAGGTTGAGAGGGATTGAGCAGGCTGTTCAGAGTCATGCAGTTGCTGAAGAAGAAGCCAGAAAAGCCC
ACCAGCTCTGGCTTTCAGTGGAGGCATTAAAGTACAGCATGAAGACCTCATCTGCAGAAATGCCTACCAT
CCCATTGGGGAGTGCAGTTGAGGCCATCAGAGTCAACTGTTCTGATAATGAATTTACCCAGGCTTTAACT
GCAGCCATTCCTCCAGAGTCCCTGACCCGGGGGGTATACAGTGAAGAGACCCTAAGAGCCCGTTTCTATG
CTGTTCAAAAACTAGCCCGAAGGGTAGCGATGATTGACGAAACCAGAAATAGCCTGTACCAATACTTACT
TTCCTACTTACAGTCCCTACTCCTGTTCCCGCCTAAGCAACTGAAGCCCCCGGCGGAGCTCTACCCTGAA
GATATAAATACATTTAAATTGCTCTCATATGCCTCCTATTGTATTGAGCATGGTGACCTGGAGCTGGCAG
CAAAGTTTGTCAATCAGCTCAAAGGGGAGTCCAGAAGAGTGGCACAGGACTGGCTGAAGGAGGCCCGAAT
GACCCTAGAAACTAAGCAGATAGTGGAGATCCTGACTGCATACGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66055. Forward Primer - name:066055_F_cDNA_Immt, sequence:AGAATTAGAGTTTCGTCGTCGC; Reverse Primer - name:066055_N_SP6_cDNA_Immt, sequence:GGCGTATGCAGTCAGGATCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11713 same embryo
 EMAGE:11711 same embryo
 EMAGE:11710 same embryo
 EMAGE:11712 same embryo
 EurExpress:euxassay_010967 same experiment
 MGI:4825586 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS