EMAGE mouse gene expression spatial database

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EMAGE:11802

Rnf141 ring finger protein 141 ( MGI:1914400)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007088. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007088_01	euxassay_007088_02	euxassay_007088_03	euxassay_007088_04

euxassay_007088_05	euxassay_007088_06	euxassay_007088_07	euxassay_007088_08	euxassay_007088_09

euxassay_007088_10	euxassay_007088_11	euxassay_007088_12	euxassay_007088_13	euxassay_007088_14

euxassay_007088_15	euxassay_007088_16	euxassay_007088_17	euxassay_007088_18	euxassay_007088_19

euxassay_007088_20	euxassay_007088_21	euxassay_007088_22	euxassay_007088_23	euxassay_007088_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11802_wholemount_strong.wlz

11802_wholemount_moderate.wlz

11802_wholemount_weak.wlz

11802_wholemount_possible.wlz

11802_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11802_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6399
Entity Detected:	Rnf141, ring finger protein 141 ( MGI:1914400)
Sequence:	sense strand is shown >T6399 GTGTCCCTGGTGCGAGGAGAGCGATGCCTTGAACTGGAAACTATGGGAAGCAAAACTCTTTCTGAATTCT GAAAGACATCATTTCACCATGGGACAGCAAATTTCGGATCAGACGCAGTTGGTTATTAACAAGTTACCAG AAAAGGTAGCAAAGCACGTCACCTTGGTTCGAGAAAGTGGCTCCTTAACTTACGAAGAGTTTCTTGGGAG AGTAGCTGAACTCAATGATGTAACTGCTAAGGTGGCTGCTGGCCAGGAAAAGCATCTTCTCTTTGAGGTA CAACCTGGGTCTGACTCCTCTGCCTTCTGGAAAGTAGTTGTACGGGTGGTCTGTACCAAGATTAACAAAA GCAGTGGCATTGTGGAAGCATCGCGGATCATGAATTTGTACCAGTTTATTCAGCTCTATAAAGACATCAC AAGCCAAGCTGCTGGAGTGCTGGCTCAGAGCTCCACCTCTGAAGAGCCTGATGAGAACCCATCCTCGGTC ACATCTTGCCAGGCTAGTCTATGGATGGGCAGGGTGAAGCAGCTGACTGATGAGGAGGAGTGTTGTATCT GCATGGACGGGCGAGCTGACCTCATCCTGCCCTGTGCTCACAGCTTCTGTCAGAAGTGCATTGATAAATG GAGTGATCGACACAGGAACTGCCCCATTTGTCGCCTACAGATGACTGGAGCAAATGAATCATGGGTGGTG TCAGATGCACCCACTGAAGATGACATGGCTAACTATATTCTTAACATGGCTGATGAGGCAGGCCAGCCCC ACAGGCCATGACTTGGGGAAGACTTTCTGTTGTTATTCTGAACTACAAATACACTGTTGTCGTAGAGGAG AAGGGGGGGATATTGTGGCACACACAGAAATATCAGTTTTCCCACCCTCTTATTTTTGCTATTCTGGTAT GTCCCGTTCTTAAATAAACTCCCATGTCTTCCATGTATGGTGGGCTACAGTTTGCTACTGTTTAGCCATA TTTCCCACAGTCCACCTATTCTATGTTGCTCTTGAGCCCTTTGCTACAGATAGCAGCAATACTTGACACT ACTGCTTTTCAGGGAGGGAGGGACTGTTCTGGCATCTTTTTAGTAGGTTTAAGTTTTAGAGTGTTGACAG TTGTAGCCATCTGAGATAAATGACTGAAACTAATGTCTCAGTTGGAACCTGAGGTGTGTGTGCTCTGCCC CCTTGTGGTTCATTATGGATCCTTCAGTACTGTGGCCCAATTGCTTCCTGATGTTATGTCACTTTTGGCC TTGGGTTCTGGAACTACTTATTCTTTATGTTGCCATGATGCAGCACACGGATTAGGACTGTCTGGTTGGA ACTTTGTCTCGAAAATGGACTGTACCGGAGGGATCTTCATGGCTGCTTTAGAAAGGCATCCCCGGACCTG GATAGGTGCCCGTTGGATTTTTTCATAATGCCCATGAAGCTTGAGTTTTCAGAAGGATTTTTTTCTTTTA TAGAAACACACATTTTATATTTCCAGATTCCACAGAGGCATCCAGTCCTGAGCCAGACAGCGTTTATTTT AGGTAAAGACGCCTTTCTGAGGCTTCGGTTTCCTCAAATTTCTCAGATTAAAAGAAGAGGACGATCAGGT AAACCTCCTCACCAAGCAGTTGTAAAACCCTAAACTCCAGACCTTGATTGGCTTTCTTCCCATTTCTCTG CACTATTCAGGCATCTAGAGAGAGAACCGTGACAGGAGTGCGGCCTCAGTGACAGCCACAGTGGCTTATG GAGGAAGAACGTTAATGCTAACAGTGCAGCCTATAAAAGTGCTAGTGCATGAAATAAGTCCCCTGTAAGA AATGTTAGCGTTCCATAGAAGTCTTCTCACAGATTTTTGGCAAGTTTCAACTGTCCCTGTTTTCCTAAGT ATAGATGTACACAATTGAAGTTACTGCTTATTGATTTTTGATTCAGATTTTAATGAATATTTAAATAAAA TCCCTTTCAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3582546 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3582546 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11803 same embryo
	EMAGE:11801 same embryo
	EMAGE:11804 same embryo
	EMAGE:11799 same embryo
	EMAGE:11800 same embryo
	EurExpress:euxassay_007088 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source