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EMAGE:11800

Serpini1 serine (or cysteine) peptidase inhibitor, clade I, member 1 ( MGI:1194506)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800
"Pseudo-wholemount" of euxassay_007129. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007129_01 euxassay_007129_02 euxassay_007129_03 euxassay_007129_04
EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800
euxassay_007129_05 euxassay_007129_06 euxassay_007129_07 euxassay_007129_08 euxassay_007129_09
EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800
euxassay_007129_10 euxassay_007129_11 euxassay_007129_12 euxassay_007129_13 euxassay_007129_14
EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800
euxassay_007129_15 euxassay_007129_16 euxassay_007129_17 euxassay_007129_18 euxassay_007129_19
EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800 EMAGE:11800
euxassay_007129_20 euxassay_007129_21 euxassay_007129_22 euxassay_007129_23 euxassay_007129_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11800Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11800_wholemount_strong.wlz
11800_wholemount_moderate.wlz
11800_wholemount_weak.wlz
11800_wholemount_possible.wlz
11800_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11800_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6495
Entity Detected:Serpini1, serine (or cysteine) peptidase inhibitor, clade I, member 1 ( MGI:1194506)
Sequence:sense strand is shown

>T6495
GCAGCATCCGGAGCAGTCTCAGCCTGCCCAGCATCCTCTCCAGCATCCCGAGCGGGGATTGCAGGTGTGA
AGGAGACTTGAAACCATCCCATCATGACTTACCTTGGACTGCTTGCTTTGCTGGCCTTGCAAAGTGTGGT
GACAGGGGCAACGTTCCCAGATGAAACCATAACTGAGTGGTCAGTGAACATGTATAACCACCTTCGAGGC
ACCGGGGAAGATGAAAACATTCTCTTCTCTCCACTAAGCATTGCCCTTGCGATGGGAATGATGGAGCTTG
GGGCTCAAGGATCTACTAGGAAAGAAATCCGCCATTCAATGGGATATGAGGGTCTGAAAGGTGGTGAAGA
ATTTTCTTTCCTGAGGGATTTTTCTAATATGGCCTCTGCCGAAGAAAACCAATATGTGATGAAACTTGCC
AATTCGCTCTTTGTACAAAATGGATTTCATGTCAATGAGGAATTCTTGCAAATGTTGAAAATGTACTTTA
ATGCAGAAGTCAATCATGTGGACTTCAGTCAAAATGTGGCTGTGGCTAACTCCATCAATAAATGGGTGGA
GAATTATACAAACAGTCTGTTGAAAGATCTGGTGTCTCCGGAGGACTTTGATGGTGTCACTAATTTGGCC
CTCATCAATGCTGTATATTTCAAAGGAAACTGGAAGTCTCAGTTTAGACCTGAAAATACCAGAACTTTCT
CCTTCACGAAAGATGATGAAAGTGAAGTGCAGATTCCAATGATGTATCAACAAGGAGAATTTTATTATGG
TGAATTTAGTGATGGATCCAATGAGGCTGGCGGTATCTACCAAGTCCTTGAGATACCCTATGAGGGAGAT
GAGATCAGCATGATGCTGGCACTGTCCAGACAGGAAGTCCCACTGGCCACACTGGAGCCTCTGCTCAAAG
CACAGCTGATCGAAGAATGGGCAAACTCTGTGAAGAAACAAAAGGTGGAAGTGTACTTGCCCAGGTTCAC
TGTGGAACAGGAAATTGATTTAAAAGACATCTTGAAAGCCCTTGGGGTCACTGAAATTTTCATCAAAGAT
GCAAATTTGACTGCCATGTCAGATAAGAAAGAGCTGTTCCTCTCCAAAGCTGTTCACAAGTCCTGCATTG
AGGTTAATGAAGAAGGGTCAGAAGCCGCTGCAGCCTCCGGAATGATTGCGATTAGTAGGATGGCTGTGCT
GTACCCTCAGGTTATTGTCGACCATCCATTTCTCTATCTTATCAGGAACAGGAAATCTGGCATAATCTTA
TTCATGGGACGAGTCATGAACCCTGAAACAATGAATACAAGTGGCCATGACTTTGAGGAACTTTAAATGA
CGACGTTTGAGTACAAAGAAAGCAGGAACAAAGCACATTATGTTTGCAAGTGGTATATATTTAGGATTTC
TGTTTTATAGTGTTATTTAGGGAAATATTTAAATAGTTCTAGATAGTAGTAATCCATGTGACCTATAAGT
TAGCCTGTCAAAAGCTGTTATCAGTATAAAGAGTATGGTCCCATTGTGTCATTGTGTCTGGTGTGCTGCT
GTTTAAAATAAAAGTACATATTGAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3588856 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3588856 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11803 same embryo
 EMAGE:11801 same embryo
 EMAGE:11802 same embryo
 EMAGE:11804 same embryo
 EMAGE:11799 same embryo
 EurExpress:euxassay_007129 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS