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EMAGE:11811

Cckbr cholecystokinin B receptor ( MGI:99479)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11811 EMAGE:11811 EMAGE:11811 EMAGE:11811 EMAGE:11811
"Pseudo-wholemount" of euxassay_007150. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007150_01 euxassay_007150_02 euxassay_007150_03 euxassay_007150_04
EMAGE:11811 EMAGE:11811 EMAGE:11811 EMAGE:11811 EMAGE:11811
euxassay_007150_05 euxassay_007150_06 euxassay_007150_07 euxassay_007150_08 euxassay_007150_09
EMAGE:11811 EMAGE:11811 EMAGE:11811 EMAGE:11811 EMAGE:11811
euxassay_007150_10 euxassay_007150_11 euxassay_007150_12 euxassay_007150_13 euxassay_007150_14
EMAGE:11811 EMAGE:11811 EMAGE:11811 EMAGE:11811 EMAGE:11811
euxassay_007150_15 euxassay_007150_16 euxassay_007150_17 euxassay_007150_18 euxassay_007150_19
EMAGE:11811 EMAGE:11811 EMAGE:11811 EMAGE:11811
euxassay_007150_20 euxassay_007150_21 euxassay_007150_22 euxassay_007150_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11811Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11811_wholemount_strong.wlz
11811_wholemount_moderate.wlz
11811_wholemount_weak.wlz
11811_wholemount_possible.wlz
11811_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11811_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35125
Entity Detected:Cckbr, cholecystokinin B receptor ( MGI:99479)
Sequence:sense strand is shown

>T35125
GATGGTGATAATGACAGCGAGACCCAAAGCCGGGTCCGAAACCAAGGGGGTCTGCCGGGTGGGGCAGCAG
CACCAGGGCCTGTCCACCAGAACGGGGGTTGCCGGCATGTGACCAGCCTGACTGGGGAAGACAGTGATGG
CTGCTACGTGCAACTTCCGCGTTCCCGACTGGAGATGACAACGCTGACCACGCCCACTACTGGGCCAGGC
CCTGGCCCTCGGCCCAACCAGGCCAAGCTGCTGGCTAAGAAGCGGGTGGTGCGAATGCTGCTGGTGATTG
TTTTGCTTTTTTTCGTGTGTTGGCTGCCAGTGTACAGCGCCAACACGTGGCGTGCCTTCGATGGCCCAGG
CGCGCGCCGAGCACTCGCAGGGGCCCCTATCTCTTTCATCCACTTGCTGAGCTACACCTCTGCTTGTGCC
AACCCCCTGGTCTACTGTTTCATGCACCGCCGATTCCGCCAGGCCTGCCTGGACACATGTGCCCGCTGTT
GCCCACGCCCTCCACGAGCTCGCCCACGGCCTCTTCCGGATGAGGATCCTCCTACCCCCTCCATCGCTTC
TCTGTCCAGGCTAAGCTATACCACCATCAGCACGCTGGGACCTGGCTGAGGGATTGGGAGAGTGGAGAGT
GAGATGAGATACATAACTACTATAAAATGACCCATCCAAACACATAACAAAATCCGGGATTAATTCAGGT
GGTACCCAACATCATGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94214. Forward Primer - name:094214_F_cDNA_Cckbr, sequence:GATGGTGATAATGACAGCGAGA; Reverse Primer - name:094214_N_SP6_cDNA_Cckbr, sequence:CCATGATGTTGGGTACCACCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11812 same embryo
 EMAGE:11815 same embryo
 EMAGE:11814 same embryo
 EMAGE:11813 same embryo
 EurExpress:euxassay_007150 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS