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EMAGE:11856

Dact1 dapper homolog 1, antagonist of beta-catenin (xenopus) ( MGI:1891740)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856
"Pseudo-wholemount" of euxassay_009577. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009577_01 euxassay_009577_02 euxassay_009577_03 euxassay_009577_04
EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856
euxassay_009577_05 euxassay_009577_06 euxassay_009577_07 euxassay_009577_08 euxassay_009577_09
EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856
euxassay_009577_10 euxassay_009577_11 euxassay_009577_12 euxassay_009577_13 euxassay_009577_14
EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856
euxassay_009577_15 euxassay_009577_16 euxassay_009577_17 euxassay_009577_18 euxassay_009577_19
EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856 EMAGE:11856
euxassay_009577_20 euxassay_009577_21 euxassay_009577_22 euxassay_009577_23 euxassay_009577_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11856Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11856_wholemount_strong.wlz
11856_wholemount_moderate.wlz
11856_wholemount_weak.wlz
11856_wholemount_possible.wlz
11856_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11856_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
adrenal cortex
moderate moderate
regionalmoderate expression: see section 06 07 08 09 17 18
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 19
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 17 18 weak expression: see section 15 16
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 17 18 weak expression: see section 15 16
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 17 18 19 weak expression: see section 15 16
pons mantle layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 17 18 19 weak expression: see section 15 16
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15
aorta
moderate moderate
regionalmoderate expression: see section 11
esophagus
moderate moderate
regionalmoderate expression: see section 12 13
stomach mesentery
moderate moderate
regionalmoderate expression: see section 09 10 11
midgut
moderate moderate
regionalmoderate expression: see section 13 14 15
duodenum caudal part mesentery
moderate moderate
regionalmoderate expression: see section 13 17
jejunum mesentery
moderate moderate
regionalmoderate expression: see section 13 17
midgut loop mesentery
moderate moderate
regionalmoderate expression: see section 13 14 15 17
rest of midgut mesentery
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17
urinary system
moderate moderate
regionalmoderate expression: see section 13 14 15
renal capsule
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 17 18 19
renal medullary interstitium
moderate moderate
regionalmoderate expression: see section 08 09 10 17 18
trachea cartilaginous ring
moderate moderate
regionalmoderate expression: see section 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36261
Entity Detected:Dact1, dapper homolog 1, antagonist of beta-catenin (xenopus) ( MGI:1891740)
Sequence:sense strand is shown

>T36261
GACGTATATCGCTACCCCAGTCCACTTCATGCTGTGGCTGTGCAGAGCCCAATGTTTCTCCTTTGTCTGA
CGGGCAACACTCTGAGGGAAGAGGAGGGGCTTGGGAGCCATGCCAGCGACATCTGCATTGGATCTGAACT
GAACGCCACCAAAACAGACAATTCCCTGCCATCTCCAAGCAGTTTGTGGTCCGCTTCCCATCCTGCATCC
AGTAAGAAAATGGATGGGTATATTTTGAGCCTCGTGCAGAAGAAAACACACCCTGTAAGGACCAATAAAC
CTAGAACCAGTGTGAACGCTGACCCTACCAAGGGCCTTCTGAGGAATGGAAGTGTGTGTGTCAGGGCCCC
TAGTGGCGTCCCACCGGGCAGTAGTGTGAACTTTAAGAATACAAAACAGATGTGTTTGCCCGCTGGGGGA
ATAACCTCTTTGGAAAACGGGCCATTCTCCCCTCCTAAGCAGAGGTCCAAAGACTCAAAGACAGACCAGT
TAGAAAGCAAGAGGTTGGCTCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93069. Forward Primer - name:093069_F_cDNA_Dact1, sequence:GACGTATATCGCTACCCCAGTC; Reverse Primer - name:093069_N_SP6_cDNA_Dact1, sequence:CAGAGCCAACCTCTTGCTTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11857 same embryo
 EMAGE:11855 same embryo
 EMAGE:11854 same embryo
 EMAGE:11852 same embryo
 EMAGE:11853 same embryo
 EurExpress:euxassay_009577 same experiment
 MGI:4824221 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS