EMAGE mouse gene expression spatial database

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EMAGE:11734

Smyd3 SET and MYND domain containing 3 ( MGI:1916976)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008432. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008432_01	euxassay_008432_02	euxassay_008432_03	euxassay_008432_04

euxassay_008432_05	euxassay_008432_06	euxassay_008432_07	euxassay_008432_08	euxassay_008432_09

euxassay_008432_10	euxassay_008432_11	euxassay_008432_12	euxassay_008432_13	euxassay_008432_14

euxassay_008432_15	euxassay_008432_16	euxassay_008432_17	euxassay_008432_18	euxassay_008432_19

euxassay_008432_20	euxassay_008432_21	euxassay_008432_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11734_wholemount_strong.wlz

11734_wholemount_moderate.wlz

11734_wholemount_weak.wlz

11734_wholemount_possible.wlz

11734_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11734_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9258
Entity Detected:	Smyd3, SET and MYND domain containing 3 ( MGI:1916976)
Sequence:	sense strand is shown >T9258 AGACTCAAGCTGAGGGACGCAGTTCGGATCTGAGGTGTGGATCTGAGGAAGGATGGAGGCACTGAAGGTG GAAAAGTTCACGACTGCCAACCGGGGAAACGGGCTGCGTGCTGTGGCTCCACTGCGCCCCGGGGAGCTGC TCTTCCGCTCCGACCCCTTGGCTTACACTGTGTGCAAGGGGAGTCGTGGCGTAGTCTGTGATCGCTGCCT ACTGGGGAAGGAGAAGCTGATGCGTTGTTCTCAATGCCGAATTGCCAAATACTGCAGTGCCAAGTGTCAG AAAAAAGCCTGGCCGGACCACAGACGGGAATGCAGTTGTCTTAAAAGCTGCAAGCCCAGATATCCGCCCG ACTCCGTGAGACTTCTGGGCAGGGTTATCGTCAAGCTGATGGATGAAAAGCCCTCAGAGTCAGAGAAACT TTATTCATTTTATGATCTGGAGTCCAATATTAGCAAACTCACTGAAGATAAGAAAGAGGGCCTGAGGCAA CTTGCAATGACTTTTCAACATTTCATGAGAGAGGAGATCCAGGATGCGTCTCAGCTGCCGCCTTCTTTTG ACCTTTTTGAAGCCTTTGCAAAAGTGATCTGCAATTCGTTCACTATCTGCAACGCGGAGATGCAGGAGGT CGGCGTTGGCCTGTACCCCAGTATGTCTTTGCTGAATCACAGCTGTGACCCCAACTGCTCCATCGTATTC AACGGGCCCCACCTCTTACTGCGTGCAGTGCGGGAAATTGAAGCAGGAGAGGAGCTCACCATCTGCTACC TGGACATGCTGATGACCAGCGAGGAACGCCGGAAGCAGCTGAGGGACCAGTACTGCTTTGAGTGTGACTG CATCCGATGCCAAACCCAGGACAAGGATGCTGACATGCTAACGGGTGACGAGCAAATATGGAAGGAGGTT CAAGAGTCGCTGAAGAAAATCGAAGAGCTGAAGGCGCACTGGAAGTGGGAGCAGGTTCTGGCGCTGTGCC AGGCGATCATAAACAGCAATTCCAACCGGCTTCCCGACATCAACATCTACCAGCTGAAGGTGCTCGACTG TGCCATGGATGCCTGCATCAACCTGGGGATGCTGGAGGAGGCGTTGTTCTACGCCATGCGCACCATGGAG CCGTACCGGATTTTTTTCCCTGGAAGCCATCCCGTCAGAGGTGTGCAAGTGATGAAAGTTGGCAAGCTGC AGCTCCATCAAGGCATGTTTCCTCAAGCCATGAAGAACCTGAGGCTGGCCTTTGACATTATGAAAGTGAC CCACGGCCGAGAGCACAGCCTGATTGAAGATTTGATTCTTCTCCTGGAAGAATGTGATGCCAACATACGA GCCTCCTAAGGACCCCCAGAAGGACGGGGAAGGTGGCGCGCCCTCCATCGAACGCCTTACTGAGGTCACA CGCTCTGTGGTGTTTGCTGTGTGGACCTTCTGTAGAAATTGCCAATGTGTTTATGTGGGTAAATGTGATT CTGTGGCATGTCGTGAGAAGTCTTACTCGTGATAGAGCAGAACCATTACAATAAATTAAAAAGACGAGTC CAAAATGCCACTGGCGACTTGGCTTTGCCTATTCTCAGGTGGTTTTGATGGTTAAGATGTCAACATTGGA AGCAGGAGCTTTCCAAAAGAAGAATGGAATCCCAGGCAGCCCCTGAAAGGCAGAGGCAGGTGGATCTCCA TGAGTTCGAGGCCAGTTTTGTCTATACAGTGAATTACAAGACAGCCATAGCCTTGTAGAGAGACCCTGTT CCAGAAACTGAAAAAAAATAGGGTAGAATATGTTCAAGTTTCCTCTGTGTGTGTGTGTATATAGAAGTAA ACGGATAAAAGTAATAATTTTCACATAGACTTCTAAACACAAGTGTATATCAAGAAGATTTTTTATTGTA CTTTTATCTTTATTGAGATGTCTTAATTGTACTTATTAACATGGTTCACTGTGATATTTCTATATCTGAA TACACACACATGGCGCCCAGTTGTCACTGAGGAACTCTGGCCTCCATTTATTTTCCTGCCTCCATGGATC ACCGTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACAC ACACACACACACACACCCTCCTGACTCTTACACATGAGAGGGAAAACGCAGTACTTGTCTCTGTCGTCCC CCCTTATTTCTCTTTGTATAATTTCCTCCAGTTCTGTCCATTCGGCTGCAGCTGACAGAGCTTCACTCTT CTTTATGGCTGAGTAATAGCCTGTTATTTGTATGTACTTGGTAAATGTTAGCAAGCACCTACAGTAACTG CAGCCTTGGCGATTATGAATGGCTTTATAGTAGACATGTCAGGCAAGTATGTTTGGGGTTTAATTTCCCT TTGACATACACCTAGAAGTGGCATGGCTGGGTCCCATGGTGGTACTCTTTTAACTTCTTAGATGAACCTC CTCCATACTCTCCTTGGCTTTAGGTTCCCATCAACAGTGTATGAGAGTTCCTGTTCTCCATGTTCACGTC AGCATTTGTTATGGGTTTTTTTTTCCTTGCTTTGCTGTTGGCTTTAATCGCATTTTAACTGGCGTACATG ATATTTCATCATAGTTTCGATTTCCCTGATGACCAATGGTATTGATTGTGCCTCATGTTTTTGTGACTGT TCAGAACAGTTGTTCATTTTGTAATCAGATTCTTGCCTTTTCAATTCCTTACCTATTATAAATACACTAT GTATTGTGGATATGACTCCTTCACTGGGAGGATGGTTGGCAGACAGTTCTCTAGTTCTGTAGATTGTCTT TTCCTCTGCTGTTTTGTGTGCTGTACAGAACCTCAGTGTAATTGTTTGTCTTCGGTTCTGATCCCCTCCC TTCTATAATCACATAAAGCCATTGCTTATTCTGGCTTCTATGATACTCCATTGCCCTTACTGACGTGTTC CCATGCATGTTCCCAGCTTGATCTTGCATTTGGTCTTTGGTCCATCGTGAGCTGAGATCCGTCCGTCGTG AAAAGGTTCGGTTTCCTTCTCTTGCATGTGGCTGTCTGCCTTCCCTGCACACTTTGTTGAAGAGGGAGTC ACACAGCCTTTATGGTTTGGGCACGTTTGTCAAGAAGGAGCGGGTTCTAGGTTTGTGGGCTTACTTTTGG GCTCTCCGTTCAGTCCCATGGGTCCCTTGCTCTATGGCCATGCCGTTTCTGCTAGCACGGCTCTGTAATA TGGCTTGCAGTCAGGTGCCATGTTGCCTCTGGCCTCACTGGTTTCACTCAAGATGATTTTGTCTGTCCTG AGTCTGTTGTGAAGAAGCAGAATTCTGAAACGTTGCCTTTACTCCTCTGCAGTGAATAACTACGTGTTAT TTCCTCCTGAATACCCCTCCAACACAGGAATCATCATCTCAAATCCTAGGTCTAGGCCATTTCCAACATT ACTAGGAAAACACTGTAAGGCTCACACAAAAAGAACAAGATACTGAAGTCAACTCTTTGTATAAATCTTT GCTGTACTTTATAATTCTTTGTGTGTGTTAAAACTTTCTTAACCCCCTTTCTTCTGTAATTGCTCTAGTC TGTGCTTTGCTGTGCTGGTGGGCAGAGCACAAAGCTCTATTGCCCTGTGGTAAACTGTGTTCTCATCCTC ATTCACCTTAGCCTGGTTTCCTCTGCTTTTGTTGTCTTTTCCACTTCCCCAACCCCTTGGCCTGCTTCTC TATCTTTCTGCTTTAGAAAAATAACAAAAATATTTCCCTTGTCCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6837012 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6837012 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11737 same embryo
	EMAGE:11733 same embryo
	EMAGE:11736 same embryo
	EMAGE:11735 same embryo
	EurExpress:euxassay_008432 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source