EMAGE mouse gene expression spatial database

EMAGE:11760

Saa1 serum amyloid A 1 ( MGI:98221)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006979. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006979_01	euxassay_006979_02	euxassay_006979_03	euxassay_006979_04

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T10010
Entity Detected:	Saa1, serum amyloid A 1 ( MGI:98221)
Sequence:	sense strand is shown >T10010 ATAGATCACCAGATCTGCCCAGGAGACACCAGGATGAAGCTACTCACCAGCCTGGTCTTCTGCTCCCTGC TCCTGGGAGTCTGCCATGGAGGGTTTTTTTCATTTGTTCACGAGGCTTTCCAAGGGGCTGGGGACATGTG GCGAGCCTACACTGACATGAAGGAAGCTAACTGGAAAAACTCAGACAAATACTTCCATGCTCGGGGGAAC TATGATGCTGCTCAAAGGGGTCCCGGGGGAGTCTGGGCTGCTGAGAAAATCAGTGATGGAAGAGAGGCCT TTCAGGAATTCTTCGGCAGAGGACATGAGGACACCATTGCTGACCAGGAAGCCAACAGACATGGCCGCAG TGGCAAAGACCCCAATTACTACAGACCTCCTGGACTGCCTGACAAATACTGAGCGTCCTCCTATTAGCTC AGTAGGTTGTGCTGGGGGCCTGAGGGTGGGGTCTGGGCTTCTTCCTACCTAGGAACACTGAAGATGCTCT CTGGGGAAACATTGTATATCTCTCATGTGTGTATCCCACAAGGGTTTCAGAACTGAGTTACTCTTGCAGT AGTAACTGCTTGAGGAGGAGAGGGTAATAAACAGAAACTTGGAAGCGAAAAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30310171 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30310171 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006979 same experiment
	EMAGE:31835 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source