EMAGE mouse gene expression spatial database

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EMAGE:11869

Csn1s2a casein alpha s2-like A ( MGI:88542)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011051. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011051_01	euxassay_011051_02	euxassay_011051_03	euxassay_011051_04

euxassay_011051_05	euxassay_011051_06	euxassay_011051_07	euxassay_011051_08	euxassay_011051_09

euxassay_011051_10	euxassay_011051_11	euxassay_011051_12	euxassay_011051_13	euxassay_011051_14

euxassay_011051_15	euxassay_011051_16	euxassay_011051_17	euxassay_011051_18	euxassay_011051_19

euxassay_011051_20	euxassay_011051_21	euxassay_011051_22	euxassay_011051_23	euxassay_011051_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11869_wholemount_strong.wlz

11869_wholemount_moderate.wlz

11869_wholemount_weak.wlz

11869_wholemount_possible.wlz

11869_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11869_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 18 19 20 21 22 23 24

forebrain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 08

hindbrain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 08

midbrain

moderate

homogeneous

moderate expression: see section 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 08

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 17 moderate expression: see section 08 09

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 17 18

trigeminal v nerve

strong

regional

strong expression: see section 09 10 17 18

spinal cord

moderate

homogeneous

moderate expression: see section 07 09 10 11 12 13 14 15 16 17 18 weak expression: see section 08

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 10 15 16

cervical ganglion

weak

regional

weak expression: see section 09 16

thoracic ganglion

weak

regional

weak expression: see section 14 15

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 15 16 17 18 19

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 23 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 15 16 17 18 19 20

vomeronasal organ

weak

regional

weak expression: see section 13 16

mandible

weak

regional

weak expression: see section 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23

maxilla

weak

regional

weak expression: see section 06 07 08 09 10 11 16 19 20 21 22 23

clavicle

moderate

regional

moderate expression: see section 09 17 weak expression: see section 08 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36207
Entity Detected:	Csn1s2a, casein alpha s2-like A ( MGI:88542)
Sequence:	sense strand is shown >T36207 GAAGTTCTTCATCTTTGCCTGCCTGGTGGTTGTTGCTCTGGCAAAGCACGAAATAAAGGATAAGTCCTCC AGTGAGGAATCATCTGCCAGCATCTACCCAGGAAAGTCTAAGCTGGACAATAGCGTGTTCTTCCAAACAA CTAAGGATTCTGCTAGCAGTTCATCCAGTGAGGAATCTTCCGAGGAAGTCAGTGAAAAAATTGTACAATC TGAAGAACAAAAGGTCAACCTAAACCAGCAGAAAAAATTCAAGCAATTTTCCCAGGAGTCTTCCTTTTCC CAATGTTGCACACCTCTTCACCAGCAGCAGCAGAGCAGTGTGAACCAGTGGCCCCAGCCTAATGCCATCC ATAACACGCCCACCCAGGAATCCATCAGCACCTCTGTGGAGGAAATCCTGAAGAAGATTATTGATATGAT CAAGTACATCCAATATCAACAGGTCACCATCCCCCAACTTCCCCAAGCTCTTCATCCCCAGATACCTGTG AGCTACTGGTACCCCAGTAAGGATTACACCTTCCCAAATGCTCACTATACGAGATTCTACTAAGATTCCT AAAATAACTGCTGCTACCTAGCTATAGGCTTGAGTAGAAAATTGGTTTCTTCGTGGTTTCCCCATCACCT CCTACTATTAACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97599. Forward Primer - name:097599_F_cDNA_Csng, sequence:GAAGTTCTTCATCTTTGCCTGC; Reverse Primer - name:097599_N_SP6_cDNA_Csng, sequence:TGTTAATAGTAGGAGGTGATGGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11870 same embryo
	EMAGE:11872 same embryo
	EMAGE:11868 same embryo
	EMAGE:11871 same embryo
	EMAGE:11873 same embryo
	EurExpress:euxassay_011051 same experiment
	MGI:4824082 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source