EMAGE mouse gene expression spatial database

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EMAGE:11829

Plk3 polo-like kinase 3 (Drosophila) ( MGI:109604)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007086. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007086_01	euxassay_007086_02	euxassay_007086_03	euxassay_007086_04

euxassay_007086_05	euxassay_007086_06	euxassay_007086_07	euxassay_007086_08	euxassay_007086_09

euxassay_007086_10	euxassay_007086_11	euxassay_007086_12	euxassay_007086_13	euxassay_007086_14

euxassay_007086_15	euxassay_007086_16	euxassay_007086_17	euxassay_007086_18	euxassay_007086_19

euxassay_007086_20	euxassay_007086_21	euxassay_007086_22	euxassay_007086_23	euxassay_007086_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11829_wholemount_strong.wlz

11829_wholemount_moderate.wlz

11829_wholemount_weak.wlz

11829_wholemount_possible.wlz

11829_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11829_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4597
Entity Detected:	Plk3, polo-like kinase 3 (Drosophila) ( MGI:109604)
Sequence:	sense strand is shown >T4597 GAAACCAGGCAGCGCCACGCGCGGCCGGGGCCGGGCGGAACCGAGAACCAGCTCGGCAGGCGCTGCGCCG CGCCGTCCGCATGGAGCCCGCCGCCGGCTTCTTGTCTCCGCGCCCCTTCCCGCGCGCGGCTGCCCCGTCC GCGCCCCCCGCCGGCCCGGGGCCGCCTGCCAATGCCTCCCCGCGATCGGAACCTGAGGTGCTGGCCGGGC CACGGGCGCCGGATCCTCCTGGTCGCCTCATCACCGACCCTCTCAGCGGCCGTACCTACACCAAGGGTCG CTTGTTGGGCAAGGGGGGCTTCGCACGCTGCTATGAAGCCACTGACACCGAGTCTGGTATAGCCTACGCG GTCAAAGTCATCCCGCAGAGTCGCGTCGCCAAGCCGCATCAGCGCGAGAAGATCCTAAATGAGATAGAGT TGCACCGAGACCTACAGCACCGCCATATCGTTCGCTTTTCACATCATTTCGAGGATGCTGACAACATATA CATTTTCCTGGAGCTCTGTAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGACACACCCTGTTGGAA CCAGAGGTTCGCTACTACCTGCGCCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGAGGGGTATCTTAC ACAGAGATCTCAAGCTGGGAAATTTTTTCATCACGGATAACATGGAACTGAAGGTGGGGGATTTTGGGCT GGCAGCTCGATTAGAGCCCCCAGAGCAGAGGAAAAAGACCATCTGTGGCACTCCCAACTACGTGGCTCCA GAAGTGCTGCTGAGACAGGGCCATGGCCCCGAGGCAGATGTGTGGTCGCTTGGTTGTGTCATGTACACGC TCCTGTGTGGGAGCCCCCCATTTGAGACTGCTGACCTGAAGGAGACATACCGCTGCATCAAGCAGGTTCA TTACACGCTACCTGCCAGCCTCTCCCTGCCTGCCCGGCAGCTCCTGGCTGCCATCCTCCGAGCTTCACCC AGAGACCGCCCTTCCATTGAACAGATTTTACGCCATGACTTCTTTACGAAGGGCTACACCCCTGATCGGC TCCCAGTCAGCAGCTGTGTGACAGTCCCAGATCTGACACCCCCCAACCCTGCGAGGAGTCTGTTTGCCAA AGTTACCAAGAGCCTGTTTGGCAGGAAAAAGAACAAGAATAAGAACCATTCTGAGGACCAGGATAATGTC TCCTGCTTGGTGAGTGGCCTCATGCGCACATCCATCGGTCATCCAGATGTCAGGCCGGAGGCTCCTGTAG TTTCAGGCCAGGCCCCTGCCAGCTTGATAGAGACAGCTGCCGAGGACAGCTCGCCCCGTGGGACACTGGC CAGCAGTGGAGATGGGTTTGAAGAAGGTTTGACTGTGGCCACAGTGGTAGAGTCTGCCCTCTGTGCACTG AGAAACTGTGTGGCCTTCATGCCCCCAGCCGAACAGAACCCAGCCCCACTGGCACAGCCAGAGCCTCTGG TGTGGGTCAGCAAGTGGGTTGACTACTCCAACAAGTTTGGCTTTGGCTATCAGCTGTCCAGCCGCCGTGT AGCCGTGCTCTTCAATGATGGCACACACATGGCCCTGTCAGCCAATAGGAAGTAAGTGCTGTTTTGGCGT GCTTTGTAATTAGGCTTGCTGGGTACCACAATACCAGCAGGGTTGCACCGTGGTGTGTGTTTCTGTGCTT AGGGGTCTGGGGAAGAATGGTGGTGCCGGGTTCCTCCTCCTCCTCTCTACGTTCCCACTTTCAGGACGGT GCACTACAACCCCACCAGCACGAAGCACTTCTCCTTCTCCATGGGTTCTGTGCCCCGAGCTCTGCAGCCT CAGCTGGGCATCCTGCGGTATTTTGCGTCCTACATGGAACAGCACCTCATGAAGGGTGGAGATCTGCCCA GTGTGGAAGAGGCGGAGGTGCCTGCCCCACCCTTGCTGCTGCAGTGGGTCAAAACTGACCAGGCCCTCCT CATGCTGTTCAGTGATGGCACTGTCCAGGTGAACTTCTACGGGGACCACACGAAGCTGATCCTCAGTGGC TGGGAGCCTCTCCTTGTGACCTTCGTGGCCCGCAATCGCAGTGCTTGTACTTACCTCGCTTCCCACCTCC GGCAGCTGGGCTGCTCCCCAGACTTGCGGCAGCGACTGCGCTACGCTCTGCGCCTGCTCAGGGACCAAAG CCCTGCTTAGGTTCCAACTCGGAGAGCGGGCTGTGATCCAGATCCTGGGCCTGAGCCTGAAAGGCTTCAG GGCCTTGCCTTTGTGGCCCTTCCTGTCCCTTTGGTGCCTCACTGGGGGCTCCTGGCTGAATCCCCCCAGG GAATCAGGGACCAGCTTTACTACAGTCGGGGATGGCCTGCCCTCCCTGAATCTTACCCTTCTCTCTGAGA TAAGCCTGAGCCTTAGCTCCCAGCTAGGGGGCATTATTTATGGACCACTTTTATTTATTAACAGACATTT ATTTATTGGGATGTGAGCCCCAGGGGGCCTCCTCCTGGGGTAATAAACCGTTTTTTCAGAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4988360 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4988360 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11826 same embryo
	EMAGE:11825 same embryo
	EMAGE:11827 same embryo
	EMAGE:11824 same embryo
	EMAGE:11828 same embryo
	EurExpress:euxassay_007086 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source