EMAGE mouse gene expression spatial database

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EMAGE:11848

Gpatch2 G patch domain containing 2 ( MGI:1915019)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008536. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008536_01	euxassay_008536_02	euxassay_008536_03	euxassay_008536_04

euxassay_008536_05	euxassay_008536_06	euxassay_008536_07	euxassay_008536_08	euxassay_008536_09

euxassay_008536_10	euxassay_008536_11	euxassay_008536_12	euxassay_008536_13	euxassay_008536_14

euxassay_008536_15	euxassay_008536_16	euxassay_008536_17	euxassay_008536_18	euxassay_008536_19

euxassay_008536_20	euxassay_008536_21	euxassay_008536_22	euxassay_008536_23	euxassay_008536_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11848_wholemount_strong.wlz

11848_wholemount_moderate.wlz

11848_wholemount_weak.wlz

11848_wholemount_possible.wlz

11848_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11848_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9286
Entity Detected:	Gpatch2, G patch domain containing 2 ( MGI:1915019)
Sequence:	sense strand is shown >T9286 GTCCTGTGTGGAATTGGCCGGGGCTGCGGGAGTTGGAGTCGGTCCTCGGGCCTGAGCCTCGAGGCCGCGC TCCCGGGTGTCGTTGATGTTTGGGGCCGACGGGCGCCCAGCGATCGGAACCGCCGCCGGGAAGAGCTGGC ATTTCAGTCGAACAATGGAAGAGCTTGTTCACGATCTTGTCTCTGCACTGGAAGAGAGCTCTGAGCAAGC CCGAGGTGGATTTGCTGAAACTGGAGAACATTCTCGAAATCTGTCTTGCCCTCTGAAACGCCAGGCCCGG AAAAGGAGAGGGAGGAAGCGGAGATCCTACAATGTTCACCACCCGTGGGAGACAGGCCACTGCTTAAGTG AAGGCTCTGATTCTAGTTTAGAAGAACCAAGTAAGGACTATAGAGAGAAGCACAGCAATAATAAAAAGGA CCGCAGTGACTCTGATGACCAGATGTTAGTGGCGAAGCGGAGGCCATCTTCAAACCTAAGCAGCAGCGTT CGAGGTAAGCGGCTTCTGTGGCACGAGTCTGACTTTGCCGTGGACAGCCTTGGGAACAGAACGCTGCGCC GGAGGAGGAAGGTGAAGCGCATGGCCGTGGATCTCCCGCAGGACGTCTCCAGCAAAAGGACAATGACCCA GCTGCCAGAAGGCTGCAGAGATCAGGACATGGACAATGATAGAGCCAGCCAGTATCCAGAGTTTACCCGG AAGAAAGTTAAGAAAAGGAAGTTGAAAGGGATTAGGCCAGGACCGAAAACCCAGGAGGAAGGAGGAGTTT TGGAGAGTGAAGAAAGAAGCCAGCCCAACAAGGACAGGATGGAGTACGAGGAACAGAAAGCCTCGGATGA GCTCAGGAGCGAAAGTGACACCAGCAGTCTCAGCAGCACTGACGCGGGCTTGTTCACCAACGATGAGGGA AGACAAGGTGATGATGAGCAGAGTGACTGGTTCTATGAGAAGGAGTCAGGCGGAGCGTGCGGGATTGCTG GAGTCGTGCCCTGGTGGGAAAAGGATGAGCCAGCAGAGCTGGACACCAACCTGCCCGACCCTGTGTTTGA GAGCATCTTAAGTGGCTCCTTCCCTCTCATGTCCCATCCTGGCAGAGGAGGTTTCCAAGCTAGACTCAGT CGCCTTCATGGAACGCCTTCAAAGAATATTAAAAAGTCTTCAGGGGCTCCACCTTCAATGCTGCCTGCTC CTGGCCCTGGCAGTAACAAGAGGATGGTTCACTTCTCCCCAGACGCCCATCGCCATGACCACTGGTTTAG CCCTGGGGCTAGGACAGAGCACGGCCAGCATCAGCTTCTGAGAGATAACCGAGCGGAAAGAGGACACAAG AAAAGCTGCTCCCTGAAAACAGCCAGCAGGCAGACAAGCATGCATTTAGGATCCTTGTGCACAGGAGACA TCAAAAGGAGAAGGAAAGCTGCCCCGTTGCCTGGACCCACTGCAGCAGGAATTGTGGGTGAGAACGCCCA GCCGATCCTAGAGAGCAACATCGGGAACCGCATGCTTCAGAGTATGGGATGGACACCCGGGTCAGGCCTC GGGCGAGATGGCAGAGGGATCGCGGAGCCAGTTCAAGCCGTTCAGAGGCCGAAAGGGTTAGGACTTGGAT TTCCTCTACCAAAAAGCTCCCCCACCAGCCCTGCCCCCACATCAGGAAACCCTGCCTGAGCAGAGAGAGA ACCTGCGGTTTCTGTTGCTGTGCCCCATTGTTATAACATGATCGTAGTGTGTACTGGTTTGAAGCCGACC TCTGACGTTGCCTCCAGCTAGCTTCCAGGCTGCATAGCGTCCCGCCAGTGATAGAAATGGGATTCCATCA CAACTCACGGTGCTCAAGTACGAACCTCAACCCTTCACGTGTTTCCTCGGTTTCCAGCAGTTTCTAGACA TGAAGAATAGTTGGTCTCTTATTTATTGTGATCCTAGACGTCAGTTCTTTCTGCTGCCTTGCTCCATCCG TGCGTGTACGTGATGCTCAGACACAGTGGAGCACACCCAGTGGGTCTGAGGATCACATTCTTATGATGCC GACACTAAGTAGTGTGGTCCTCCTCTCTCCCACGTGCTCCCTCTCCCACATGCAGGGAGTCCTAAGCATG TCCGGGAAGGCTTGCTTAGTGAGCTCACTTCCCTTTGCTTTGGCTCTGATGAGGGTTTGTAACTGGGATG CCGTTAACTGTTTTCAGCAGTGTAGCATAAATTCACAGTGGGATGTTTGGCAAGAGCTAACGCCTTTGTC GTCATCGTCGTCATGAGAGCACTGTCAGCATCACTCCAGTGCAGAGCTTACACACCTTACCTCATTTAGG ACGTAAAGTCATCGCTAAAAGAAAATGTATTTTTAAAGTCATCCTGACTGACCTGCAAACCCTGCCCTCC ATATGTCTACCTCTCCCCAAAGGCTGGGGTTAAGGATCAGCTCCAAGAGGAAGAAGAGTGGCCTGCTCAG GGTTTAGTCACTGAAGTAAATTAGTAAACTCCTGAGTCAGTTTGTTTGAAGATATAAATAGCTTTTCCCA AAACTGAGCTAACTGGCCTAACCCACTTACCAAGTACAGAAGGAACAGAGGGACCAGGCATTCTGATACT AAACACTTAGTTTAAAATTAAGCGAAACACTATGTTCCTTCCATTCCATTCTGTCGTTCTCTCCATAAGG TCAGAAATTCTAAGTTGTTCTTTGTTCAGAAAATACTGATTTATTCTAATGTTCATTAAAAAACCCTAAT TATTATTACTTAGAATGGCTCCTTTGCTTCAGAAAAGGTTATGAGAACCCATACTAGTGTATGCTTGAAC AAAGCAGTATGAACTTCAATTTTGTAAATTGAAAAAGAAATTAAAATATTTAAGGCAATAGAAAAGGTTT CAGCAGGTTAACAGACCTTTCCACGTATGGTCCTCTATAAAAAATGCAGACTCTGTAGTCTGGCAAACAT CTGCATTTCTTCTAGGAACTGTCTTTCCTGAGAAGTGGCCGTGGTAAGAAGTGGGCTGGCCAGGACACAA GTGGACCCCGTTTGTGCCTCACGGAACACTTGTGTTGGGGAAAGCATTGCTGCTGGAACAAGCTCGTGAC TAGTAAACTGCCATTTTTAATAGCTGTCTTTCTAAACGCTTACCATATTCACGTAGGAGGGAAGATCGAA GAGACTAACCGTGTACTGGACTCAAGGGTCTTTTCCAAACTTGTTTGCTCTTCTTTGAGTTTCATTTCTT GATGGTCTTGTAAAGCCCTAGGAGGGGACTTTTAAATATAAGCTCAATTTCAAGCCAGCGAGAAACAGTG AGGCCACTGAACGCTTGGCTCCAGTCTCCAGAGAAATCAGCTGCTGTGTCCTTACCCTGCCCTAGAGGAT GCACAGGAACGGCCATGTGCTGAGCACTGGGCCTCCAAAACTGCTCTCTCTGGGCACTTCCCATCACTGC CCTCCAGACAATGTGAACCCTCTGCTGCAGCTTCTGCAGCAAGGTCCTCACGAGCTGCTGGGACACCACA GTTCTACTTTCACACAAGAAAGCTCCTTACTCACAGGGTTCATCTTCTACTAGTGTTTATCTGTTCAGAG TGGATGACCAGCTCTTACAGAAGAACCGACAGGCCCCAGCCCACCGCCAGCTGTCCACAGGTACCCTTCA GCATTCAAGTCCTTATAAAGAGCAGCAAGATGTGACGTCCCTGGCATACCTGACCATAGCTGAGAAGTAA ACACAATTAAAATAGGAAATGTTCATGTAAATCAGATGTCTGCTCGGGACATGAATCCCACTGGTTTACA TTTAATAGAGATTAATAAATCATTATCAGTTCAAATCAGTTCATGGTGCCCACATCATTGGAGTTCATGA TTTTAACTCTATCATTCCTATCACCTTTAAATAGTCTTAAGAGATTTCTTCTACTTCCTCATGAGTCCAC CCTGTTGTTCTGCAAGCCAGTCAGTTAGGGTCTCTTCCCTTCCCATCCACTCCCTACGGTAGCCAAAGAA TCGGAGCATAGCTGTGTCAGCAGTGTGACTCCGCATTGTGTGTCTTTAATGTCAACCAGGTGTGGGCTGT AGTCCTTTCCTCCCTTCTTCCCTCCCCTAAATAGAATGTATAAGGCTGAATTCCCTTGAATTCATTTCTT TTTGTTTTTAAATGATCTAGTGACTTTTAAAGTGGGCAGCTATGCTAACACCCACCCAGAAGTTGGAAGA TTGAGGCAGGACTGCCGTAAGTCCAAGACCAACCTAGACTGTGTAGTGAGTTTCAGGACAGATGGTGGCA TATGGTGATACCCGTGTCAAAAGTAAATATCAATGGAAACCACGTTAGTAAGTGCCCCTTACCTGGAAAT TGGGCACCGTGTTTGAGCACAGCAGTTTGGGACTGGCTGCAATGATTATGCAGACAGAGTTGAGACAGCA GGTTAAACATGCGTGCTTGAGTATGTGTGCACGCGGGTGCACTGCAGCCGTGTTAGTTTGCGGTGGACAG ACAGGATTGAGAGGTTCTGCCCTTCATTCATGTTTAACCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6839419 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6839419 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11850 same embryo
	EMAGE:11851 same embryo
	EMAGE:11849 same embryo
	EurExpress:euxassay_008536 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source