EMAGE mouse gene expression spatial database

EMAGE:11853

Dchs2 dachsous 2 (Drosophila) ( MGI:2685263)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009580. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009580_01	euxassay_009580_02	euxassay_009580_03	euxassay_009580_04

euxassay_009580_05	euxassay_009580_06	euxassay_009580_07	euxassay_009580_08	euxassay_009580_09

euxassay_009580_10	euxassay_009580_11	euxassay_009580_12	euxassay_009580_13	euxassay_009580_14

euxassay_009580_15	euxassay_009580_16	euxassay_009580_17	euxassay_009580_18	euxassay_009580_19

euxassay_009580_20	euxassay_009580_21	euxassay_009580_22	euxassay_009580_23	euxassay_009580_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rest of cerebellum mantle layer

weak

regional

weak expression: see section 04 05 06 17 18 19

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 21 22 23 weak expression: see section 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36266
Entity Detected:	Dchs2, dachsous 2 (Drosophila) ( MGI:2685263)
Sequence:	sense strand is shown >T36266 GGAGAAACACGTCTCTTTTGCTGCTGTGCATATCAGTGTGTTAGATGACAATGACCACTCACCTCAGTTT CTGTCCTCTACCTTGGCCTGCATCACTCCTGAAAACCTGCCTCCTCTCTCCATCATATGTTCTGTGCACG CTCTGGATTTTGACACCGGGCCTTACGGAGAGGTGACTTACTCTATTGTGTCACCCTGCCTTGTCACACA CGGGATGCATCCTTATCAGGATCTCTTTGCCATTGATCCTTTGACAGGGGACATTCACACTGAGCAAATG CTTGACTATGAGAGTGTCAGGGAGTACTGCCTCCTGGTTCAGGCCAAAGACAGAGGTGATGCCTCAGCTT CTCTGGAGGTTTGGGTGGAAGTTGAAGGGATAGACGAGTTTGAACCCATTTTTACTCAAGACCAATATTT TTTCAGTCTCCGTGAGAAGGGTCAAGGACAGCAGCTGATTGGCAGAGTAGAGGCCTCGGACGCGGATGCT GGGGTGGATGGGGAAGTGCTCTACTCTCTTAGGACTCCATCCACTGTCTTCTCGGTCAACAAAACGAATG GCAACATTTACTGGGTTAGAGCTCCTCTCCTAGGAAGCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77282. Forward Primer - name:077282_F_cDNA_Dchs2, sequence:GGAGAAACACGTCTCTTTTGCT; Reverse Primer - name:077282_N_SP6_cDNA_Dchs2, sequence:TGCTTCCTAGGAGAGGAGCTCTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11857 same embryo
	EMAGE:11855 same embryo
	EMAGE:11854 same embryo
	EMAGE:11856 same embryo
	EMAGE:11852 same embryo
	EurExpress:euxassay_009580 same experiment
	MGI:4824248 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source