EMAGE mouse gene expression spatial database

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EMAGE:11745

Chrna7 cholinergic receptor, nicotinic, alpha polypeptide 7 ( MGI:99779)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008298. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008298_01	euxassay_008298_02	euxassay_008298_03	euxassay_008298_04

euxassay_008298_05	euxassay_008298_06	euxassay_008298_07	euxassay_008298_08	euxassay_008298_09

euxassay_008298_10	euxassay_008298_11	euxassay_008298_12	euxassay_008298_13	euxassay_008298_14

euxassay_008298_15	euxassay_008298_16	euxassay_008298_17	euxassay_008298_18	euxassay_008298_19

euxassay_008298_20	euxassay_008298_21	euxassay_008298_22	euxassay_008298_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11745_wholemount_strong.wlz

11745_wholemount_moderate.wlz

11745_wholemount_weak.wlz

11745_wholemount_possible.wlz

11745_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11745_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 07 09 10 11 13 14 15 16 17 moderate expression: see section 12 weak expression: see section 08

pons mantle layer

strong

single cell

strong expression: see section 06 07 09 13 16 17 18 weak expression: see section 08

trigeminal v nerve

strong

regional

strong expression: see section 05 06 07 08 10 11 16 17 18 moderate expression: see section 19

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 16

cervical ganglion

strong

regional

strong expression: see section 09 16 17

thoracic ganglion

moderate

regional

moderate expression: see section 13 14 15

tongue

strong

spotted

strong expression: see section 11 12 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35142
Entity Detected:	Chrna7, cholinergic receptor, nicotinic, alpha polypeptide 7 ( MGI:99779)
Sequence:	sense strand is shown >T35142 GCGCCGTAGGACACTCTACTATGGCCTCAACCTGCTCATTCCTTGTGTGCTCATTTCAGCCCTGGCTTTG CTGGTATTCTTGCTGCCTGCAGACTCTGGAGAGAAAATCTCTCTTGGAATAACTGTCTTACTTTCTCTGA CTGTCTTCATGCTGCTTGTGGCTGAGATCATGCCAGCAACATCTGATTCCGTGCCCTTGATAGCACAGTA CTTCGCCAGCACCATGATCATCGTGGGCCTCTCAGTGGTCGTGACAGTGATTGTGCTGCGATATCACCAC CATGACCCTGATGGTGGCAAAATGCCTAAGTGGACCAGGATCATTCTTCTGAATTGGTGTGCATGGTTTC TGCGCATGAAGAGGCCGGGAGAGGACAAGGTGCGGCCGGCTTGTCAGCACAAGCCTCGGCGCTGCAGCCT GGCCAGTGTGGAGCTGAGCGCAGGTGCGGGGCCACCCTCCAGCAATGGCAACCTGCTCTACATTGGCTTC CGAGGCCTGGAGGGCATGCACTGTGCCCCAACTCCAGACTCTGGGGTCGTGTGTGGTCGTTTGGCCTGCT CCCCAACACATGATGAGCACCTCATGCATGGTACACACCCCTCTGATG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95034. Forward Primer - name:095034_F_cDNA_Chrna7, sequence:GCGCCGTAGGACACTCTACTAT; Reverse Primer - name:095034_N_SP6_cDNA_Chrna7, sequence:CATCAGAGGGGTGTGTACCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11744 same embryo
	EMAGE:11746 same embryo
	EMAGE:11748 same embryo
	EMAGE:11747 same embryo
	EurExpress:euxassay_008298 same experiment
	MGI:4823860 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source