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EMAGE:11900

Klk4 kallikrein related-peptidase 4 (prostase, enamel matrix, prostate) ( MGI:1861379)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11900 EMAGE:11900 EMAGE:11900 EMAGE:11900 EMAGE:11900
"Pseudo-wholemount" of euxassay_011026. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011026_01 euxassay_011026_02 euxassay_011026_03 euxassay_011026_04
EMAGE:11900 EMAGE:11900 EMAGE:11900 EMAGE:11900 EMAGE:11900
euxassay_011026_05 euxassay_011026_06 euxassay_011026_07 euxassay_011026_08 euxassay_011026_09
EMAGE:11900 EMAGE:11900 EMAGE:11900 EMAGE:11900 EMAGE:11900
euxassay_011026_10 euxassay_011026_11 euxassay_011026_12 euxassay_011026_13 euxassay_011026_14
EMAGE:11900 EMAGE:11900 EMAGE:11900 EMAGE:11900 EMAGE:11900
euxassay_011026_15 euxassay_011026_16 euxassay_011026_17 euxassay_011026_18 euxassay_011026_19
EMAGE:11900 EMAGE:11900 EMAGE:11900 EMAGE:11900
euxassay_011026_20 euxassay_011026_21 euxassay_011026_22 euxassay_011026_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11900Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11900_wholemount_strong.wlz
11900_wholemount_moderate.wlz
11900_wholemount_weak.wlz
11900_wholemount_possible.wlz
11900_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11900_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36560
Entity Detected:Klk4, kallikrein related-peptidase 4 (prostase, enamel matrix, prostate) ( MGI:1861379)
Sequence:sense strand is shown

>T36560
ACACTATCAGGAGCATCCCTGTGGCTACCCAATGCCCCACTCCTGGAGATACCTGCCTAGTCTCTGGTTG
GGGTCAACTAAAGAATGGGAAACTGCCCAGCCTCCTGCAGTGTGTGAATCTCTCAGTGGCGTCAGAGGAG
ACTTGCCGGCTGCTGTATGACCCTGTGTACCACCTCAGTATGTTCTGCGCTGGAGGAGGACAGGACCAAA
AGGACTCCTGCAATGGGGACTCTGGGGGTCCCATAGTGTGCAACAGATCCCTACAAGGGCTCGTGTCTAT
GGGACAAGGAAAGTGTGGGCAGCCTGGCATACCAAGTGTCTACACCAATCTCTGCAAGTTCACTAACTGG
ATCCAGACCATCATTCAGACAAACTAGCTATGGACTGACTACAAGGCCCACATGAAGCCTGCTGAGGGAA
CCTGAGACTCAGGATTGCTCCTAGCCCTTCTCCCTCAGATCTACAAGGTCAGGCCCTAGCCTCCTCCCTC
AGACTCACAAGTCCAGACTCCAATCTCCTCCCTCAGACCCACAAGTCCAGACTCCAATTTCCTCTCTCAG
ATCCAAGGATTCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100604. Forward Primer - name:100604_F_cDNA_Klk4, sequence:ACACTATCAGGAGCATCCCTGT; Reverse Primer - name:100604_N_SP6_cDNA_Klk4, sequence:CTGAATCCTTGGATCTGAGAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11899 same embryo
 EMAGE:11895 same embryo
 EMAGE:11897 same embryo
 EMAGE:11898 same embryo
 EMAGE:11896 same embryo
 EurExpress:euxassay_011026 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS