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EMAGE:11801

Clca2 chloride channel calcium activated 2 ( MGI:1931471)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801
"Pseudo-wholemount" of euxassay_007074. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007074_01 euxassay_007074_02 euxassay_007074_03 euxassay_007074_04
EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801
euxassay_007074_05 euxassay_007074_06 euxassay_007074_07 euxassay_007074_08 euxassay_007074_09
EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801
euxassay_007074_10 euxassay_007074_11 euxassay_007074_12 euxassay_007074_13 euxassay_007074_14
EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801
euxassay_007074_15 euxassay_007074_16 euxassay_007074_17 euxassay_007074_18 euxassay_007074_19
EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801 EMAGE:11801
euxassay_007074_20 euxassay_007074_21 euxassay_007074_22 euxassay_007074_23 euxassay_007074_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11801Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11801_wholemount_strong.wlz
11801_wholemount_moderate.wlz
11801_wholemount_weak.wlz
11801_wholemount_possible.wlz
11801_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11801_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
adrenal gland
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 18 19 20 21
thymus primordium
moderate moderate
regionalmoderate expression: see section 12 13 15
pancreas
strong strong
regionalstrong expression: see section 10 11 moderate expression: see section 12
thyroid gland
strong strong
regionalstrong expression: see section 11 16
hypothalamus mantle layer
moderate moderate
regionalmoderate expression: see section 10 15 weak expression: see section 08 09 16 17
thalamus mantle layer
weak weak
regionalweak expression: see section 07 08 17 18
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 09 10 15 16 17
pons mantle layer
moderate moderate
regionalmoderate expression: see section 07 17 18
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
aorta
strong strong
regionalstrong expression: see section 11 12 13 14 15
heart atrium
moderate moderate
regionalmoderate expression: see section 07 08 09 10 14 15 16 17 18 19 20 21 22 23
heart ventricle
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17
midgut
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14 15 16 17 18
liver lobe
strong strong
single cellstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
bladder
strong strong
regionalstrong expression: see section 12 13 14 15
metanephros
strong strong
regionalstrong expression: see section 09 10 11 18 19 20 21
ovary
strong strong
regionalstrong expression: see section 05 06 07 08 09 21 22 23
urethra of male
strong strong
regionalstrong expression: see section 13 14 15
trachea
weak weak
regionalweak expression: see section 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6411
Entity Detected:Clca2, chloride channel calcium activated 2 ( MGI:1931471)
Sequence:sense strand is shown

>T6411
CGGACGCGTGGGTGCGACCATGGTGCCAGGGCTGCAGGTCCTTCTGTTCCTCACCCTGCATCTCCTGCAG
AACACAGAGAGCTCCATGGTGCATCTCAACAGCAATGGATACGAGGGTGTGGTCATTGCCATTAACCCCA
GTGTGCCAGAGGACGAAAGGCTCATCCCAAGCATAAAGGAAATGGTAACTCAAGCTTCTACCTACCTGTT
TGAAGCCAGCCAAGGAAGAGTTTATTTCAGGAACATAAGCATATTAGTCCCGATGACCTGGAAGTCAAAA
TCTGAGTACTTAATGCCAAAACGAGAATCGTACGACAAAGCAGACGTCATAGTTGCGGATCCTCACCTGC
AACATGGAGACGACCCCTACACCCTTCAGTATGGACAGTGTGGGGACAGAGGACAGTACATACACTTCAC
TCCAAACTTCCTACTCACTGATAACTTGCGTATCTATGGACCCCGAGGCAGAGTCTTTGTCCATGAGTGG
GCCCATCTCCGGTGGGGAGTATTTGATGAGTATAACGTGGACCGGCCTTTCTACATTTCTAGAAAGAACA
CTATAGAAGCAACAAGGTGCTCCGCCAGCATCACAGGCAAGAAGGTGGTCCACGAGTGTCAGAGAGGCAG
CTGTGTGACAAGGGCGTGCCGGCGTGACTCGAAGACACGGCTGTATGAACCCAAATGTACATTTATCCCA
GACAAAATACAGACAGCTGGGGCCTCCATAATGTTCATGCAAAACCTCAATTCTGTGGTTGAATTTTGCA
CAGAAAATAACCACAATGCAGAAGCCCCAAACCTACAAAACAAAATGTGCAATCGCAGAAGCACGTGGGA
TGTAATCAAGGCGTCTGCTGACTTTCAGAATTCCCCTCCCATGAGAGGAACAGAAGCGCCTCCTCCACCT
ACATTTTCATTGCTCAAGTCCAGAAGGCGAGTGGTGTGCTTGGTGCTGGATAAATCTGGAAGCATGGACA
AAGAAGACCGTCTTATTCGAATGAATCAAGCAGCAGAACTGTACTTAACTCAAATTGTGGAAAAGGAGTC
TATGGTTGGATTAGTCACATTTGACAGCGCTGCCCACATCCAAAATTATCTAATAAAAATAACGAGTAGT
AGTGACTACCAAAAGATCACCGCAAACCTCCCCCAACAGGCTACTGGTGGAACTTCAATTTGCCATGGAC
TCCAGGCAGGATTTCAGGCAATTACCTCCAGTGACCAGAGCACTTCCGGTTCTGAGATCGTATTGCTGAC
AGATGGGGAAGATAATGGAATAAGTTCCTGCTTTGAGGCCGTCTCTCGCAGCGGTGCCATCATCCACACC
ATCGCTCTGGGGCCTTCTGCTGCCCGAGAACTGGAGACTCTGTCAGACATGACAGGAGGGCTTCGTTTCT
ATGCCAACAAACATGTAAGCAGCCTTATCGACGCTTTCAGTAGGATTTCATCTACAAGTGGCAGCGTCTC
CCAGCAGGCTCTGCAGTTGGAGAGCAAAGCCTTCAATGTCAGAGCAGGGGCATGGATAAACAGTACAGTA
CCTGTGGACAGTACCGTCGGCAACGACACGTTCTTTGTTATCACCTGGACGGTACAAAAGCCAGAAATCA
TTCTTCAAGATCCAAAAGGAAAAAAATATATAACCTCAGATTTCCAAGATGATGAACTAAACATCCGGTC
TGCTCGACTTCAAATACCGGGCACTGCAGAGACAGGTACTTGGACTTACAGCATCACGGGTACCAAGTCT
CAGTTGATTACAATGACAGTGACCACTCGAGCAAGAAGTCCCACCATGGAACCACTCCTGGCTACCGCTC
ACATGAGTCAGAGCACAGCCCAGTACCCTAGCCGGATGATTGTGTATGTACGGGTCAGCCAAGGGTTTTT
GCCTGTTCTAGGAGCCAATGTCACAGCCATCATAGAAGCTGAACATGGACATCAAGTCACCCTGGAGCTA
TGGGACAATGGGGCAGGTGCTGATACTGTTAAAAATGATGGCATCTACACAAGATACTTTACAGATTATC
ATGGAAATGGTAGATACAGCCTAAAAGTGCGTGTCCAGGCAAGAAAAAACAAAGCCAGACTAAGCTTAAG
ACAGAAGAACAAGTCTTTATATATACCTGGCTATGTGGAAAATGGTAAAATTGTACTGAATCCACCCAGA
CCAGATGTCCAAGAAGAAGCCATAGAAGCTACAGTGGAGGACTTCAACAGAGTAACCTCTGGAGGGTCAT
TTACTGTGTCTGGAGCGCCCCCTGATGGCGACCATGCTCGTGTGTTCCCACCAAGTAAAGTCACAGACCT
GGAGGCTGAGTTTATAGGTGATTATATTCAACTTACATGGACGGCCCCTGGCAAGGTTCTCGATAAGGGA
AGAGCGCATAGATACATCATCAGAGTGAGCCAGCATCCTCTGGGTCTCCAAGAAGATTTTAACAATGCTA
CTTTAGTGAATGCTTCCAGTCTGATACCTAAAGAGGCTGGCTCAAAAGAAACATTTAAATTCAAACCAGA
AACTTTTAAAATAGCAAATGACACCCAGCTCTACATTGCAATCCAGGCATACAATGAAGCTGGTCTCACC
TCTGAGGTCTCCAACATTGCACAGGCTGTCAAGTTTACTTCTCTAGAAGATAGTATCTCTGCACTGGGTG
CTGATATTTCTGCAATCTCTATGACAGTTTGGGGGTTAGCTGTGATTTTTAACTCTATTTTAAACTAGAA
GATACAATAGCACTAAAATGCAACCCTGTATATATTTGCTGACTGTTGCTTTAGAATGACTTTACTACAC
TCAAAGGCTGCCTGTCAACACTTGTAATATAGAAGTTCATATTCAAAGTTGAAAATCCCGAGTTACTAAC
AACATTCTTCTGCTATATGTAGATCAAGATTAACAGTTCCTCATTCAATTTCCTAATTGTTCCATTTACT
ATGGAAATTAGATATCCATTCCCTTTTCACAGTGTGATGCAGGTTCACTTTACAAAATATTACCAAAATA
AAATTTGTATAAGTCAATTAAAGGGAATTATTCTTATATAATAGCAAACAATTTTATAGTAGAATTATTT
GTAAGACAATTAATAAAGAATGCTACCTGCTTTTACAACTGAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3583804 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3583804 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11803 same embryo
 EMAGE:11802 same embryo
 EMAGE:11804 same embryo
 EMAGE:11799 same embryo
 EMAGE:11800 same embryo
 EurExpress:euxassay_007074 same experiment
 MGI:4823893 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS