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EMAGE:11858

Fam71e2 family with sequence similarity 71, member E2 ( MGI:3045311)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858
"Pseudo-wholemount" of euxassay_011068. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011068_01 euxassay_011068_02 euxassay_011068_03 euxassay_011068_04
EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858
euxassay_011068_05 euxassay_011068_06 euxassay_011068_07 euxassay_011068_08 euxassay_011068_09
EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858
euxassay_011068_10 euxassay_011068_11 euxassay_011068_12 euxassay_011068_13 euxassay_011068_14
EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858
euxassay_011068_15 euxassay_011068_16 euxassay_011068_17 euxassay_011068_18 euxassay_011068_19
EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858 EMAGE:11858
euxassay_011068_20 euxassay_011068_21 euxassay_011068_22 euxassay_011068_23 euxassay_011068_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11858Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11858_wholemount_strong.wlz
11858_wholemount_moderate.wlz
11858_wholemount_weak.wlz
11858_wholemount_possible.wlz
11858_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11858_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37354
Entity Detected:Fam71e2, family with sequence similarity 71, member E2 ( MGI:3045311)
Sequence:sense strand is shown

>T37354
CCTGTCACGGTTTCCTCTAATCAACTGGACACCCTCCTGTGGGCACAAAACCTTGAAGATCTCATGAATC
CTGAATATGCCACACTGTCTTCCTTGAACACATCTCACCCTCCTGCCTTCTACATCTTTCCTCCCAATCC
CTCCTTTCTGAAGTCCAAAGACAAGGCCAGACCCACAGGCTCAAAGAAACACGTGAGCCCACTGTCTTCC
CACAAAGCTGCATGTGTCCCCATCGAATCGAGGAAAATGCCATTCATTTTGGACCAGTCTAACAAGGTAC
CGGCTAAGCCTGCTCCAGCTCAGATGGCCTCAGCCCTTCCCACTCCGTCTCGGAAGACCCCGACTGCACT
GTGGTCAAGAGGGAAGGCTTCAGGTGCACTTGGCCATTTCTTTAAGTCTCAGCCTTCTGTTTCCCAGAAG
ACCCCAACCACCCCTGGGCAGCCCTTGAAGCCCCAAGTTATAACTACCCCAACCCAGAAAATTTTAACAC
CGAATCAGAAGCTTCTACAAGCACCTGCACATTCCCAGAAAGCCATGCACACCAAAAAGGACCATCTAAT
TGTAAATATCCGGTCTCAGAAGGATCCAACTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67722. Forward Primer - name:067722_F_cDNA_4930401F20Rik, sequence:CCTGTCACGGTTTCCTCTAATC; Reverse Primer - name:067722_N_SP6_cDNA_4930401F20Rik, sequence:GCAGTTGGATCCTTCTGAGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11861 same embryo
 EMAGE:11859 same embryo
 EMAGE:11860 same embryo
 EMAGE:11863 same embryo
 EMAGE:11862 same embryo
 EurExpress:euxassay_011068 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS