EMAGE mouse gene expression spatial database

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EMAGE:11837

Pbld2 phenazine biosynthesis-like protein domain containing 2 ( MGI:1914557)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006909. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006909_01	euxassay_006909_02	euxassay_006909_03	euxassay_006909_04

euxassay_006909_05	euxassay_006909_06	euxassay_006909_07	euxassay_006909_08	euxassay_006909_09

euxassay_006909_10	euxassay_006909_11	euxassay_006909_12	euxassay_006909_13	euxassay_006909_14

euxassay_006909_15	euxassay_006909_16	euxassay_006909_17	euxassay_006909_18	euxassay_006909_19

euxassay_006909_20	euxassay_006909_21	euxassay_006909_22	euxassay_006909_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11837_wholemount_strong.wlz

11837_wholemount_moderate.wlz

11837_wholemount_weak.wlz

11837_wholemount_possible.wlz

11837_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11837_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

kidney calyx

moderate

regional

moderate expression: see section 10 21 22 weak expression: see section 11 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9926
Entity Detected:	Pbld2, phenazine biosynthesis-like protein domain containing 2 ( MGI:1914557)
Sequence:	sense strand is shown >T9926 GAGCAGGGCAGGGAGCCCTCAGACAGAGCCAGCGAGGAAAATGAAGCTCCCAATCTTCATAGCAGATGCG TTCACTGCGACAGCTTTCCGGGGCAATCCTGCCGCAGTCTGCCTCCTGGAGCGCACATTGGATGAAGATG CTCATCAGGACATTGCAAGGGAAATGAACCTCTCTGAAACCGCTTTTGTCCGCAAACTGCAACCAACTGA TGACTTCACACAAAGTTCCCGTTTTGGGCTGAGGTGGTTTACCCCAGAGGCCGAGTTCCCCTTGTGTGGC CACGCCACGCTGGCTTCTGCAGCTGTGCTGTTTCAAAAAAGAAAGAACACGAACAGCACCCTGACCTTTG TCACAATGAGCGGGGAGCTAAAGGCCAGAAGAGAAGAAGATGGGATCGTCCTGGACTTTCCTGTCTACCC AACCTTCCCCCAGGACTTCCATGAAGTTGAAGACTTGATAAAGGCAGCCATAGGTGACACCCTGGTCCAG GACATCCGGTACTCTCCAGATACCAAAAACCTCCTGGTCCGGCTCAGCGATTCTTATGACAGGTCCTTTC TAGAGAGCCTGAAGGTGAACACAGAGCCTCTGCCTGCAATTGAGAAGACAGGGAAGGTGAGAGGCCTCAT CCTCACTGTCAAAGGAGAGCCTGGGGGGCAGACAGCCCTGTATGACTTCTACTCCAGATGCTTCGCGCCG TGGGTTGGTGTGGCTGAAGACCCGGTGACAGGGTCCACCCACACTCTTCTTGGCCCCTACTGGTCTGAGG AGCTGGGGAAGAAGGAAATGCGAGCCTTTCAGTGTTCCCGCCGAGGAGGGGAGCTGGACATTAACCTGCG ACCTGATGGGCGCGTTGACATAAAGGGCGGAGCTGTTATTGTCTTGGAGGGCACGCTGACCGCCTAGAGA TGTTCCTGCTGGGCTTGGGGCCACTGTTCTACATGGTGTCTTCTCTAGAGCGCAGAACCCAACCCAACTC AACAGCAAATGCACAGAGCTTGGTTCAGTCGTCGTGTGAACCCAAAAGGGAAGACACCTGATGGAGGTGC GGTAGTGTCTCCAGAATCCTTTTCCTGATGGTGGGCTCTGAGCTCTGCCTGTGTGTGCATCTTCAGTCCC CACAGCAGATAGGGATGCTGCCCCTTCTTTTGACCATGACTGCCAATCAAAGTGAGAGGTGCATTGATAA GGAACGAGAAGAAATTTAGAATGATAAAGGCATTGACACGAAATGAGAAGAAATTTAGACATATGTGTGG GGGCCTTATAGATGATTTATTTTCTGAGACAGGATTTCTCTGTGTAGCTCTGGCTGTCCTCTAACTCACA GACATCTGCCTGCTTCTGTCTCTAGTGTGTTGGATTAAAGGCACGTGCCACCATCAAATGGTTTCTGATA GATTTTACAAATTGTGTGTGTGTGTGTGTGTGTGTGTAATTTTAATTTAAAGACAGGGTCTTACTGTATA GCCGTGGCTGGGAAGAAACTCTCTATGTAGACCAAGCTAGCCTCCAACTCGACCCCTGCCTACCTCTGCG TCTCCAGTGCTGGGTTAATGCTATGCACCATCATGCCCAGAGTCACAATCATTGTAATCGACCCAATAAC TCTGTGGAAATGCAGGCGCAGTACTAAAGACAGTAGACTGTAAACTGTCCCAGGGGCTTCACTTGGGCTC TGTGGAATATTGATTCACGCGACTGTGGAGACAGCCAGGGGAGACAGTTCATGTGGAAAGACTCAGAGGA GGCCTAGTGTTTAGGAAACAAATCCTTTGTATTTTGAACTCAGCTAGGAGTAACAATCAGGACAAACCCA GGAGATTACAACAGTAGGTATCTGATTTGGTGATGTGATGATCAAAATACACGCTAAGGGCGTGTGAGAC GAAATCTTTCCCTCCTGTGTGGCTTAGAATGGCCATGTCATTGTCTTCTCTTGTTTAATAAAGCGTGATT TAATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30381600 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30381600 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11838 same embryo
	EMAGE:11839 same embryo
	EurExpress:euxassay_006909 same experiment
	MGI:4822701 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source