EMAGE mouse gene expression spatial database

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EMAGE:11799

Arhgef2 rho/rac guanine nucleotide exchange factor (GEF) 2 ( MGI:103264)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007105_01	euxassay_007105_02	euxassay_007105_03	euxassay_007105_04	euxassay_007105_05

euxassay_007105_06	euxassay_007105_07	euxassay_007105_08	euxassay_007105_09	euxassay_007105_10

euxassay_007105_11	euxassay_007105_12	euxassay_007105_13	euxassay_007105_14	euxassay_007105_15

euxassay_007105_16	euxassay_007105_17	euxassay_007105_18	euxassay_007105_19	euxassay_007105_20

euxassay_007105_21	euxassay_007105_22	euxassay_007105_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11799_wholemount_strong.wlz

11799_wholemount_moderate.wlz

11799_wholemount_weak.wlz

11799_wholemount_possible.wlz

11799_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11799_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6474
Entity Detected:	Arhgef2, rho/rac guanine nucleotide exchange factor (GEF) 2 ( MGI:103264)
Sequence:	sense strand is shown >T6474 GTGCGGCCGGACCCCTCCCCCGCCGTTGCTGGACACCCGGGGCCGCCGCGTCCTCCCGCGGCTCACGCTG GATTATGTCTCGGATCGAATCCCTCACTCGCGCGCGGATCGACCGGAGCAAGGAGCAGGCAACCAAGACC CGGGAAAAGGAGAAGATGAAGGAAGCTAAAGATGCCCGCTATACCAACGGCCACCTCTTCACCACCATCT CCGTCTCCGGCATGACCATGTGCTATGCCTGTAACAAGAGCATCACAGCCAAGGAAGCCCTCATTTGTCC TACATGTAACGTGACCATCCACAACCGCTGTAAAGACACCCTGGCCAACTGTACCAAGGTCAAGCAGAAG CAACAGAAAGCTGCACTGCTGAGGAACAACACTGCTTTGCAGTCTGTCTCCCTTCGAAGTAAGACGACCA CCAGAGAGCGGCCAACGTCTGCCATTTACCCTTCCGACAGCTTCCGGCAGTCCCTCCTGGGTTCTCGGCG GGGCCTCTCCTCCTTATCTTTGGCCAAAAGTGTTTCCACTACCAACATTGCTGGACATTTCAATGATGAG TCTCCTCTGGGGCTGCGTCAGATCCTCTCCCAGTCCACAGACTCCCTCAACATGCGGAACCGAACCCTGT CCGTGGAATCCCTTATTGATGAAGGTGTAGAAGTGTTCTACAATGAGCTGATGAGCGACTTTGAGATGGA TGAGAAGGACTTTGAGGCGGATTCTTGGAGCCTTGCCGTGGACAGCAGCTTCCTGCAGCAGCACAAAAAG GAAGTGATGAAGAAGCAAGATGTCATCTATGAGCTGATCCAGACAGAGCTGCACCACGTGAGAACCTTGA AGATTATGACCCGCCTCTTTCGCACTGGGATGCTGGAAGAGTTGCAGATGGAGCCAGAAGTGGTCCAGGG CCTGTTCCCCTGCGTGGATGAACTCAGTGACATTCACACACGTTTCCTTAATCAGCTTCTGGAACGGCGG CGCCAGGCTCTATGTCCAGGCAGCACCCGGAACTTTGTCATCCATCGTTTGGGTGACTTGCTCATCAGTC AGTTCTCAGGTTCCAATGCTGAGCAGATGCGCAAGACCTACTCAGAGTTCTGCAGCCGCCACACCAAGGC CTTAAAGCTCTATAAGGAGCTGTATGCTCGAGACAAACGCTTCCAACAGTTCATCCGGAAAATGACCCGC TCAGCTGTGTTGAAGCGGCATGGAGTTCAGGAATGCATTCTCCTGGTGACTCAGCGGATCACCAAATACC CTGTGCTCATCAACCGAATCCTGCAGAATTCCCACGGGGTTGAAGAAGAGTACCAAGACTTGGCGTCAGC CCTAGGACTAGTGAAGGAGTTGTTGTCCAATGTGGACCAGGATGTGCACGAGCTGGAGAAAGAGGCCCGC CTGCAGGAGATTTACAACCGAATGGATCCCCGGGCTCAGACCCCCGTACCTGGCAAGGGCCCCTTCGGCC GAGATGAACTTTTACGGAGAAAACTTATCCATGAAGGCTGCCTGCTCTGGAAGACAGCCACAGGCCGCTT CAAAGATGTCCTGTTGCTGCTGATGACAGACGTGCTCGTGTTTCTCCAGGAAAAGGACCAGAAATACATT TTCACATCCCTGGACAAGCCCTCAGTGGTGTCCTTGCAGAACCTCATCGTAAGAGACATAGCCAACCAGG CGAAAGGGATGTTTCTGATTAGTTCTGGACCGCCTGAGATGTATGAGGTGCATGCGGCGTCCCGAGACGA CCGGACTACCTGGATCCGTGTCATCCAGCAGAGTGTGCGCCTGTGCCCGTCCAGGGAGGACTTTCCTCTG ATCGAGACAGAGGATAAGGCGTATCTCCGGAGGATCAAGACGAAACTGCAGCAGAAAAACCAGGCGCTAG TGGAGCTGCTACAGAAGAATGTTGAGCTGTTTGCCGAGATGGTCCACTTCCAGGCCTTAAAGGCTGGCTT CGTTGGAATGCCCCCACCCGCCCTGCCCAGGGGCCTTTTCCGTCTTGAGTCCTTTGAGTCCCTCCGAGGC GAGCGCCTGCTAAAGGATGCCCTCCGTGAAGTGGAAGGCCTGAAAGACCTGCTGTTGGGCCCATGTGTGG ACCTGCCAACGACATCCCGAGAACCAGCCTTACCCTTGGACTCTGACAGCGGTAGCTGTCCTGGGGTCAC TGCCAATGGAGAGGCCAGAACCTTCAATGGCTCCATTGAACTCTGTAGAGCAGACTCAGATTCCAGCCAG AAGGATCGGAATGGAAATCAGTTGAGGTCACCACAGGAGGAGGTGTTACAGCCATTGATCAATCTTTATG GACTTCTACATGGCCTGCAGGCTGTTGTGGTCCAGCAGGAAAGACTGATGGAAGCCCTGTTCCCTGAGGG CCCTGAACGGTGGGAAAAGCTATCCCGAGCCAACTCTCGGGATGGTGAAGCTGGCCGGGCTGCGGTTGCT TCTGTAACTCCTGAGAAGCAGGCCACGGAGCTGGCACTACTGCAGAGGCAACACACCCTGTTGCAGGAAG AGCTGCGGCGCTGCCAGCGGCTCGGGGAAGAGCGGGCAACTGAAGCTGGCAGCCTGGAAGCCAGGCTCCG AGAGAGCGAGCAAGCCCGGGCCCTGCTGGAGCGGGAGGCTGAAGAGATCCGCCGGCAGCTTGCAGCCTTG GGCCAAAACGAGCCACTCCCGGCAGAAGCGCCCTGGGCTCGCAGGCCTCTGGACCCACGGCGCCGCAGCC TTCCAGCGGGCGACGCTTTATACTTGAGCTTCAATCCCCCCCAGCCCAGTCGAGGCCATGACCGCCTGGA TTTGCCTGTGACTGTTCGTTCCCTCCACCGACCCTTTGATGACCGAGAGGCGCAAGAACTTGGTAGCCCC GAGGATCGACTGCAGGACAGCAGTGACCCTGATACTGGTAGTGAGGAGGAAGTCAGTAGCCGCCTGTCTC CACCTCACAGTCCTCGAGACTTCACCCGAATGCAGGACATTCCTGAAGAGACAGAAAGCCGAGATGGGGA GCCCACAGCTTCAGAGAGCTAAGGGGACACTTCCCCCACCCCCGCCTGGTGCCCCACTGAAGAACATTAG TAAAGGGGGCAAACCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3587800 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3587800 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11803 same embryo
	EMAGE:11801 same embryo
	EMAGE:11802 same embryo
	EMAGE:11804 same embryo
	EMAGE:11800 same embryo
	EurExpress:euxassay_007105 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source