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EMAGE:11896

Large like-glycosyltransferase ( MGI:1342270)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896
"Pseudo-wholemount" of euxassay_011033. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011033_01 euxassay_011033_02 euxassay_011033_03 euxassay_011033_04
EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896
euxassay_011033_05 euxassay_011033_06 euxassay_011033_07 euxassay_011033_08 euxassay_011033_09
EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896
euxassay_011033_10 euxassay_011033_11 euxassay_011033_12 euxassay_011033_13 euxassay_011033_14
EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896
euxassay_011033_15 euxassay_011033_16 euxassay_011033_17 euxassay_011033_18 euxassay_011033_19
EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896 EMAGE:11896
euxassay_011033_20 euxassay_011033_21 euxassay_011033_22 euxassay_011033_23 euxassay_011033_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11896Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11896_wholemount_strong.wlz
11896_wholemount_moderate.wlz
11896_wholemount_weak.wlz
11896_wholemount_possible.wlz
11896_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11896_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
olfactory cortex marginal layer
weak weak
regionalweak expression: see section 12 13 17 18 19
facial vii ganglion
weak weak
single cellweak expression: see section 05 21
trigeminal v ganglion
weak weak
single cellweak expression: see section 04 05 06 07 09 18 19 21 22
dorsal root ganglion
weak weak
single cellweak expression: see section 09 10 11 13 14 15 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36581
Entity Detected:Large, like-glycosyltransferase ( MGI:1342270)
Sequence:sense strand is shown

>T36581
GAAATGTATCCTGCCACCTAGCTGGGTACCCATATGGACAGCAACCCAAGGACCCAAACAAACATCTGGA
GACTCTCATATGGACATTACACATGAGGGACCTGAAGGAGGGATGAATTGGTTGTTTATTCTCAAAGTCA
CTCTTGTTTTGTTTTGAGTTGTTTGAATTTGGGGGATTTTGTTCATTTTGATCTAGAAATCTAAGTTTTT
TAAAAAATCTAATCCTTAAAGCTCTGAAAGAAAAATCAACTCCCTCGATCAACACCCCTCAGCAGCAGTG
GCCCTGCAAGTCAAGCACCCGTGGAGGATGTTCCTGGGTGAACAGCTTCCTTCATGATGAACACTGAGAC
CACCTGATTCCAAGTTTCCACTCTCTAGATCCAGTACTATGACAGGGACTACAGAAATATGAGTCCAAAC
ACTATATGCAACTCACATTTTCTATGGACCATCACCAAGCCACAAGGAAAGGGCTTCATGGAACTACTTC
AGCACTCACAGAAGGTATGATTCCCTTCTTGGGCCTATAGAAGAACTTGAACAGTTCACAGAAGTCCAAT
TTGAACACTTGTATGACTACATTGAAGCCACTGGAAGAAATGGGTCAGGTATCTCTGTGTTCTTCCCTGC
CTCACTGTTGAAAGTGAGGGAGCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99214. Forward Primer - name:099214_F_cDNA_Large, sequence:GAAATGTATCCTGCCACCTAGC; Reverse Primer - name:099214_N_SP6_cDNA_Large, sequence:CAGCTCCCTCACTTTCAACAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11899 same embryo
 EMAGE:11895 same embryo
 EMAGE:11900 same embryo
 EMAGE:11897 same embryo
 EMAGE:11898 same embryo
 EurExpress:euxassay_011033 same experiment
 MGI:4825871 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS