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EMAGE:11885

Lrrtm2 leucine rich repeat transmembrane neuronal 2 ( MGI:2389174)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11885 EMAGE:11885 EMAGE:11885 EMAGE:11885 EMAGE:11885
"Pseudo-wholemount" of euxassay_011087. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011087_01 euxassay_011087_02 euxassay_011087_03 euxassay_011087_04
EMAGE:11885 EMAGE:11885 EMAGE:11885 EMAGE:11885 EMAGE:11885
euxassay_011087_05 euxassay_011087_06 euxassay_011087_07 euxassay_011087_08 euxassay_011087_09
EMAGE:11885 EMAGE:11885 EMAGE:11885 EMAGE:11885 EMAGE:11885
euxassay_011087_10 euxassay_011087_11 euxassay_011087_12 euxassay_011087_13 euxassay_011087_14
EMAGE:11885 EMAGE:11885 EMAGE:11885 EMAGE:11885 EMAGE:11885
euxassay_011087_15 euxassay_011087_16 euxassay_011087_17 euxassay_011087_18 euxassay_011087_19
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euxassay_011087_20 euxassay_011087_21 euxassay_011087_22 euxassay_011087_23 euxassay_011087_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11885Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11885_wholemount_strong.wlz
11885_wholemount_moderate.wlz
11885_wholemount_weak.wlz
11885_wholemount_possible.wlz
11885_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11885_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36599
Entity Detected:Lrrtm2, leucine rich repeat transmembrane neuronal 2 ( MGI:2389174)
Sequence:sense strand is shown

>T36599
GCGCTAAGGTCTCCATATGTTTTTTGGTTTTTTTTTTTACTGAAAACAGTTCCAAAAGATGCTACTAAAC
AAATATAGGTAGGGACAAAAACCTGTTTGATTATTATTCTCCATCCTATGGACCACAACTGGCTTCCTAC
TTAAGAAGGAGACAGCAAAGTTCTCTTGTAAGATAATGTGGTATTTACTCAAATTATAATTTACTGCCAG
TATAAGAAGTAGACTTACATGTGTGCTTAAGACTGGTAAATATTAAACGTTATTCTTTCAGTTTGGCTTG
GCTTAATAAACATTATCTAAGTTACATCATGAAACCAGCACCTTTTATATAACTCTAATAAAACTGGGTG
ACTCTTTAATATATTTCAACAATAAATCCAAGCAATTATAAAAAGATCTCATTATAATAAATCAATACTA
AATAACTATGCTGACTTCTTATACAACTCTAGTTTGACTTATAATGGACATGCTGATTTTTGTGGCATTT
AGCCTATTATTTTAAACTAGTATTAAATTCCATTTTACTAATTAACTTACTAAATCCTCTATTATGTAAA
AATTTGCATTTGGGAAAGAGAAAAACTGAGTCAACTTAACAATCCTGATATACTCCATCCAGTTTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70617. Forward Primer - name:070617_F_cDNA_Lrrtm2, sequence:GCGCTAAGGTCTCCATATGTTT; Reverse Primer - name:070617_N_SP6_cDNA_Lrrtm2, sequence:CAAACTGGATGGAGTATATCAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11882 same embryo
 EMAGE:11884 same embryo
 EMAGE:11887 same embryo
 EMAGE:11886 same embryo
 EMAGE:11883 same embryo
 EurExpress:euxassay_011087 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS