EMAGE mouse gene expression spatial database

EMAGE:11871

Krt71 keratin 71 ( MGI:1861586)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011058. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011058_01	euxassay_011058_02	euxassay_011058_03	euxassay_011058_04

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euxassay_011058_10	euxassay_011058_11	euxassay_011058_12	euxassay_011058_13	euxassay_011058_14

euxassay_011058_15	euxassay_011058_16	euxassay_011058_17	euxassay_011058_18	euxassay_011058_19

euxassay_011058_20	euxassay_011058_21	euxassay_011058_22	euxassay_011058_23	euxassay_011058_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 07 08 23 24 moderate expression: see section 09 22

lung

moderate

regional

moderate expression: see section 09 10 11 12 weak expression: see section 15 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36569
Entity Detected:	Krt71, keratin 71 ( MGI:1861586)
Sequence:	sense strand is shown >T36569 AGAACAGGAGCAGAGGTAGTGCCAGCGACTACAAGGATACCCTAACAAAGGGCTCCAGTCTGAGCACCCC CTCCAAGAAAGGTGGCCGATGAGAGGGCTTCTTGTGACCTCGATCCCTCAGCACCCTCTGTGCCCTAGCC TGTTGTCCCCCTCCCTACTCCCTTATATCATTCCTGGGCCCCTAGATGTGTCCTCAGCTAACCTCCTTCT CTCTCCAAGTGTTCTGAGCCTGGGTAGCTCTGGGCTACCATGTGGTGCACTTCTGACAGACACCAGGGGT TTCTAAGCTTCAGCTCAGGTCCAGCCTGTCCCTTCCACCAGCCTTTGCCTCCGTCCTCACCACACTACCT GGTTTCTTGGGTCTCCATCTGGGCTTCCTCGTCTGCTGAGCTTTTGTCCCAGCTCCTGTGTACAACACAA GCTAGAGCTGTACCCAAGAAGAAGACT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100619. Forward Primer - name:100619_F_cDNA_Krt2-6g, sequence:AGAACAGGAGCAGAGGTAGTGC; Reverse Primer - name:100619_N_SP6_cDNA_Krt2-6g, sequence:AGTCTTCTTCTTGGGTACAGCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11870 same embryo
	EMAGE:11872 same embryo
	EMAGE:11869 same embryo
	EMAGE:11868 same embryo
	EMAGE:11873 same embryo
	EurExpress:euxassay_011058 same experiment
	MGI:4825843 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source