EMAGE mouse gene expression spatial database

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EMAGE:11781

Irs4 insulin receptor substrate 4 ( MGI:1338009)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011002. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011002_01	euxassay_011002_02	euxassay_011002_03	euxassay_011002_04

euxassay_011002_05	euxassay_011002_06	euxassay_011002_07	euxassay_011002_08	euxassay_011002_09

euxassay_011002_10	euxassay_011002_11	euxassay_011002_12	euxassay_011002_13	euxassay_011002_14

euxassay_011002_15	euxassay_011002_16	euxassay_011002_17	euxassay_011002_18	euxassay_011002_19

euxassay_011002_20	euxassay_011002_21	euxassay_011002_22	euxassay_011002_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11781_wholemount_strong.wlz

11781_wholemount_moderate.wlz

11781_wholemount_weak.wlz

11781_wholemount_possible.wlz

11781_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11781_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

weak

regional

weak expression: see section 05 06 19 20 21

thyroid gland

strong

regional

strong expression: see section 11 14 15

pituitary gland

strong

regional

strong expression: see section 10 11 12 13 14 15 16

hypothalamus mantle layer

moderate

spotted

moderate expression: see section 10 11 14 15 weak expression: see section 09

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 11 12 14 15

midbrain mantle layer

moderate

spotted

moderate expression: see section 07 08 10 14 15 16 17 weak expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36522
Entity Detected:	Irs4, insulin receptor substrate 4 ( MGI:1338009)
Sequence:	sense strand is shown >T36522 AGAGCTTTCGATGAAGATGAGGATGACCCGTATGTGCCAATGAGACCAGGGGTGGCTGCCCCTCTTGCGT GTTCCAGTGATTATATGCCAATGGCTCCTCAAAATTCCTCTGCTTCAACAAAACGCCACTCTAGGTCACC CTTTGAAGACTCCAGAGGGTACATGATGATGTTTCCCAGAGTGAGCCCCCCACCACCTGTGCCAAGTGCT CCGAAAGCACCGGACACCAATAAAGGGGATGACTCAAAGGACAATGACAGTGACAGTGATTACATGTTTA TGGCTCCCGGAGCAGGTGCGATTCCTAAAAATCCTCCCAATGCGCAGGGAGGCTCTTCCTCCAAAAGCTG GAGCTCGTATTTCTCTCTGCCAAGTCCTTTTCAGAGTTCACCTTTGGGACAGAGTGACCACAGTGAGTAT GTTCCTATGTTACCTGGAAAATTCCTGGGGAGCGGTCTACACAAAGAAGCCTCCTTTAGCCAGGGCACCA AAAATGTGTCTTCAAAGCCTTCAACTGAGGCGTCATTCTCAAAACCCGAAGATAAAGGGTCATCTGCAAA GCCTTCAGATGATGTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99169. Forward Primer - name:099169_F_cDNA_Irs4, sequence:AGAGCTTTCGATGAAGATGAGG; Reverse Primer - name:099169_N_SP6_cDNA_Irs4, sequence:GCACATCATCTGAAGGCTTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11782 same embryo
	EMAGE:11785 same embryo
	EMAGE:11784 same embryo
	EMAGE:11783 same embryo
	EurExpress:euxassay_011002 same experiment
	MGI:4825621 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source