EMAGE mouse gene expression spatial database

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EMAGE:11831

Drd5 dopamine receptor D5 ( MGI:94927)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007160. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007160_01	euxassay_007160_02	euxassay_007160_03	euxassay_007160_05

euxassay_007160_06	euxassay_007160_07	euxassay_007160_08	euxassay_007160_09	euxassay_007160_10

euxassay_007160_11	euxassay_007160_12	euxassay_007160_13	euxassay_007160_14	euxassay_007160_15

euxassay_007160_16	euxassay_007160_17	euxassay_007160_18	euxassay_007160_19	euxassay_007160_20

euxassay_007160_21	euxassay_007160_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11831_wholemount_strong.wlz

11831_wholemount_moderate.wlz

11831_wholemount_weak.wlz

11831_wholemount_possible.wlz

11831_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11831_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 08 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 15 16

neural retina

weak

regional

weak expression: see section 01 02 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35154
Entity Detected:	Drd5, dopamine receptor D5 ( MGI:94927)
Sequence:	sense strand is shown >T35154 GAACTGTATGGTTCCCTTCTGCAGCAGTGGAGATGCCCAGGGCCCAAGGACTGGCTTCCCTTGTGTCAGC GAGACCACCTTCGACATATTCGTCTGGTTTGGCTGGGCCAACTCCTCTCTCAACCCCATCATCTATGCCT TTAATGCAGACTTCCGGAAGGTGTTCGCCCAGCTGCTGGGGTGCAGCCACCTCTGCTTCCGGACCCCCGT GCAGACGGTAAACATCAGTAATGAACTTATTTCCTACAACCAAGACACGGTCTTCCACAGGGAGATCGCT GCTGCCTATGTCCACATGATCCCAAATGCGGTGTCCTCCGGAGACAGGGAGGTGGGAGAGGAGGAGGAGG CGGAGGAGGAGGGGCCTTTCGATCACATGTCTCAAATCTCTCCAACGACCCCAGATGGTGACCTGGCTGC TGAGTCTGTCTGGGAGCTTGACTGTGAGGAGGAGGTTTCCTTAGGCAAAATCTCACCACTCACCCCCAAT TGTTTCCATAAAACTGCTTAGAAACACCCTCATGGGCATGTACAGTTGTGGCCATATTTACAAGCATGCA CAAATGCGCGCGCACACGCACACAAATATACACACTCCTGGTGTGCTTATGCTTTCTGTAGTCCTGCTGC GTAGAAACCAAACGATTCTTAGCTGAGAAAATGAATGAGCAGAGGCTGTTGGAATAAACTCAGATATTCT GGGCACAGAAGAGAGTTCCCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90586. Forward Primer - name:090586_F_cDNA_Drd5, sequence:GAACTGTATGGTTCCCTTCTGC; Reverse Primer - name:090586_N_SP6_cDNA_Drd5, sequence:AAGGGAACTCTCTTCTGTGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11830 same embryo
	EurExpress:euxassay_007160 same experiment
	MGI:4824394 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source