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EMAGE:11756

Kifc3 kinesin family member C3 ( MGI:109202)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756
"Pseudo-wholemount" of euxassay_010972. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010972_01 euxassay_010972_02 euxassay_010972_03 euxassay_010972_04
EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756
euxassay_010972_05 euxassay_010972_06 euxassay_010972_07 euxassay_010972_08 euxassay_010972_09
EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756
euxassay_010972_10 euxassay_010972_11 euxassay_010972_12 euxassay_010972_13 euxassay_010972_14
EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756
euxassay_010972_15 euxassay_010972_16 euxassay_010972_17 euxassay_010972_18 euxassay_010972_19
EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756 EMAGE:11756
euxassay_010972_20 euxassay_010972_21 euxassay_010972_22 euxassay_010972_23 euxassay_010972_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11756Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11756_wholemount_strong.wlz
11756_wholemount_moderate.wlz
11756_wholemount_weak.wlz
11756_wholemount_possible.wlz
11756_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11756_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pons mantle layer
weak weak
regionalweak expression: see section 09 10 15 16
left lung
strong strong
spottedstrong expression: see section 07 08 09 10 11 12
right lung
strong strong
spottedstrong expression: see section 14 15 16 18 moderate expression: see section 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36552
Entity Detected:Kifc3, kinesin family member C3 ( MGI:109202)
Sequence:sense strand is shown

>T36552
ACCTTTGACCCTGATGATGACTCCATCATCCACCTGCTGCACAAGGGCAAGCCCGTGTCCTTCGAGCTGG
ACAAGGTCTTCTCCCCGTGGGCTTCCCAGCAGGACGTGTTCCAGGAGGTGCAGGCCCTCATTACCTCCTG
CATCGATGGCTTCAATGTCTGTATCTTTGCTTACGGCCAGACAGGTGCCGGCAAGACATATACAATGGAG
GGGACTCCCGAGAACCCGGGCATCAACCAGCGGGCCCTGCAGCTGCTCTTCTCGGAGGTGCAGGAGAAGG
CGTCTGACTGGCAGTACAACATCACCGTCAGCGCACGTGAGATTTACAATGAAGTCCTCAGGGACCTGCT
GGGGAAAGAGCCTCAGGAGAAGCTGGAGATCCGACTGTGCCCGGATGGCAGTGGGCAACTGTATGTGCCA
GGGCTGACTGAGTTCCAGGTGCAGAGCGTGGATGACATCAACAAGGTGTTTGAGTTTGGCTACAACAACC
GCACCACCGAGTTCACCAACCTGAACGAACACAGCTCCCGCTCCCACGCTCTGCTCATCGTGACAGTGAG
AGGTGTGGATTGCAGAACAGGCCTCCGCACCACGGGGAAGCTGAACCTGGTGGACCTGGCTGGTTCAGAG
CGTGTGGGCAAGTCGGGGGCTGAGGGCAACCGCCTGCGAGAGGCACAGCACATCAACAGGTCACTATCCG
CCCTGGGGGACGTCATTGCTGCCCTGCGTTCTCGACAAGGCCATGTGCCCTTCCGCAATTCCAAACTCAC
CTACCTGCTGCAGGACTCACTCAGTGGTGACAGCAAGACCCTTATGGTGGTACAGGTGTCCCCAGTGGAG
AAGAACACCAGCGAGACTCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64125. Forward Primer - name:064125_F_cDNA_Kifc3, sequence:ACCTTTGACCCTGATGATGACT; Reverse Primer - name:064125_N_SP6_cDNA_Kifc3, sequence:CAGAGTCTCGCTGGTGTTCTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11757 same embryo
 EMAGE:11758 same embryo
 EMAGE:11755 same embryo
 EMAGE:11759 same embryo
 EurExpress:euxassay_010972 same experiment
 MGI:4825775 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS