EMAGE mouse gene expression spatial database

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EMAGE:11729

Ephb4 Eph receptor B4 ( MGI:104757)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006536. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006536_01	euxassay_006536_02	euxassay_006536_03	euxassay_006536_04

euxassay_006536_05	euxassay_006536_06	euxassay_006536_07	euxassay_006536_08	euxassay_006536_09

euxassay_006536_10	euxassay_006536_11	euxassay_006536_12	euxassay_006536_13	euxassay_006536_14

euxassay_006536_15	euxassay_006536_16	euxassay_006536_17	euxassay_006536_18	euxassay_006536_19

euxassay_006536_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11729_wholemount_strong.wlz

11729_wholemount_moderate.wlz

11729_wholemount_weak.wlz

11729_wholemount_possible.wlz

11729_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11729_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6802
Entity Detected:	Ephb4, Eph receptor B4 ( MGI:104757)
Sequence:	sense strand is shown >T6802 CTCGGTTCCCACTTTGGTTTCCACCTTCCTTGACCGCGACCGGCGCACTAACCCCGGCGGCGCGACATCC ACCAACCACTCCAGGGAGCGAGAGAGACCGCGAGCAATCGGCCCACCCCAGCCAGCCAGGGCGGGACCCC CGAAGCCCCGAAGGGACCCCTAACTCTCAGCCACGTCCCGATCTCCGCCCGCCCAGCGACGGCCACTGCC AGGACGTCCCCCCTCAAGGCTGGCCTCCCCGCGCGCGCGCGCTGCGGCGGGGACGTGGGGGCCGCACCTG AGCGCGGGTGCCTGGCGCGCCCGCATGGGCCATCGTTGAGAGGCCCTCGACGGAAAGTCCCAGCAGCAGA GTCGGTGTGCACCCGCCCAGGGGAGGGTCAGACCTGGGGGGGCCAGGGCCCCCCAAACTCGGATCGCATT CAGCCAAAGTGAGGCGGCGCCATGGAGCTCCGAGCGCTGCTGTGCTGGGCTTCCCTCGCCACTGCTTTAG AAGAGACCCTGTTGAACACAAAACTGGAAACGGCGGATCTGAAATGGGTGACTTACCCTCAGGCAGAGGG CCAGTGGGAGGAGCTAAGCGGCCTGGATGAGGAACAGCACAGCGTCCGCACCTATGAGGTGTGCGACATG AAGCGTCCAGGGGGCCAGGCTCACTGGCTGCGCACTGGCTGGGTCCCAAGGCGAGGTGCTGTCCACGTGT ATGCCACGATACGCTTCACCATGATGGAATGCCTGTCCCTGCCGAGGGCCAGTCGCTCCTGCAAGGAGAC ATTCACTGTCTTCTATTACGAGAGCGAAGCTGATACGGCCACGGCCCATACGCCCGCCTGGATGGAGAAC CCCTACATCAAGGTGGACACAGTGGCCGCAGAACATCTGACTCGGAAGCGCCCTGGAGCTGAAGCCACAG GGAAAGTTAATATCAAGACGCTGCGCCTGGGTCCTCTCAGCAAAGCTGGCTTCTACCTGGCTTTCCAGGA CCAAGGAGCCTGCATGGCTCTGCTCTCCCTGCATCTCTTTTACAAGAAGTGCTCCTGGCTGATCACGAAC TTGACCTACTTCCCCGAGACGGTGCCTCGGGAGCTCGTGGTGCCGGTGGCAGGTAGCTGCGTGGCCAACG CGGTCCCTACCGCCAACCCCAGCCCCAGCCTCTACTGCCGGGAAGATGGTCAATGGGCTGAGCAGCAGGT CACGGGCTGCAGCTGCGCGCCAGGGTACGAGGCTGCGGAAAGCAACAAAGTATGCAGAGCCTGTGGCCAG GGAACCTTCAAGCCCCAAATAGGAGACGAGTCCTGCCTGCCGTGCCCAGCCAACAGCCACTCGAATAACA TTGGGTCTCCTGTCTGCCTGTGTCGAATTGGGTATTACCGGGCCCGCTCAGACCCCCGGAGTTCACCTTG CACTACCCCACCCTCTGCTCCAAGAAGCGTGGTTCACCATTTGAATGGTTCCACCCTGCGCCTGGAATGG AGTGCTCCCCTTGAGTCCGGAGGCCGAGAGGACCTCACTTATGCTGTTCGCTGCCGAGAGTGCCGTCCTG GGGGTTCCTGCTTGCCCTGTGGGGGCGACATGACCTTCGACCCCGGTCCTCGAGACCTGGTTGAGCCCTG GGTGGCAATCCGAGGGCTGCGTCCTGATGTCACCTATACCTTTGAGGTTGCTGCTTTGAATGGTGTGTCT ACCTTAGCCACTGGACCACCTCCTTTTGAGCCTGTCAATGTCACCACTGACCGTGAGGTGCCTCCTGCAG TGTCTGACATCCGAGTGACTCGGTCGTCACCCAGCAGCTTGATCCTGTCATGGGCTCTCCCCAGAGCACC CAGTGGGGCCGTGCTGGACTACGAGGTCAAGTATCATGAGAAGGGCGCAGAGGGCCCCAGCAGTGTTCGT TTCCTGAAGACATCAGAAAACCGAGCTGAGCTCCGGGGGCTGAAGCGGGGAGCCAGCTATCTGGTCCCGG TACGCGCACGGTCCGAGGCTGGCTACGGTCCCTTCGGCCAGGAGCATCACAGTCAGACTCAACTGGATGA GAGCGAGAGCTGGCGGGAGCAGCTGGCCCTGATTGCAGGCACTGCGGTTGTGGGTGTGGTCCTGGTCCTG GTGGTCGTCATCATTGCAGTTCTCTGCTTCAGGAAGCAGAGCTATGGGAGGGAAGTTGAGTACTCGGATA AGCATGGGCAGTATCTCATCGGGCACGGTACCAAGGTCTACATTGATCCTTTTACTTACGAAGACCCTAA TGAGGCAGTGAGGGAATTTGCCAAAGAGATCGATGTCTCCTATGTCAAGATTGAAGAGGTAATTGGTGCA GGTGAGTTCGGCGAGGTGTGCCGGGGTCGGCTGAAGGCACCAGGGAAAAAGGAGAGCTGTGTGGCCATCA AGACTCTGAAGGGTGGCTACACCGAGCGCCAGAGGCGTGAGTTCCTGAGCGAGGCCTCCATCATGGGCCA GTTCGAGCATCCCAACATCATCCGCCTCGAGGGCGTGGTCACCAACAGTGTGCCGGTTATGATCCTCACG GAATTCATGGAGAACGGAGCCCTGGACTCCTTCCTGCGGCTGAACGACGGGCAGTTCACAGTCATCCAGC TGGTGGGCATGCTGAGGGGCATCGCCTCGGGCATGCGGTACCTGGCTGAAATGAGCTATGTCCACCGAGA CCTGGCTGCTCGGAACATCTTGGTCAACAGTAACCTGGTCTGCAAGGTGTCCGACTTTGGCCTCTCCAGA TTCTTGGAGGAGAACTCCTCTGATCCCACCTACACAAGTTCCCTGGGAGGCAAGATTCCCATCCGATGGA CCGCCCCTGAAGCCATTGCCTTCAGGAAGTTCACCTCTGCCAGTGATGCCTGGAGCTATGGGATCGTCAT GTGGGAGGTCATGTCTTTTGGGGAACGGCCATACTGGGACATGAGCAACCAGGATGTGATCAATGCCATT GAACAGGACTACCGGCTGCCTCCTCCTCCAGACTGCCCCACCTCCCTCCACCAGCTCATGCTGGACTGTT GGCAGAAGGACCGGAATGCCCGGCCCCGCTTTCCCCAGGTGGTCAGCGCTCTGGACAAGATGATCCGGAA TCCCGCTAGCCTCAAAATCGTGGCCAGGGAGAATGGCGGGGCCTCACATCCACTCTTGGACCAACGGCAG CCTCACTACTCTGCTTTCGGTTCTGTGGGCGAGTGGCTTCGAGCCATCAAGATGGGAAGATACGAGGAAA GTTTTGCAGCGGCTGGATTCGGCTCCTTTGAGGTGGTCAGTCAGATCTCTGCCGAGGACCTTCTCCGAAT TGGAGTCACTCTGGCAGGACACCAGAAGAAAATCTTGGCCAGTGTGCAGCATATGAAGTCCCAAGCTAAG CCAGGAGCCCCTGGTGGGACAGGGGGACCAGCCCAGCAGTTCTGACCTCCAAGGACTCACCACCGTGGCA GATTCTTCTTTCCGGGAGGCAGAGTTGGGTGGGGACTCACAAGATGACCCCCTCCCCCTCGTCACAGCCT TCCCATTGGATTGCACTTTGAACAGAGGGGGTCGGAGACACAGGATTTGGGGAACCGTGCCATATGGGAT CATACATGTGCCCTCCAGGCGGGGAACCCCAAACTCAGAGTGAGTCTTTCCCTCAAGACTGGGCAAAGAA ACATCCCTACGTCTCTAACCTCCCATCTTCCCAGAGGGCTCTCTCCCCAAGCGCCTTCCACCTCAACGGG CATGTCCCTGCAGACCAAAGAGAAAGGGTGACCAGCCTGCCAACTTGGGAGTGGAAAATGCCGTCCCAGG AGGCAGGAAGGGGCTGTCAGGACCCGGTGATGTAATCATTGGGTTTTGATGTCCTGACTTGCTGTCACCA CCAAAGGCAATCATTTTTCCCTTGTAAATGCCCCTCCCCTCATCTGCCTTCATATTGAAGGTTCTGAAGT TTTACTGTTTTTTATTTTGTTAATTTTTTCCTCCTTCCCCCCTCCCTCCCCTTCTTGTCCAGATTTTGTG TGTTAAAGGGCACCTGGTTCCACTATCTCCTGTTGGGAACAAGGACCCATCGATATGTTCTAGAACAGTG CCTTGGAAATGCCACAGCCTTAGACAGTTCCTTCTTCCTTTCCTCCCCCCAAGTGCCCCCAAGCTGTGTT GTGTGGAGGGAAGGGGGGTGATGTTGTAAAAGGGACAGGAGGTTGGTGGTGGTCAACAGAGGCAGACAAC GGTGCTTGGAAGGGGATTCTTAAATTATATTTAAAAAGAAACTTTTTTTTTTTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3709926 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3709926 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11730 same embryo
	EMAGE:11732 same embryo
	EMAGE:11731 same embryo
	EurExpress:euxassay_006536 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source